Metabolomics of Early Stage Plant Cell–Microbe Interaction Using Stable Isotope Labeling

Pang, Qiuying; Zhang, Tong; Wang, Yan; Kong, Wenwen; Guan, Qijie; Yan, Xiufeng; Chen, Sixue

doi:10.3389/fpls.2018.00760

Cited by 32 publications

(25 citation statements)

References 64 publications

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“…On the contrary, the approach we adopted (i.e., incorporation of isotopic labeling with untargeted metabolomics) predicted the labeling pattern of each individual metabolite species detected, giving a more comprehensive ability to track each metabolite's fate (Huang et al, 2014). Similar approaches have been used to track metabolites produced by Pseudomonas syringae during interaction with Arabidopsis epidermal cells on the leaves (Pang et al, 2018). Our result suggested that a significant number of detected molecular features (202 out of 518 in total) in Pisolithus-treated roots are highly 13 C-enriched, implying that these 13 C-labeled metabolites are putatively originated from the ECM fungus even after only 24 h of interaction, despite the low detection intensity.…”

Section: Discussionmentioning

confidence: 99%

The Influence of Contrasting Microbial Lifestyles on the Pre-symbiotic Metabolite Responses of Eucalyptus grandis Roots

et al. 2019

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Plant roots co-inhabit the soil with a diverse consortium of microbes of which a number attempt to enter symbiosis with the plant. These microbes may be pathogenic, mutualistic, or commensal. Hence, the health and survival of plants is heavily reliant on their ability to perceive different microbial lifestyles and respond appropriately. Emerging research suggests that there is a pivotal role for plant root secondary metabolites in responding to microbial colonization. However, it is largely unknown if plants are able to differentiate between microbes of different lifestyles and respond differently during the earliest stages of pre-symbiosis (i.e., prior to physical contact). In studying plant responses to a range of microbial isolates, we questioned: (1) if individual microbes of different lifestyles and species caused alterations to the plant root metabolome during pre-symbiosis, and (2) if these early metabolite responses correlate with the outcome of the symbiotic interaction in later phases of colonization. We compared the changes of the root tip metabolite profile of the model tree Eucalyptus grandis during pre-symbiosis with two isolates of a pathogenic fungus (Armillaria luteobubalina), one isolate of a pathogenic oomycete (Phytophthora cinnamomi), two isolates of an incompatible mutualistic fungus (Suillus granulatus), and six isolates of a compatible mutualistic fungus (Pisolithus microcarpus). Untargeted metabolite profiling revealed predominantly positive root metabolite responses at the pre-symbiosis stage, prior to any observable phenotypical changes of the root tips. Metabolite responses in the host tissue that were specific to each microbial species were identified. A deeper analysis of the root metabolomic profiles during pre-symbiotic contact with six strains of P. microcarpus showed a connection between these early metabolite responses in the root with later colonization success. Further investigation using isotopic tracing revealed a portion of metabolites found in root tips originated from the fungus. RNA-sequencing also showed that the plant roots undergo complementary transcriptomic reprogramming in response to the fungal stimuli. Taken together, our results demonstrate that the early metabolite responses of plant roots are partially selective toward the lifestyle of the interacting microbe, and that these responses can be crucial in determining the outcome of the interaction.

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Section: Discussionmentioning

confidence: 99%

The Influence of Contrasting Microbial Lifestyles on the Pre-symbiotic Metabolite Responses of Eucalyptus grandis Roots

et al. 2019

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“…Many integrated techniques of NMR have been applied to identify the structural units of unknown metabolites. Recently developed advanced tools for plant metabolomics are one-and two-dimensional NMR [28], isotope-labeled NMR [29], and micro-coil NMR [30]. NMR is the only tool that can detect the specific labelling of stable isotopes [3].…”

Section: Advanced Tools For Analytical Research In Plant Metabolomicsmentioning

confidence: 99%

Metabolomics: A Way Forward for Crop Improvement

et al. 2019

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Metabolomics is an emerging branch of “omics” and it involves identification and quantification of metabolites and chemical footprints of cellular regulatory processes in different biological species. The metabolome is the total metabolite pool in an organism, which can be measured to characterize genetic or environmental variations. Metabolomics plays a significant role in exploring environment–gene interactions, mutant characterization, phenotyping, identification of biomarkers, and drug discovery. Metabolomics is a promising approach to decipher various metabolic networks that are linked with biotic and abiotic stress tolerance in plants. In this context, metabolomics-assisted breeding enables efficient screening for yield and stress tolerance of crops at the metabolic level. Advanced metabolomics analytical tools, like non-destructive nuclear magnetic resonance spectroscopy (NMR), liquid chromatography mass-spectroscopy (LC-MS), gas chromatography-mass spectrometry (GC-MS), high performance liquid chromatography (HPLC), and direct flow injection (DFI) mass spectrometry, have sped up metabolic profiling. Presently, integrating metabolomics with post-genomics tools has enabled efficient dissection of genetic and phenotypic association in crop plants. This review provides insight into the state-of-the-art plant metabolomics tools for crop improvement. Here, we describe the workflow of plant metabolomics research focusing on the elucidation of biotic and abiotic stress tolerance mechanisms in plants. Furthermore, the potential of metabolomics-assisted breeding for crop improvement and its future applications in speed breeding are also discussed. Mention has also been made of possible bottlenecks and future prospects of plant metabolomics.

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“…The interaction between plants and microorganisms is known to triggers signaling cascade and metabolic responses. However, the main issue of analyzing changes in the plant metabolic profile during the interaction plant cell-microorganism is avoiding contamination with metabolites from the coexisting microorganism (Pang et al 2018). A very recent work addressed this issue by implementing an effective stable isotope labeling approach coupled to LC-MRM-MS measurements (Pang et al 2018).…”

Section: The Guard Cell Metabolome During Plant Cellmicroorganism Intmentioning

confidence: 99%

Metabolomics for understanding stomatal movements

Medeiros

Luz

Oliveira

et al. 2019

Theor. Exp. Plant Physiol.

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Stomata control the exchange of CO 2 and water in land plants. For this reason, plants evolved to quickly respond the surrounding environment and endogenous cues in order to maintain their photosynthetic rates, but avoiding excessive water loss. Although guard cell has been used as model for characterization of signaling pathways, mainly regarding abscisic acid (ABA) response, several important questions about its functioning remain elusive. Recently, transcriptomics, proteomics, and metabolomics studies carried out using guard cells as model have contributed substantially for our understanding on how guard cells sense and respond to relative air humidity, CO 2 , ABA, and sucrose. Comparatively, proteomics and metabolomics platforms need substantial improvement to increase the number of analytes detected. However, with the introduction of metabolomics-based technologies, several studies have been published increasing our knowledge on guard cell function. Here, we review these new exciting findings as well as discuss the importance of using these new data to improve prediction when modelling stomatal behavior.

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Metabolomics of Early Stage Plant Cell–Microbe Interaction Using Stable Isotope Labeling

Cited by 32 publications

References 64 publications

The Influence of Contrasting Microbial Lifestyles on the Pre-symbiotic Metabolite Responses of Eucalyptus grandis Roots

The Influence of Contrasting Microbial Lifestyles on the Pre-symbiotic Metabolite Responses of Eucalyptus grandis Roots

Metabolomics: A Way Forward for Crop Improvement

Metabolomics for understanding stomatal movements

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