Metabolomic change and pathway profiling reveal enhanced ansamitocin P-3 production in Actinosynnema pretiosum with low organic nitrogen availability in culture medium

Liu, Ting; Yang, Linbing; Chen, Jun; Hu, Fengxian; Wei, Liujing; Hua, Qiang

doi:10.1007/s00253-020-10463-9

Cited by 4 publications

(4 citation statements)

References 57 publications

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“…A. pretiosum is also reported to be associated with the root of Putterlickia verrucosa, a shrub that is distributed in the coastal areas of South Africa, Eswatini and Mozambique [36]. Most of the studies regarding A. pretiosum have been focused on its culture strategies, metabolic pathways, and ansamitocin producing ability [37][38][39][40][41][42]; however, there is still no information regarding the role of A. pretiosum in plant growth and response to environmental stresses. Since A. pretiosum was abundantly found in the root of halophytic C. gigantea and S. littoreus, it would be interesting to evaluate whether its association with halophyte roots contributes to plant tolerance against salinity and nutrient deficiencies.…”

Section: Discussionmentioning

confidence: 99%

Soil Mineral Composition and Salinity Are the Main Factors Regulating the Bacterial Community Associated with the Roots of Coastal Sand Dune Halophytes

Geraldi

Khoa

et al. 2022

Biology

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Soil salinity and mineral deficiency are major problems in agriculture. Many studies have reported that plant-associated microbiota, particularly rhizosphere and root microbiota, play a crucial role in tolerance against salinity and mineral deficiency. Nevertheless, there are still many unknown parts of plant–microbe interaction, especially regarding their role in halophyte adaptation to coastal ecosystems. Here, we report the bacterial community associated with the roots of coastal sand dune halophytes Spinifex littoreus and Calotropis gigantea, and the soil properties that affect their composition. Strong correlations were observed between root bacterial diversity and soil mineral composition, especially with soil Calcium (Ca), Titanium (Ti), Cuprum (Cu), and Zinc (Zn) content. Soil Ti and Zn content showed a positive correlation with bacterial diversity, while soil Ca and Cu had a negative effect on bacterial diversity. A strong correlation was also found between the abundance of several bacterial species with soil salinity and mineral content, suggesting that some bacteria are responsive to changes in soil salinity and mineral content. Some of the identified bacteria, such as Bacillus idriensis and Kibdelosporangium aridum, are known to have growth-promoting effects on plants. Together, the findings of this work provided valuable information regarding bacterial communities associated with the roots of sand dune halophytes and their interactions with soil properties. Furthermore, we also identified several bacterial species that might be involved in tolerance against stresses. Further work will be focused on isolation and transplantation of these potential microbes, to validate their role in plant tolerance against stresses, not only in their native hosts but also in crops.

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Section: Discussionmentioning

confidence: 99%

Soil Mineral Composition and Salinity Are the Main Factors Regulating the Bacterial Community Associated with the Roots of Coastal Sand Dune Halophytes

Geraldi

Khoa

et al. 2022

Biology

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“…The ASA pathway has been extensively studied because AHBA is the key precursor for synthesizing a great diversity of secondary metabolites such as ansamycins and mitomycins. The relevance of the availability of AHBA as a key structural unit for the synthesis of ansamycins and mitomycins has been demonstrated by applying different omics such as genomics, transcriptomics, metabolomics, and fluxomics (Kogure et al., 2016 ; Liu et al., 2020b ; Wang et al., 2017 ; Zhang et al., 2017 ). Several metabolic engineering strategies have been developed to increase the availability of AHBA in several species of Streptomyces and A. mediterranei , resulting in the efficient optimization in the production of target compounds such as ansamycins, geldanamycin, and rifamycins.…”

Section: Discussionmentioning

confidence: 99%

“…Remarkably, the successful heterologous expression of AHBA biosynthetic genes in E. coli has provided the basis for further genetic and metabolic manipulations of AHBA-derived polyketides. Additionally, the construction of genome-scale models resulting from omic studies, its validation by predicting microbial growth under controlled conditions, and evaluating the role of critical genes in the synthesis of compounds such as ansamitocins (T. Liu et al., 2020b ) could provide a valuable basis for the rational modification of the biosynthetic metabolism of AHBA and further elongation and modification steps in the biosynthesis of ansamycins and mitomycins antibiotics.…”

Section: Discussionmentioning

confidence: 99%

The aminoshikimic acid pathway in bacteria as source of precursors for the synthesis of antibacterial and antiviral compounds

Escalante

Mendoza-Flores

Gosset

et al. 2021

Journal of Industrial Microbiology and Biotechnology

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The aminoshikimic (ASA) pathway comprises a series of reactions resulting in the synthesis of 3-amino-5-hydroxybenzoic acid (AHBA), present in bacteria such as Amycolaptosis mediterranei and Streptomyces. AHBA is the precursor for synthesizing the mC7N units, the characteristic structural component of ansamycins and mitomycins antibiotics, compounds with important antimicrobial and anticancer activities. Furthermore, aminoshikimic acid, another relevant intermediate of the ASA pathway, is an attractive candidate for a precursor for oseltamivir phosphate synthesis, the most potent anti-influenza neuraminidase inhibitor treatment of both seasonal and pandemic influenza. This review discusses the relevance of the key intermediate AHBA as a scaffold molecule to synthesize diverse ansamycins and mitomycins. We describe the structure and control of the expression of the model biosynthetic cluster rif in A. mediterranei to synthesize ansamycins and review several current pharmaceutical applications of these molecules. Additionally, we discuss some relevant strategies developed for overproducing these chemicals, focusing on the relevance of the ASA pathway intermediates kanosmaine, AHAB and ASA.

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“…26 However, due to the high price of AP-3, most of the current reports on AP-3 have focused on increasing its production. [27][28][29][30] Liposomal formulation of AP-3 has not been reported yet.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic fabrication of photo-responsive Ansamitocin P-3 loaded liposomes for the treatment of breast cancer

et al. 2023

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Metabolomic change and pathway profiling reveal enhanced ansamitocin P-3 production in Actinosynnema pretiosum with low organic nitrogen availability in culture medium

Cited by 4 publications

References 57 publications

Soil Mineral Composition and Salinity Are the Main Factors Regulating the Bacterial Community Associated with the Roots of Coastal Sand Dune Halophytes

Soil Mineral Composition and Salinity Are the Main Factors Regulating the Bacterial Community Associated with the Roots of Coastal Sand Dune Halophytes

The aminoshikimic acid pathway in bacteria as source of precursors for the synthesis of antibacterial and antiviral compounds

Microfluidic fabrication of photo-responsive Ansamitocin P-3 loaded liposomes for the treatment of breast cancer

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