Review Article: The hydrogenosome

Müller, Miklós

doi:10.1099/00221287-139-12-2879

Cited by 410 publications

(79 citation statements)

References 66 publications

(71 reference statements)

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“…0.5 g and 1 g DNA from clones 1 and 2 were used for comparison. DNA samples were denatured in 0.4N NaOH for 10 min at room temperature, neutralized in 1M NH 4 OAc and vacuum transferred onto nitrocellulose using a slot-blot apparatus. Blots were then hybridized and washed as before.…”

Section: Methodsmentioning

confidence: 99%

“…The appeal of Trichomonas from a biological viewpoint stems, in large part, from properties that reflect both its primitive nature and its parasitic lifestyle. For example, trichomonads lack two organelles typically found in eukaryotes, the mitochondrion and the peroxisome, but instead contain an organelle involved in carbohydrate metabolism called the hydrogenosome (3,4). Interestingly, the hydrogenosome and mitochondrion appear to have evolved from a common endosymbiont (5)(6)(7)(8).…”

mentioning

confidence: 99%

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Transient and selectable transformation of the parasitic protist Trichomonas vaginalis

Delgadillo

Liston

Niazi

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

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We have developed methods to transiently and selectably transform the human-infective protist Trichomonas vaginalis. This parasite, a common cause of vaginitis worldwide, is one of the earlier branching eukaryotes studied to date. We have introduced three heterologous genes into T. vaginalis by electroporation and have used the 5 and 3 untranslated regions of the endogenous gene ␣-succinyl CoA synthetase B (␣-SCSB) to drive transcription of these genes. Transient expression of two reporter proteins, chloramphenicol acetyltransferase (CAT) or luciferase, was detected when electroporating in the presence of 50 g closedcircular construct. Optimal levels of expression were observed using Ϸ2.5 ؋ 10 8 T. vaginalis cells and 350 volts, 960 Fd for electroporation; however, other conditions also led to significant reporter gene expression. A time course following the expression of CAT in T. vaginalis transient transformants revealed the highest level of expression 8-21 hr postelectroporation and showed that CAT activity is undetectable using TLC by 99 hr postelectroporation. The system we established to obtain selectable transformants uses the neomycin phosphotransferase (neo) gene as the selectable marker. Cells electroporated with 20 g of the NEO construct were plated in the presence of 50 g͞ml paromomycin and incubated in an anaerobic chamber. The paromomycin-resistant colonies that formed within 3-5 days were cultivated in the presence of drug and DNA was isolated for analyses. The NEO construct was shown to be maintained episomally, as a closed-circle, at between 10-30 copies per cell. The ability to transiently and selectably transform T. vaginalis should greatly enhance research on this important human parasite.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Transient and selectable transformation of the parasitic protist Trichomonas vaginalis

Delgadillo

Liston

Niazi

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

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“…Hydrogenase expression is clearly a prerequisite for the conversion from mitochondrion to hydrogenosome during the eukaryotic specialization to anaerobic niches. Because hydrogenosomes have arisen independently several times in mitochondrion-bearing lineages 1,4,11 , it is possible that their hydrogenases did as well.…”

mentioning

confidence: 99%

“…Hydrogenosomes are about 1 micrometre in diameter and are so called because they produce molecular hydrogen 1 . It has been postulated that hydrogenosomes evolved from mitochondria by the concomitant loss of their respiration and organellar genomes [1][2][3][4] , and so far no hydrogenosome has been found that has a genome 1,2 . Here we provide evidence for the existence of a hydrogenosomal genome of mitochondrial descent, and show that the anaerobic heterotrichous ciliate Nyctotherus ovalis possesses a new type of nuclearencoded 'iron-only' hydrogenase enzyme.…”

mentioning

confidence: 99%

A hydrogenosome with a genome

Akhmanova

Voncken

Alen

et al. 1998

Nature

254

158

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“…Recently the prebiotic synthesis of pyruvate has however been demonstrated from CO and alkyl thiols, with FeS as catalyst, at high temperature (250 C) and pressure [79]. Pyruvate can yield ATP by fermentation by substrate level phosphorylation, using the enzymes pyruvate:ferredoxin oxidoreductase, hydrogenase and succinyl-CoA synthase, with CO 2 , H 2 and acetate as end products [80]. Other types of fermentation also require only a few enzymes [81].…”

Section: Biology and The Origin Of Lifementioning

confidence: 99%

Sorption heat engines: Simple inanimate negative entropy generators

Muller

Schulze‐Makuch

2006

Physica A: Statistical Mechanics and its Applications

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The name 'sorption heat engines' is proposed for simple negative entropy generators that are driven by thermal cycling and work on alternating adsorption and desorption. These generators are in general not explicitly recognized as heat engines. Their mechanism is applicable to the fields of engineering, physics, chemistry, geology, and biology, in particular the origin of life. Four kinds of sorption heat engines are distinguished depending on the occurrence of changes in the adsorbent or adsorbate during the thermal cycle.

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Review Article: The hydrogenosome

Cited by 410 publications

References 66 publications

Transient and selectable transformation of the parasitic protist Trichomonas vaginalis

Transient and selectable transformation of the parasitic protist Trichomonas vaginalis

A hydrogenosome with a genome

Sorption heat engines: Simple inanimate negative entropy generators

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