Metal-Carbon Interactions on Reduced Graphene Oxide under Facile Thermal Treatment: Microbiological and Cell Assay

Abstract

Silver-functionalized reduced graphene oxide (Ag-rGO) nanosheets were prepared by single chemical and thermal processes, with very low concentration of silver. The resulting carbon framework consists of reduced graphene oxide (rGO) sheets or 3D networks, decorated with anchored silver nanoparticles. The Ag-rGO nanosheets were dispersed into a polymer matrix and the composites evaluated for use as biological scaffolds. The rGO material in poly(dimethylsiloxane) (PDMS) has been tested for antimicrobial activity against Gram-positiveStaphylococcus aureus(S. Aureus) bacteria, after exposure times of 24 and 120 hours, as well as in the determination of cell viability on cultures of fibroblast cells (NIH/3T3). Using 1 mL of Ag-rGO in PDMS the antibacterial effectiveness againstStaphylococcus aureuswas limited, showing an increased amount of Colony Forming Units (CFU), after 24 hours of contact. In the cell viability assay, after 48 hours of contact, the group of 1 mL of Ag-rGO with PDMS was the only group that increased cell viability when compared to the control group. In this context, it is believed these behaviors are due to the increase in cell adhesion capacity promoted by the rGO. Thus, the Ag-rGO/PDMS hybrid nanocomposite films can be used as scaffolds for tissue engineering, as they limit antimicrobial activity.

Antimicrobial and cytotoxic capacity of pyroligneous extracts films of Eucalyptus grandis and chitosan for oral applications

“…The rationale for using these film concentrations in the cytotoxicity assay comes from the knowledge that they represent the lowest and highest concentration used in the antimicrobial assay by the direct contact test. The 48-h cell exposure period was based on previous studies 52 , 53 .…”

Synthesis and applications of graphene and graphene-based nanocomposites: Conventional to artificial intelligence approaches

“…Carbon-based agents are extremely adaptable and can be employed in a variety of applications (Zeng et al, 2019;Zeng et al, 2020). In the realm of heterogeneous catalysis, graphene and graphene-based composites have received a lot of attention in recent years (Gopiraman et al, 2013a;Gopiraman et al, 2013b;Gopiraman et al, 2014;Vinoth et al, 2015;Carreño et al, 2017;Wu et al, 2020a). These composites offer a lot of flexibility when it comes to tailoring the catalytic capabilities to specific needs.…”

Comparative study of NiO/CuO/Ag doped graphene based materials for reduction of nitroaromatic compounds and degradation of dye with statistical study

“…Graphene oxide and many other composites such as those made up of Titanium and Zinc possesses intriguing applications in thermal, mechanical and in addition to electrical properties also 4 , as well as a vast surface area with a variety of functional groups, allowing for chemical changes and making it a promising research option 5 , 6 . These composites have various applications like in catalysis 5 , sensors 7 , 8 , and photocatalysis 9 , for the reduction process in organic synthesis, toxic metal ion removal 10 , 11 , cellular imaging 12 , 13 , drug delivery 12 , and many other features have been documented in the literature 14 – 18 . Graphene based materials can also be used as efficient heterogeneous catalysts 19 , 20 and its extraordinary conductivity can aid electron transport during transformations 21 .…”