Metabolomic shifts inBrassica napuslines with enhancedBnPLC2expression impact their response to low temperature stress and plant pathogens

Abstract

Phosphatidylinositol-specific phospholipase C2 (PLC2) is a signaling enzyme with hydrolytic activity against membrane-bound phosphoinositides. It catalyzes the cleavage of phosphatidylinositol(4,5)bisphosphate (PtdIns(4,5) P 2 ) into two initial second messengers, myo-inositol-1,4,5-trisphosphate (InsP 3 ) and diacylglycerol (DAG). The former, as well as its fully phosphorylated derivative, myo-inositol-1,2,3,4,5,6-hexakisphosphate (InsP 6 ), play a major role in calcium signaling events within the cell, while DAG may be used in the regeneration of phospholipids or as a precursor for phosphatidic acid (PA) biosynthesis, an important signaling molecule involved in both biotic and abiotic types of stress tolerance. Overexpression of the gene for Brassica napus phospholipase C2 (BnPLC2) in Brassica napus has been shown to enhance drought tolerance, modulate multiple genes involved in different processes and favorably affect hormonal levels in different tissues. We, therefore, undertook the current study with a view to examining, at the metabolome level, its effect on both abiotic (low temperature) and biotic (stem white rot disease) types of stress in canola. Thus, while transgenic plants exhibited a significant rise in maltose levels and a concomitant elevation in some unsaturated free fatty acids (FFAs), glycerol, and glycerol 3-phosphate under subzero temperatures, they accumulated high levels of raffinose, stachyose and other sugars as well as some flavonoids under acclimatization conditions. Collectively, overexpression of BnPLC2 appears to have triggered different metabolite patterns consistent with its abiotic and, to a limited extent, biotic stress tolerance phenotypes.

Omics: The way forward to enhance abiotic stress tolerance inBrassica napusL

“…Therefore, a precise, measurable mapping of metabotypes is permitted to detect candidate gene. 176,179 Metabolic levels of fatty acids, G3P, glycerol, and maltose significantly rose in GE plants in cold stresses by elevated concentrations of flavonoids, raffinose, and other sugars. 181 Nuclear magnetic resonance (NMR)-based seed metabolites identification was carried out between two species, rapeseed and turnip.…”

Physiological and Molecular Alterations Promoted by Schizotetranychus oryzae Mite Infestation in Rice Leaves

“…This enzyme cleaves phosphatidylinositol (4,5) bisphosphate into two initial secondary messengers, myo-inositol-1,4,5-trisphosphate and diacylglycerol (DAG). DAG may be used as a precursor for phosphatidic acid (PA) biosynthesis, an important signaling molecule involved in biotic stress tolerance 94 . Protein 3,4-dihydroxy-2-butanone kinase is involved in flavin biosynthesis.…”

An Insight into the Responses of Early-Maturing Brassica napus to Different Low-Temperature Stresses

“…Xin He 1,2,3 1 Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China, Hunan Agricultural University, Changsha, Hunan, China 2 Oil Crops Research, Hunan Agricultural University, Changsha, Hunan, China 3 Hunan Branch of National Oilseed Crops Improvement Center, Changsha, Hunan, China *Address all correspondence to: hexinzhsh@126.com late maturing and early maturing) based on transcriptomics, metabolomics, lipidomics, and QTL analyses [11,25,41,42,45,[67][68][69]. Tough there were so many candidate genes involved in the response to low-temperature stress have been identified, only few genes' functions in cold tolerant have been tested and verified in rapeseed [10,29,55,70,71]. It is a pity that constitutive overexpression of rapeseed BnCBF5 and BnCBF17 resulted in various degrees of dwarf habit and longer time to flower, tough which resulted in increased freezing tolerance remarkably in spring rapeseed "Westar" [29].…”