Methanobacterium formicicum, an endosymbiont of the anaerobic ciliateMetopus striatus McMurrich

Bruggen, J.J.A. van; Zwart, K.B.; Assema, Renée M. van; Stumm, Claudius K.; Vogels, Godfried G.

doi:10.1007/bf00692703

Cited by 107 publications

(50 citation statements)

References 17 publications

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“…The results strongly indicate the presence of hydrogenase activity in the microbodies of sapropelic ciliates. This study validates the hypothesis mentioned by Van Bruggen et al (1984) that these organelles might produce H7, which is utilized subsequently by the methanogenic endosymbionts, and consequently the methanogen-associated organelles may be referred to as hydrogeriosomes. Further characterization of these organelles by cytochemical techniques is being undertaken at present in order to elucidate their metabolic activities.…”

Section: Resultssupporting

confidence: 90%

Cytochemical Localization of Hydrogenase Activity in the Anaerobic Protozoa Trichomonas vaginalis, Plagiopyla nasuta and Trimyema compressum

Zwart¹,

Goosen²,

Schijnde³

et al. 1988

Microbiology

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~ ~~~~~~~~~~~~~A cytochemical staining method was developed for the demonstration of hydrogenase activity in hydrogenosomes of Trichomonas vaginalis. After fixation in low concentrations of glutaraldehyde under strictly anaerobic conditions and incubation of these cells in the presence of H2 and the tetrazolium compound 2-(2'-benzothiazolyl)-5-styryl-3-(4'-phthalhydrazidyl)-tetrazolium chloride, an electron-dense deposit was produced in the hydrogenosomes. The method was used to demonstrate the hydrogenosomal nature of similar microbodies of the sapropelic ciliates Plagiopyla nasuta and Trimyema compressum.

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Section: Resultssupporting

confidence: 90%

Cytochemical Localization of Hydrogenase Activity in the Anaerobic Protozoa Trichomonas vaginalis, Plagiopyla nasuta and Trimyema compressum

Zwart¹,

Goosen²,

Schijnde³

et al. 1988

Microbiology

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“…Id), sometimes even forming stacks indicating their intimate physiological interaction. The endosymbionts were shown to be methanogenic archaeobacteria by their characteristic fluorescence due to their content of cofactor F,,, (van Bruggen et al, 1983;Goosen et al, 1990a;Fenchel & Finlay, 1991 b;, by rRNA sequencing (Embley et al, 1992; and by cultivation (van Bruggen et al, 1984). Washed cells of several protist species harbouring the above association have been shown to produce CH,, demonstrating the functional activity of the symbionts (Wagener et al, 1990;Fenchel & Finlay, 1992).…”

Section: Free-living Ciliatesmentioning

confidence: 99%

Review Article: The hydrogenosome

Müller

1993

Journal of General Microbiology

410

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“…The anaerobic habitat and the special organelles have motivated many scientists to study the endosymbionts of Metopus , of which most focused on the methanogenic archaea (van Bruggen et al. , ; Embley and Finlay ; Embley et al. ,b; Fenchel and Finlay ; Müller ; Narayanan et al.…”

mentioning

confidence: 99%

Morphology and Phylogeny of the Soil Ciliate Metopus yantaiensis n. sp. (Ciliophora, Metopida), with Identification of the Intracellular Bacteria

Omar

Zhang

Zou

et al. 2017

J Eukaryotic Microbiology

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The morphology and infraciliature of a new ciliate, Metopus yantaiensis n. sp., discovered in coastal soil of northern China, were investigated. It is distinguished from its congeners by a combination of the following features: nuclear apparatus situated in the preoral dome; 18-21 somatic ciliary rows, of which three extend onto the preoral dome (dome kineties); three to five distinctly elongated caudal cilia, and 21-29 adoral polykinetids. The 18S rRNA genes of this new species and two congeners, Metopus contortus and Metopus hasei, were sequenced and phylogenetically analyzed. The new species is more closely related to M. hasei and the clevelandellids than to other congeners; both the genus Metopus and the order Metopida are not monophyletic. In addition, the digestion-resistant bacteria in the cytoplasm of M. yantaiensis were identified, using a 16S rRNA gene clone library, sequencing, and fluorescence in situ hybridization. The detected intracellular bacteria are affiliated with Sphingomonadales, Rhizobiales, Rickettsiales (Alphaproteobacteria), Pseudomonas (Gammaproteobacteria), Rhodocyclales (Betaproteobacteria), Clostridiales (Firmicutes), and Flavobacteriales (Bacteroidetes).

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Methanobacterium formicicum, an endosymbiont of the anaerobic ciliateMetopus striatus McMurrich

Cited by 107 publications

References 17 publications

Cytochemical Localization of Hydrogenase Activity in the Anaerobic Protozoa Trichomonas vaginalis, Plagiopyla nasuta and Trimyema compressum

Cytochemical Localization of Hydrogenase Activity in the Anaerobic Protozoa Trichomonas vaginalis, Plagiopyla nasuta and Trimyema compressum

Review Article: The hydrogenosome

Morphology and Phylogeny of the Soil Ciliate Metopus yantaiensis n. sp. (Ciliophora, Metopida), with Identification of the Intracellular Bacteria

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