Metabolomics biotechnology, applications, and future trends: a systematic review

Yang, Qiang; Zhang, Aihua; Jin, Miao; Sun, Hui; Han, Ying; Ge, Yan; Wu, Fangfang; Wang, Xijun

doi:10.1039/c9ra06697g

Cited by 118 publications

(58 citation statements)

References 136 publications

(145 reference statements)

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“…Metabolomics studies directly reflect the metabolic pathways involved in the production of metabolites and yield essential information regarding the underlying biological system [ 1 ]. They mainly involve processing of biofluids such as blood, urine, and fecal samples, followed by analysis of metabolites from various metabolic pathway matrices and products [ 2 ]. There are many examples of metabolomics research on human biofluids and tissues, as well as, on animals including experimental animals such as mice and livestock [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Exploration of metabolite profiles in the biofluids of dairy cows by proton nuclear magnetic resonance analysis

Kim

Eom

et al. 2021

PLoS ONE

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Studies that screen for metabolites produced in ruminants are actively underway. We aimed to evaluate the metabolic profiles of five biofluids (ruminal fluid, serum, milk, urine, and feces) in dairy cow by using proton nuclear magnetic resonance (1H-NMR) and provide a list of metabolites in each biofluid for the benefit of future research. We analyzed the metabolites in five biofluids from lactating cows using proton nuclear magnetic resonance imaging; 96, 73, 88, 118, and 128 metabolites were identified in the five biofluids, respectively. In addition, 8, 6, 9, and 17 metabolites were unique to ruminal fluid, serum, milk, and urine, respectively. The metabolites present at high concentrations were: acetate, propionate, and butyrate in ruminal fluid; lactate, glucose, and acetate in serum; and lactose, guanidoacetate, and glucitol in milk. In addition, the following metabolites were present at high concentrations: hippurate, urea, and trimethylamine N-oxide in urine and acetate, propionate, and butyrate in feces. The score plots of the principal component analysis did not show clear distinctions among the five biofluid samples. The purpose of this study was to verify the ability of our metabolomics approaches to identify metabolites in the biofluids of dairy cows.

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Section: Introductionmentioning

confidence: 99%

Exploration of metabolite profiles in the biofluids of dairy cows by proton nuclear magnetic resonance analysis

Kim

Eom

et al. 2021

PLoS ONE

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“…[79][80][81][82][83][84][85][86] The metabolic biomarkers and pathways are crucial to revealing the pathological mechanism [87][88][89][90][91][92][93][94][95] and insight into the pharmacological targets of natural products. [96][97][98][99][100][101][102][103][104][105][106][107][108] The TCA cycle is the ultimate universal oxidation pathway for fats, carbohydrates, and amino acids, and is the key most central pathway linking almost all individual metabolic pathways. In addition, the TCA cycle is closely related to some liver diseases.…”

Section: Discussionmentioning

confidence: 99%

Functional metabolomics using UPLC-Q/TOF-MS combined with ingenuity pathway analysis as a promising strategy for evaluating the efficacy and discovering amino acid metabolism as a potential therapeutic mechanism-related target for geniposide against alcoholic liver disease

et al. 2020

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“…Some of the limitations and tasks that faced metabolomics 10 years ago [ 78 ] have now been solved, but there are also many tasks to be solved [ 48 , 58 , 79 , 80 ]. Mass spectrometers, nuclear magnetic resonance (NMR) spectrometers, and other equipment that are required to conduct the metabolomic study are expensive and require experienced staff to operate this equipment.…”

Section: Analytical Limitationsmentioning

confidence: 99%

Metabolomic Laboratory-Developed Tests: Current Status and Perspectives

et al. 2021

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Laboratory-developed tests (LDTs) are a subset of in vitro diagnostic devices, which the US Food and Drug Administration defines as “tests that are manufactured by and used within a single laboratory”. The review describes the emergence and history of LDTs. The current state and development prospects of LDTs based on metabolomics are analyzed. By comparing LDTs with the scientific metabolomics study of human bio samples, the characteristic features of metabolomic LDT are shown, revealing its essence, strengths, and limitations. The possibilities for further developments and scaling of metabolomic LDTs and their potential significance for healthcare are discussed. The legal aspects of LDT regulation in the United States, European Union, and Singapore, demonstrating different approaches to this issue, are also provided. Based on the data presented in the review, recommendations were made on the feasibility and ways of further introducing metabolomic LDTs into practice.

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Metabolomics biotechnology, applications, and future trends: a systematic review

Abstract: Given the highly increased incidence of human diseases, a better understanding of the related mechanisms regarding endogenous metabolism is urgently needed.

Cited by 118 publications

References 136 publications

Exploration of metabolite profiles in the biofluids of dairy cows by proton nuclear magnetic resonance analysis

Exploration of metabolite profiles in the biofluids of dairy cows by proton nuclear magnetic resonance analysis

Functional metabolomics using UPLC-Q/TOF-MS combined with ingenuity pathway analysis as a promising strategy for evaluating the efficacy and discovering amino acid metabolism as a potential therapeutic mechanism-related target for geniposide against alcoholic liver disease

Metabolomic Laboratory-Developed Tests: Current Status and Perspectives

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