Metabolomic analysis of honey bee, Apis mellifera L. response to thiacloprid

Shi, Tengfei; Burton, Sawyer; Wang, Yufei; Xu, Shengyun; Zhang, Wenxin; Yu, Linsheng

doi:10.1016/j.pestbp.2018.08.003

Cited by 38 publications

(23 citation statements)

References 62 publications

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“…Sanchez‐Bayo and Goka believe that toxicity is of the utmost importance when considering the three factors of residue loads, prevalence and toxicity . In recent years, some studies of the transcriptome and metabolome based on the chronic toxicity of insecticides have been carried out in adult bees, and observed effects have included altering detoxification gene expression pathways, affecting components of the cellular response in the immune system, downregulating genes involved in learning performance in the brain, increasing the abundance of metabolites associated with oxidative stress and detoxification, damaging DNA, reducing antioxidant enzyme activity, and inducing transcriptional effects in the brains of bees . Future research is needed to analyze the effects of insecticides on the transcriptome and metabolome of honey bee larvae based on chronic toxicity.…”

Section: Discussionmentioning

confidence: 99%

Acute and chronic toxicity of acetamiprid, carbaryl, cypermethrin and deltamethrin to Apis mellifera larvae reared in vitro

Yang

Liu³

et al. 2019

Pest Management Science

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Background The effects of exposing Apis mellifera larvae to common insecticides were tested in the laboratory. Results The acute toxicity values of the four insecticides that we tested ranged from high toxicity to low toxicity: deltamethrin > cypermethrin > carbaryl > acetamiprid. The NOAEC (no observed adverse effect concentration) values of the chronic toxicity tests for each compound are 5 mg L−1 for acetamiprid, 2 mg L−1 for carbaryl, 1 mg L−1 for cypermethrin, and 0.2 mg L−1 for deltamethrin. Conclusion According to the risk quotient (RQ) values of acute and chronic toxicity that we obtained, the risk is acceptable at exposure rates that have been identified in the field. Overall, our results are valuable for evaluating the acute and chronic toxicities of these insecticides to developing honey bees. © 2019 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 99%

Acute and chronic toxicity of acetamiprid, carbaryl, cypermethrin and deltamethrin to Apis mellifera larvae reared in vitro

Yang

Liu³

et al. 2019

Pest Management Science

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“…Phylogenetic analysis revealed a close relationship between P450 genes from M. pulchricornis, A. mellifera , D. melanogaster, and N. vitripennis . The homology between AmelCYP4G11 and MpulCYP4G283 was 100%, and AmelCYP4G11 may play an important role in the hygiene behavior and antioxidant process of A. mellifera [ 34 ]. Therefore, it was speculated that MpulCYP4G283 may play the same role in M. pulchricornis .…”

Section: Discussionmentioning

confidence: 99%

Cytochrome P450s Are Essential for Insecticide Tolerance in the Endoparasitoid Wasp Meteorus pulchricornis (Hymenoptera: Braconidae)

Xing

Yan

Pang

et al. 2021

Insects

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With the widespread application of insecticides, parasitoid wasps may also be under risk when exposed to insecticides directly at their free-living stages. The endoparasitoid wasp Meteorus pulchricornis is the predominant natural enemy of many lepidopteran pests, such as Spodoptera litura and Helicoverpa armigera. The cytochrome P450 monooxygenases constitute a ubiquitous and complex superfamily of hydrophobic, haem-containing enzymes. P450s are involved in the detoxification of many xenobiotics. However, their exact roles in the tolerance mechanism in parasitoids toward insecticides has received less attention. Here, 28 P450 genes in M. pulchricornis were identified from a previously constructed transcriptome dataset. These P450 genes belonged to CYP2, -3, and -4, and mitochondrial clans. Subsequently, eight candidate MpulCYPs were selected from four CYP clans to validate their expression patterns under phoxim, cypermethrin, and chlorfenapyr exposure by qRT-PCR. The results showed that all three insecticides had significant effects on the expression of MpulCYPs. To further study the function of P450s, CYP369B3 was silenced, and its expression levels of CYP369B3 were significantly decreased. Survival analysis indicated that after dsRNA injection, the mortality rate of wasps was significantly increased when M. pulchricornis females were exposed to insecticides compared to control groups. Our findings provide a theoretical base for elucidating the mechanism of insecticide tolerance and promote functional research on P450 genes in parasitoid wasps.

