Metabolomic Laboratory-Developed Tests: Current Status and Perspectives

Lichtenberg, Steven; Trifonova, Oxana P.; Maslov, Dmitry L.; Balashova, Elena E.; Lokhov, Petr G.

doi:10.3390/metabo11070423

Cited by 19 publications

(15 citation statements)

References 94 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In total, almost 70% of the global biochemical phenotype of the organism is estimated. Therefore, a metabogram can potentially be considered a personalized metabolomics tool [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

Mass Spectrometric Blood Metabogram: Acquisition, Characterization, and Prospects for Application

Lokhov

Balashova

Trifonova

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

In metabolomics, many metabolites are measured simultaneously in a single run. Such analytical performance opens up prospects for clinical laboratory diagnostics. In this work, a mass spectrometric metabogram was developed as a simplified and clinically applicable way of measuring the blood plasma metabolome. To develop the metabogram, blood plasma samples from healthy male volunteers (n = 48) of approximately the same age, direct infusion mass spectrometry (DIMS) of the low molecular fraction of samples, and principal component analysis (PCA) of the mass spectra were used. The seven components of the metabogram defined by PCA, which cover ~70% of blood plasma metabolome variability, were characterized using a metabolite set enrichment analysis (MSEA) and clinical test results of participating volunteers. It has been established that the components of the metabogram are functionally related groups of the blood metabolome associated with regulation, lipid–carbohydrate, and lipid–amine blood components, eicosanoids, lipid intake into the organism, and liver function thereby providing a lot of clinically relevant information. Therefore, metabogram provides the possibility to apply the metabolomics performance in the clinic. The features of the metabogram are also discussed in comparison with the thin-layer chromatography and with the analysis of blood metabolome by liquid chromatography combined with mass spectrometry.

show abstract

“…In total, almost 70% of the global biochemical phenotype of the organism is estimated. Therefore, a metabogram can potentially be considered a personalized metabolomics tool [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

Mass Spectrometric Blood Metabogram: Acquisition, Characterization, and Prospects for Application

Lokhov

Balashova

Trifonova

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…The datasets generated from clinical trials and research studies curate for clinical databases that support diagnosis and therapeutic assessment of several disease states [7]. However, these datasets require standardization and validation to create a clinical reference guide that is applicable for any biofluid, as well as FDA approval for utilizing this platform for clinical diagnostic purposes [11].…”

Section: Metabolomics In Clinical Urinalysismentioning

confidence: 99%

Application of Urine Metabolomics as a Marker in Health and Disease

Pellissery¹,

Vinayamohan²,

Viju³

et al. 2024

Advances and Challenges in Urine Laboratory Analysis

View full text Add to dashboard Cite

Advances in metabolomics research have yielded an avenue for utilizing this laboratory-based modality as a platform for clinical diagnosis, identification of novel biomarkers, and longitudinally monitoring the health status of individuals from normal physiological and pathophysiological perspectives. This chapter provides insight on the application of urinalysis in health and disease from the standpoint of deciphering a larger span of metabolite and biomarker identification using metabolomics, specifically focusing on infectious diseases, oncology, metabolic, and inflammatory diseases in humans.

show abstract

“…The personalized metabolomics analysis should be scalable, fast, and understandable to wider range of people. While a metabolomics study is unique, it can take from several months to several years, and with very complex results that are intended for scientists with experience in the same field of science [ 12 , 20 ]. Figure 3 below shows the traditional needs for the analytical methods to address both the metabolomics study and personalized metabolomics, with a focus on the differences, including the standardization and treatment of data.…”

Section: The Bottle Necks Of Personalized Metabolomicsmentioning

confidence: 99%

Current State and Future Perspectives on Personalized Metabolomics

et al. 2023

Self Cite

View full text Add to dashboard Cite

Metabolomics is one of the most promising ‘omics’ sciences for the implementation in medicine by developing new diagnostic tests and optimizing drug therapy. Since in metabolomics, the end products of the biochemical processes in an organism are studied, which are under the influence of both genetic and environmental factors, the metabolomics analysis can detect any changes associated with both lifestyle and pathological processes. Almost every case-controlled metabolomics study shows a high diagnostic accuracy. Taking into account that metabolomics processes are already described for most nosologies, there are prerequisites that a high-speed and comprehensive metabolite analysis will replace, in near future, the narrow range of chemical analyses used today, by the medical community. However, despite the promising perspectives of personalized metabolomics, there are currently no FDA-approved metabolomics tests. The well-known problem of complexity of personalized metabolomics data analysis and their interpretation for the end-users, in addition to a traditional need for analytical methods to address the quality control, standardization, and data treatment are reported in the review. Possible ways to solve the problems and change the situation with the introduction of metabolomics tests into clinical practice, are also discussed.

show abstract

Metabolomic Laboratory-Developed Tests: Current Status and Perspectives

Cited by 19 publications

References 94 publications

Mass Spectrometric Blood Metabogram: Acquisition, Characterization, and Prospects for Application

Mass Spectrometric Blood Metabogram: Acquisition, Characterization, and Prospects for Application

Application of Urine Metabolomics as a Marker in Health and Disease

Current State and Future Perspectives on Personalized Metabolomics

Contact Info

Product

Resources

About