Metabonomics analysis of kidneys in rats administered with chronic low‐dose cadmium by ultra‐performance liquid chromatography‐mass spectrometry

Zhang, Meiyan; Jia, Siqi; Liu, Yajing; Li, Yanli; Li, Siqi; Liu, Bo; Zhao, Xiujuan; Sun, Chongde

doi:10.1002/jat.3735

Cited by 13 publications

(6 citation statements)

References 56 publications

(69 reference statements)

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“…Two other significantly different metabolites were hypoxanthine nucleoside (M251T217) and hypoxanthine (M135T286). Hypoxanthine nucleoside was one of nucleoside compounds based on hypoxanthine, which was obtained from the reaction of adenosine with nitrous acid or enzymatic ammonolysis (Zhang et al, 2018). Hypoxanthine was produced under the action of nucleoside phosphate carboxylase.…”

Section: Metabolomics Resultsmentioning

confidence: 99%

Effects of Acute and Chronic Exposure to Semicarbazide on the Sea Cucumber Apostichopus japonicus

Tian

Zhang

et al. 2021

Front. Environ. Sci.

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The effects of acute and chronic exposure to semicarbazide were carried out on the sea cucumber Apostichopus japonicus. A half-maximal lethal concentration of 3.72 g/L of semicarbazide hydrochloride (95% confidence interval 3.43–4.02 g/L) was deduced. At 20, 4, and 2% of the half-maximal lethal concentrations, a 28-days exposure induced morphological alterations, oxidative stress, and acetylcholinesterase (AChE) activity in the respiratory tree, intestinal tract, and longitudinal muscle of A. japonicus. Exposure to 20% of the half-maximal lethal concentration resulted in lesions in the respiratory tree and disintegration in the intestinal tract. Exposure to lower concentration induced a gradual accumulation of lesions in the respiratory tree, intestinal tract, and longitudinal muscle. Levels of markers of oxidative stress and neurotransmission, including superoxide dismutase (SOD), catalase, and AChE, were increased during the initial days of exposure and then decreased. The activity of SOD, catalase, and AChE were highest in A. japonicus exposed to 4%, followed by 20 and 2% of the half-maximal lethal concentration at the same time. At the later stages of the 28-days exposure, marker levels were decreased and close to levels in the control groups. Non-targeted metabolomics indicated that significantly different metabolites were screened out, 28 in the positive ion mode and 38 in the negative ion mode, impairments in neurological function, osmotic pressure regulation, energy metabolism, and protein digestion and absorption following exposure of A. japonicus to semicarbazide. KEGG pathway enrichment showed that the exposure affected pathways related to ABC transporters, central carbon metabolism in cancer, protein digestion and absorption, aminoacyl-tRNA biosynthesis, and biosynthesis of unsaturated fatty acids.

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Section: Metabolomics Resultsmentioning

confidence: 99%

Effects of Acute and Chronic Exposure to Semicarbazide on the Sea Cucumber Apostichopus japonicus

Tian

Zhang

et al. 2021

Front. Environ. Sci.

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“…Tetranor 12-HETE as a kind of oxylipin with pro-inflammatory properties, has been documented to participate in the inflammatory response and accelerate the progression of HTN ( 27 ). Previously, it was reported that the abundance of Tetranor 12-HETE was promoted by high-dose cadmium intervention, which elicited nephrotoxicity and oxidative stress to the kidneys ( 28 ). Meanwhile, Li Q and colleagues demonstrated that plasma level of Tetranor 12-HETE was extremely elevated in patients suffering from nonalcoholic fatty liver disease ( 29 ).…”

Section: Discussionmentioning

confidence: 99%

A cross-sectional study identifying disparities in serum metabolic profiles among hypertensive patients with ISH, IDH and SDH subtypes

Shen

Wang

Yang

et al. 2023

Front. Cardiovasc. Med.