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“…Botanical pesticides and heavy metal ions can damage the nervous system, inhibit or stimulate the excitation of dopamine neurons, 1 hinder the secretion and conduction of dopamine, 2 and reduce the expression of memory genes and proteins. 3,4 Azadirachtin, an insecticidal active component of Azadirachta indica, has attracted considerable attention both locally and internationally. 5,6 However, it is a broad-spectrum compound exhibiting a potentially low level of selectivity to non-targeted arthropods, including some pollinator species.…”

Section: Introductionmentioning

confidence: 99%

“…The influence of pesticides on cognitive behavior has attracted increasing attention. Botanical pesticides and heavy metal ions can damage the nervous system, inhibit or stimulate the excitation of dopamine neurons, 1 hinder the secretion and conduction of dopamine, 2 and reduce the expression of memory genes and proteins 3,4 . Azadirachtin, an insecticidal active component of Azadirachta indica , has attracted considerable attention both locally and internationally 5,6 .…”

Section: Introductionmentioning

confidence: 99%

Azadirachtin downregulates the expression of the CREB gene and protein in the brain and directly or indirectly affects the cognitive behavior of the Spodoptera litura fourth‐instar larvae

Qin

You

Zhang

et al. 2021

Pest Management Science

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BACKGROUND Azadirachtin has the potential to be used for pest control. Nevertheless, few studies have investigated the effects of azadirachtin on the cognitive behavior of pests. In this study, expression of the cAMP response element‐binding protein (CREB) and its gene were studied via a series of experiments in Spodoptera litura larvae treated with azadirachtin. RESULTS RNA‐Seq analysis of S. litura larvae treated with azadirachtin was undertaken. According to Kyoto Encyclopedia of Genes and Genomes analysis, the top 20 enriched pathways included neuroactive ligand–receptor interaction pathways, with seven significantly differentially expressed genes including CREB. Quantitative real time polymerase chain reaction (qRT‐PCR) results indicated that the CREB gene was expressed during all developmental stages of S. litura, but relative expression of the CREB gene was significantly downregulated after treatment with azadirachtin. Grayscale statistical analysis also showed that expression levels of protein kinase A (PKA), extracellular signal‐regulated kinase (ERK) and CREB proteins were significantly downregulated after treatment with azadirachtin. Moreover, RNA interference results showed that the effect of azadirachtin on the cognitive behavior of S. litura was consistent with that seen after interfering with CREB. In addition, larval selectivity to addictive odor sources was reduced, and the initial reaction time was increased. CONCLUSIONS This study clarified that azadirachtin can affect the cognitive behavior of S. litura and treatment with azadirachtin resulted in a downregulation of gene and protein expression of CREB and its pathway proteins. © 2020 Society of Chemical Industry

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Metabolomic analysis of honey bee, Apis mellifera L. response to thiacloprid

Cited by 38 publications

References 62 publications

Acute and chronic toxicity of acetamiprid, carbaryl, cypermethrin and deltamethrin to Apis mellifera larvae reared in vitro

Acute and chronic toxicity of acetamiprid, carbaryl, cypermethrin and deltamethrin to Apis mellifera larvae reared in vitro

Cytochrome P450s Are Essential for Insecticide Tolerance in the Endoparasitoid Wasp Meteorus pulchricornis (Hymenoptera: Braconidae)

Azadirachtin downregulates the expression of the CREB gene and protein in the brain and directly or indirectly affects the cognitive behavior of the Spodoptera litura fourth‐instar larvae

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