Metabolomics: Approaches to assessing oocyte and embryo quality

Singh, R. N.; Sinclair, Kevin D.

doi:10.1016/j.theriogenology.2007.04.007

Cited by 122 publications

(68 citation statements)

References 27 publications

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“…This approach has also been applied to the study of human embryos [8,40,42] and oocytes [44]. More recently, the development of a screening technology using Raman and near-infrared spectroscopy was used to detect biomarkers in spent culture medium, with differences in algorithms generated for positive compared to negative in vitro fertilization outcomes [38,41].…”

Section: Discussionmentioning

confidence: 99%

Identification of biochemical differences between different forms of male infertility by nuclear magnetic resonance (NMR) spectroscopy

Jayaraman

Ghosh

Sengupta

et al. 2014

J Assist Reprod Genet

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Purpose The aim of this study was to analyze the seminal plasma of patients with idiopathic/male factor infertility and healthy controls with proven fertility by NMR spectroscopy, with a hope of establishing difference in biomarker profiles, if any, between the groups. Methods A total of 103 subjects visiting the infertility clinic of Manipal University with normozoospermic parameters, oligozoospermia, asthenozoospermia, azoospermia and teratozoospermia were included. Semen characteristics were analysed by standard criteria. Seminal plasma was subjected to NMR spectroscopy at a 700 MHz

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Section: Discussionmentioning

confidence: 99%

Identification of biochemical differences between different forms of male infertility by nuclear magnetic resonance (NMR) spectroscopy

Jayaraman

Ghosh

Sengupta

et al. 2014

J Assist Reprod Genet

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“…Glycolytic intermediates and amino acids are often used as markers when follicular fluid or culture media metabolites are non-invasively tested to quantify oocyte and embryo metabolism. It was found that the assessment of these markers may be used as a useful predictor of pregnancy outcome following embryo transfer in mammals (Singh and Sinclair, 2007;Revelli et al, 2009;Royere et al, 2009). In conclusion, morphometry analyses and the assessment of metabolomic markers are at present the most frequently used methods to detrmine embryo viability.…”

Section: Non-invasive Methods For Quality Assessment Of Oocytes/embryosmentioning

confidence: 99%

Selected molecular and microfluidic aspects of mammalian oocyte maturation-perspectives: a review

Kempisty¹,

Bukowska²,

Piotrowska³

et al. 2011

Vet. Med. - Czech

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ABSTRACT:Maturation of oocytes is the most important stage, which determines the subsequent successful fertilization, zygote formation, attainment of blastocyst stage, normal embryo growth and development, as well as appropriate implantation. Since in some species of mammals the efficiency of IVM maturation is still very low, many studies have focused on new combinations of media supplements. In some species of mammals, including pigs, mice, bovines, goats and dogs, EGF, BSA, and progesterone are successfully used as an enhancer of the IVC system. Application of the Lab-on-Chip system in reproductive biology opens new possibilities for the development of techniques available for the assessment of the developmental competency or potency manifested by mammalian oocytes and embryos. In most cases, the Lab-on-Chip system was used in mice. However, an increasing number of examples are available in which the system has been applied to evaluate porcine and bovine gametes and embryos. In this article, the role of EGF, BSA and progesterone is described in relation to maturation efficiency of mammalian oocytes. Moreover, the possibilities of using microfluidics (Lab-on-Chip) for the detection of oocyte developmental competency are also shown.

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“…Furthermore, morphologically normal embryos can be genetically abnormal, as a significant proportion of aneuploid embryos is capable of achieving the highest morphological scores (Singh & Sinclair 2007, Alfarawati et al 2011, Assou et al 2011.…”

Section: Introductionmentioning

confidence: 99%

Prediction of embryo implantation potential by mass spectrometry fingerprinting of the culture medium

Cortezzi¹,

Cabral²,

Trevisan³

et al. 2013

Reproduction

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This study has evaluated the performance of a multivariate statistical model to predict embryo implantation potential by processing data from the chemical fingerprinting of culture medium samples used for human embryo culture. The culture medium for 113 embryos from 55 patients undergoing ICSI was collected after embryo transfer. The samples were split into positive (nZ29) and negative (nZ84) implantation groups according their implantation outcomes (100% or 0% implantation). The samples were individually diluted and analyzed by electrospray ionization mass spectrometry (ESI-MS). The m/z ratios and relative abundances of the major ions in each spectrum were considered for partial least square discriminant analysis. Data were divided into two subsets (calibration and validation), and the models were evaluated and applied to the validation set. A total of 5987 ions were observed in the groups. The multivariate statistical model described more than 82% of the data variability. Samples of the positive group were correctly identified with 100% probability and negative samples with 70%. The culture media used for embryos that were positive or negative for successful implantation showed specific biochemical signatures that could be detected in a fast, simple, and noninvasive way by ESI-MS. To our knowledge, this is the first report that uses MS fingerprinting to predict human embryo implantation potential. This biochemical profile could help the selection of the most viable embryo, improving single-embryo transfer and thus eliminating the risk and undesirable outcomes of multiple pregnancies.

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Metabolomics: Approaches to assessing oocyte and embryo quality

Cited by 122 publications

References 27 publications

Identification of biochemical differences between different forms of male infertility by nuclear magnetic resonance (NMR) spectroscopy

Identification of biochemical differences between different forms of male infertility by nuclear magnetic resonance (NMR) spectroscopy

Selected molecular and microfluidic aspects of mammalian oocyte maturation-perspectives: a review

Prediction of embryo implantation potential by mass spectrometry fingerprinting of the culture medium

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