Microfluidic fabrication of photo-responsive Ansamitocin P-3 loaded liposomes for the treatment of breast cancer

Abstract

Ansamitocin P-3 (AP-3) is a promising anticancer agent. However, its low solubility has limited its biomedical applications. On the other hand, the preparation of liposomal formulations for drug delivery using...

Microfluidic Formulation of Curcumin-Loaded Multiresponsive Gelatin Nanoparticles for Anticancer Therapy

“…The total flow rates of the two phases were set at 10.5 and 40 mL/min. The two phases were injected into the inlets of the microfluidic device ,,, for the preparation of gelatin NPs. The particle size and PDI of the gelatin NPs were recorded to optimize the experimental conditions.…”

“…On the other hand, the controlled release of anticancer drugs can be achieved through the fine-tuning of the chemical properties of constituent materials used in NP fabrication. At present, commonly used biomaterials for nanodrug formulations include lipids (e.g., liposomes, lipid nanoparticles), − polymers (e.g., PLGA, PLA, chitosan, alginate), and proteins (e.g., collagen, gelatin, silk, albumin). ,− Gelatin is a natural protein that can be obtained by partial acid or alkali hydrolysis, thermal degradation, or enzymatic degradation of structural animal collagen . Compared with collagen, gelatin does not express antigenicity in vivo and is easy to dissolve in an aqueous solution .…”

“…Microfluidics can provide precise control and rapid mixing and has shown great potential in developing new NPs with controllable sizes and compositions. ,, The commonly used microfluidic technologies include the T-junction, hydrodynamic flow focusing, staggered herringbone, toroidal mixer, multi-inlet vortex mixers, etc. , Recently, a newly developed swirl mixer has shown great potential for the fabrication of lipid NPs and polymer NPs at a large scale with high uniformity . It has been used for the formulation of silk NPs, , ZIF-8 NPs, curcumin, doxorubicin, and Ansamitocin P-3 loaded liposomes for anticancer therapy. The swirl mixer can achieve a high flow rate of 320 mL/min with a mixing time of 0.1 ms, achieving a PDI of 0.06 ± 0.02 for liposome formulation, demonstrating its great potential for industrial application of nanoformulation.…”

Expert‐in‐the‐loop design of integral nuclear data experiments

“…Automation and AI methods are driving experimental science, particularly the discovery of new chemicals [13], genes [31], and materials [17], by enabling high throughput experiments. Beyond just executing the experiment, an AI algorithm can guide the experimental process sequentially by learning from the experiments it is performing.…”

Automated odor-blending with one-pot Bayesian optimization

“…This would enable humans to understand these hazardous odors and dramatically reduce the number of accidents based on hazardous odors such as natural gas leaked from pipelines 5 and volcanic gases. 6 To establish an automated odor-blending system, we need to develop three elements: an olfactory sensor [7][8][9][10][11][12][13] to replace the human sense of odors, blackbox optimization [14][15][16] to determine the amount of blending, and a robotic system [17][18][19][20][21][22][23][24][25] to perform actual blending of samples.…”