Metallochelate immobilization of urease on to amorphous SiO2

Godjevargova, Tzonka; Velikova, M.; Vasileva, N.; Dimova, N.; Damyanov, D.

doi:10.1016/j.procbio.2005.02.008

Cited by 4 publications

(2 citation statements)

References 12 publications

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“…The XRD data in Figure 6a shows the characteristic diffraction peaks of Cu-LBMS synthesized in our work (consisting of typical layer features with a series of diffraction peaks) [32]. Figure 6b indicates that the SiO 2 spheres have a common amorphous structure [50,52]. After in situ growth of Cu-LBMS nanosheets on the silica spheres, the XRD of SiO 2 @Cu-LBMS exhibits features that are a combination of both Cu-LBMS and silica spheres.…”

Section: Resultsmentioning

confidence: 99%

A Facile Synthesis of Core-Shell SiO2@Cu-LBMS Nano-Microspheres for Drug Sustained Release Systems

2019

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A well-dispersed SiO2@Layered hydroxide cupric benzoate (SiO2@Cu-LBMS) with a hierarchical structure have been synthesized by a facile method. The layered hydroxide cupric benzoate with a structure of layered basic metal salt (Cu-LBMS) was directly deposited on the surface of silica spheres without any blinder. The morphology of the SiO2@Cu-LBMS nano-microsphere was observed by SEM, and the reaction conditions was also discussed. In addition, the XRD patterns and FTIR spectra provide consistent evidence to the formation of SiO2@Cu-LBMS nano-microspheres. The release behavior and drug loading capability of SiO2@Cu-LBMS microspheres were also investigated by using ibuprofen, aspirin and salicylic acid as model drugs. The results indicated that the drug loading capability of SiO2@Cu-LBMS nano-microspheres was much larger than layered hydroxide cupric benzoate, and the releasing time was significantly prolonged than layered hydroxide cupric benzoate and their physical mixture.

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Section: Resultsmentioning

confidence: 99%

A Facile Synthesis of Core-Shell SiO2@Cu-LBMS Nano-Microspheres for Drug Sustained Release Systems

2019

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“…In this purposes, urease has been widely immobilized to different solid support materials. For example, chitosan‐poly(glycidyl methacrylate) copolymer, poly( N ‐isopropylacrylamide‐co‐ N ‐acryloxysuccinimide‐co‐2‐hydroxyethyl methacrylate) composite hydrogel membrane, clay matrixes (Laponite and Zn‐Al layered double hydroxides), polyamide hollow‐fiber membranes, amorphous SiO 2 , poly(acrylonitrile) chitosan composite membranes, nanocomposite matrix, nanoporous TiO 2 film, modified acrylonitrile copolymer membranes, supermacroporous poly( N ‐isopropylacrylamide) cryogels, nanostructured polymer membrane, phosphonate grafted iron oxide nanoparticles, alkyl modified nano‐porous silica, functionalized gold nanoparticles, and cellulosic cotton fibers have been used for urease immobilization a support materials.…”

Section: Introductionmentioning

confidence: 99%

Reactive red 120 and NI(II) derived poly(2‐hydroxyethyl methacrylate) nanoparticles for urease adsorption

Türkcan

Uygun

Akgöl

et al. 2013

J of Applied Polymer Sci

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Non-porous poly(2-hydroxyethyl methacrylate) [p(HEMA)] nanoparticles were prepared by surfactant free emulsion polymerization. The p(HEMA) nanoparticles was about 200 nm diameter, spherical form, and non-porous. Reactive Red 120 (RR 120) was covalently attached to the p(HEMA) nanoparticles and Ni(II) ions were incorporated to attach dye molecules. Urease was immobilized onto RR120-Ni(II) attached p(HEMA) nanoparticles via adsorption. The maximum urease adsorption capacity of RR120-Ni(II) attached p(HEMA) nanoparticles was 480.01 mg g 21 nanoparticles at pH 7.0 in phosphate buffer. It was observed that urease could be repeatedly adsorbed and desorbed without significant loss in adsorption amount. K m values were 21.50 and 34.06 mM for the free and adsorbed enzyme. The V max values were 4 U for the free enzyme and 3.3 U for the adsorbed enzyme. The optimum pH was 25 mM pH 7 phosphate buffer for free and adsorbed enzyme. The optimum temperature was determined at 35 C and 55 C for the free and adsorbed enzyme, respectively. These findings show considerable promise for this material as an adsorption matrix in biotechnological applications.

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Ureases. II. Properties and their customizing by enzyme immobilizations: A review

Krajewska

2009

Journal of Molecular Catalysis B: Enzymatic

117

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Metallochelate immobilization of urease on to amorphous SiO2

Cited by 4 publications

References 12 publications

A Facile Synthesis of Core-Shell SiO2@Cu-LBMS Nano-Microspheres for Drug Sustained Release Systems

A Facile Synthesis of Core-Shell SiO2@Cu-LBMS Nano-Microspheres for Drug Sustained Release Systems

Reactive red 120 and NI(II) derived poly(2‐hydroxyethyl methacrylate) nanoparticles for urease adsorption

Ureases. II. Properties and their customizing by enzyme immobilizations: A review

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