Azadirachtin downregulates the expression of the <scp>CREB</scp> gene and protein in the brain and directly or indirectly affects the cognitive behavior of the <i>Spodoptera litura</i> fourth‐instar larvae

Qin, Deqiang; You, Zhou; Zhang, Peiwen; Liu, Benju; Zheng, Qun; Zhang, Zhixiang

doi:10.1002/ps.6212

Cited by 7 publications

(5 citation statements)

References 67 publications

(108 reference statements)

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“…CREB plays important roles in a wide range of cellular processes, including proliferation, differentiation, and adaptive responses, by regulating glycogen and lipid metabolism in mammals [ 48 ]. In insects, CREB was reported to function in regulating metamorphosis, metabolism, learning and memory [ 12 , 50 , 56 , 57 ]. In addition, AKH can activate CREB through protein kinase A ( PKA )‐mediated phosphorylation [ 58 , 59 ].…”

Section: Discussionmentioning

confidence: 99%

MiR-2b-2-5p regulates lipid metabolism and reproduction by targeting CREB in Bactrocera dorsalis

et al. 2023

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In female animals, metabolic homoeostasis and reproductive fitness are critical to population expansion. The trade-off between lipid storage and reproduction inevitably occurs. However, most studies have focused on the complex network of relationships between reproductive and metabolic physiology at the transcriptional level. In this study, we identified a microRNA, miR-2b-2-5p, in a highly invasive quarantine pest, Bactrocera dorsalis . Knockdown of miR-2b-2-5p by antagomiR microinjection impaired ovarian development, reduced fecundity, and decreased triglyceride (TAG) storage in the fat body, whereas overexpression of miR-2b-2-5p by injection of its mimic caused reproductive defects similar to knockdown but increased TAG. Bioinformatics analysis and dual luciferase assay indicated that cyclic AMP response element (CRE)-binding protein (CREB) was the target gene of miR-2b-2-5p. RNAi-mediated knockdown of CREB led to excessive lipid storage and reproductive defects. Further starvation treatment revealed that miR-2b-2-5p functions by fine-tuning CREB expression in response to dietary stimuli. These results suggest that miR-2b-2-5p acts as a monitor to regulate CREB mRNA levels in the fat body, maintaining lipid homoeostasis and keeping the reproductive system on track. Thus, our study not only provides new insights into the interaction between metabolism and reproduction at the posttranscriptional level in B. dorsalis , but also providing a potential eco-friendly control strategy (RNAi-based biopesticides targeting essential miRNAs) for this notorious agricultural pest.

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Section: Discussionmentioning

confidence: 99%

MiR-2b-2-5p regulates lipid metabolism and reproduction by targeting CREB in Bactrocera dorsalis

et al. 2023

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“…The growth rate of fresh weight of the third instars treated with L. dispar solution was lower than that of distilled water and DMSO solution treatment for 3 consecutive days, indicating that L. dispar could inhibit the growth of diamondback moth larvae [61] . Azadirachtin is the main insecticidal component in neem tree, and it belongs to the tetranortriterpenoids [178,179] . Research confirmed that azadirachtin inhibited insect eggs from hatching and also affected the newborn larvae of treated eggs.…”

Section: Growth Inhibitionmentioning

confidence: 99%

Quantitative Study on Anti-Pest Activity of Natural Products Based on Visualization Framework of Knowledge Graph

2023

Front. Agr. Sci. Eng.

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“…It is a broad-spectrum, low-toxicity, and easily degradable insecticide recognised worldwide. It has repellent and anti-feeding effects on many insects (Lin et al 2021;Qin et al 2021). At present, the control of S.frugiperda is still mainly dependent on chemical control, and resistance has become a major problem.…”

Section: Introductionmentioning

confidence: 99%

“…It has repellent and anti-feeding effects on many insects. 16 Azadirachtin has been reported to induce antifeeding, growth and development inhibition effects in S. frugiperda. 17,18 However, the effects of azadirachtin exposure on the silk gland has not been reported yet.…”

Section: Introductionmentioning

confidence: 99%

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Azadirachtin inhibits the development and metabolism of the silk glands of Spodoptera frugiperda and affects spinning behavior

Zhao

Zheng

Qin

et al. 2022

Pest Management Science

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Spodoptera frugiperda is a major agricultural pest, and the 1st -3rd instar S. frugiperda have spinning behaviour and can drift to adjacent plants with the help of the wind. The present study found that azadirachtin can affect this behaviour, and the mechanism by which azadirachtin affects the spinning behaviour of S. frugiperda has been studied. In this study, the 3rd instar larvae were exposed to azadirachtin, and the pathological changes in the silk glands of S. frugiperda and the differences in their metabolites were analysed by pathological sectioning, transmission electron microscopy, metabolomics and other experimental methods. The results showed that azadirachtin can affect the silk gland of S. frugiperda. After 48 h of treatment with azadirachtin, the silk gland lumen of S. frugiperda appeared vacuolated, and KEGG showed that 31 different metabolites were identi ed, of which 12 were upregulated and 19 were downregulated. These metabolites were enriched in 15 different metabolic pathways, which pathways indicated that the silk gland of S. frugiperda was closely related to the formation of fatty acids and to energy metabolism in the process of silk formation. This research can further explain the spinning mechanism of insects and provide a research basis for determining the spinning, cocooning, and transfer damage mechanisms of many agricultural and forestry pests. Key MessageThe 1st -3rd instar Spodoptera frugiperda can spit and drift to neighbouring plants.Azadirachtin causes a decrease in the amino acids histidine, glycine, and leucine in the silk glands of Spodoptera frugiperda.Azadirachtin causes insect silk to break more easily.Feeding with azadirachtin for 72 h reduces Spodoptera frugiperda spinning.Azadirachtin can affect the development of the silk glands of Spodoptera frugiperda and thus affect silking behaviour.

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Azadirachtin downregulates the expression of the CREB gene and protein in the brain and directly or indirectly affects the cognitive behavior of the Spodoptera litura fourth‐instar larvae

Cited by 7 publications

References 67 publications

MiR-2b-2-5p regulates lipid metabolism and reproduction by targeting CREB in Bactrocera dorsalis

MiR-2b-2-5p regulates lipid metabolism and reproduction by targeting CREB in Bactrocera dorsalis

Quantitative Study on Anti-Pest Activity of Natural Products Based on Visualization Framework of Knowledge Graph

Azadirachtin inhibits the development and metabolism of the silk glands of Spodoptera frugiperda and affects spinning behavior

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