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BackgroundIt has been well acknowledged that disordered intestinal microflora and their fermented products play crucial role during the development of hypertension (HTN). Aberrant profiles of fecal bacteria have been documented in subjects with isolated systolic HTN (ISH) and isolated diastolic HTN (IDH) previously. Nevertheless, evidence regarding the association of metabolic products in the bloodstream with ISH, IDH and combined systolic and diastolic HTN (SDH) remains scarce.MethodsWe performed a cross-sectional study and conducted untargeted liquid chromatography-mass spectrometry (LC/MS) analysis on serum samples of 119 participants, including 13 subjects with normotension (SBP < 120/DBP < 80 mm Hg), 11 individuals with ISH (SBP ≥ 130/DBP < 80 mm Hg), 27 patients with IDH (SBP < 130/DBP ≥ 80 mm Hg), and 68 SDH patients (SBP ≥ 130, DBP ≥ 80 mm Hg).ResultsHere, the results showed clearly separated clusters in PLS-DA and OPLS-DA score plots for patients suffering from ISH, IDH and SDH when compared with normotension controls. The ISH group was characterized by elevated levels of 3,5-tetradecadien carnitine and notable reduction of maleic acid. While IDH patients were enriched with metabolites in L-lactic acid and depleted in citric acid. Stearoylcarnitine was identified to be specifically enriched in SDH group. The differentially abundant metabolites between ISH and controls were involved in tyrosine metabolism pathways, and in biosynthesis of phenylalanine for those between SDH and controls. Potential linkages between the gut microbial and serum metabolic signatures were detected within ISH, IDH and SDH groups. Furthermore, we found the association of discriminatory metabolites with the characteristics of patients.ConclusionOur findings demonstrate disparate blood metabolomics signatures across ISH, IDH and SDH, with differentially enriched metabolites and potential functional pathways identified, reveal the underlying microbiome and metabolome network in HTN subtypes, and provide potential targets for disease classification and therapeutic strategy in clinical practice.

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“…Metabolomics analyzes the changes of the total metabolites of biological samples affected by the environment. This helps to understand how animals and plants respond to various hostile factors at the molecular level [ 6 ]. At present, it is widely used in the study of environmental pollutants, such as investigating the mechanism of Cd poisoning in rat urine [ 7 ] and kidneys [ 6 ], which provides the possibility for directly evaluating the health effects of low-dose environmental poisons [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Cadmium on Liver Function and its Metabolomics Profile in the Guizhou Black Goat

Shen

2023

Metabolites

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Cadmium (Cd) is a toxic heavy metal, which will lead to ecosystem contamination, threatening the life of grazing animals. Goats are an important grazing animal biomarker to evaluate Cd toxicity, but the effect of short-term and high-concentration Cd toxicity on goat liver function and its latent mechanism is still unclear. A total of ten male Guizhou black goats were randomly divided into two groups: CON group, sterilized tap water (no CdCl2), and Cd group (20 mg Cd·kg−1·BW, CdCl2⋅2.5H2O). The test lasted for 30 days. In this study, we found that Cd poisoning in drinking water affected significantly the distribution of Cd in the goat offal and tissues, and damaged the goat’s immune function of the liver. With a metabolomics approach, 59 metabolites were identified. Metabolomics analysis suggested that Cd affected lipid and amino acid metabolism of the goat liver. Collectively, our results confirmed the effect of Cd on liver function and liver metabolism, and provided insights on the molecular basis for early warnings of Cd poisoning in goats.

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Metabonomics analysis of kidneys in rats administered with chronic low‐dose cadmium by ultra‐performance liquid chromatography‐mass spectrometry

Cited by 13 publications

References 56 publications

Effects of Acute and Chronic Exposure to Semicarbazide on the Sea Cucumber Apostichopus japonicus

Effects of Acute and Chronic Exposure to Semicarbazide on the Sea Cucumber Apostichopus japonicus

A cross-sectional study identifying disparities in serum metabolic profiles among hypertensive patients with ISH, IDH and SDH subtypes

Effects of Cadmium on Liver Function and its Metabolomics Profile in the Guizhou Black Goat

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