Metabolite profiling, histological and oxidative stress responses in the grey mullet, Mugil cephalus exposed to the environmentally relevant concentrations of the heavy metal, Pb (NO3)2

Hajirezaee, Saeed; Ajdari, Ashkan; Azhang, Bizhan

doi:10.1016/j.cbpc.2021.109004

Cited by 7 publications

(7 citation statements)

References 67 publications

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“…4 and 5). Results are in accordance with findings from literature, as these types of histological changes in the liver are assessed to be a response of its metabolic function to the exposure of a polluted environment (Ben Ameur et al 2012;Gad El-Hak et al 2021;Hajirezaee et al 2021;Rajeshkumar et al 2015;Yacoub and Abdel Satar 2003). Lipid vacuolization was also found in several individuals from the fishpond of S'Ena Arrubia, but it was mainly present in the form of micro-steatosis, while macro-steatosis and foci of steatosis more frequently occurred in samples from the port (Fig.…”

Section: Histopathological Analysessupporting

confidence: 90%

The EU Interreg Project “GEREMIA” on waste management for the improvement of port waters: results on monitoring the health status of fish as bioindicator

Reboa

Besio

Cutroneo

et al. 2023

Environ Sci Pollut Res

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Highly anthropized areas as ports represent complex scenarios that require accurate monitoring plans aimed to address the environmental status. In this context, the activities of the EU Interreg Project “GEstione dei REflui per il MIglioramento delle Acque portuali (GEREMIA)” were focused on comparing sites differently affected by human presence, as the Port of Genoa and the natural area of the S’Ena Arrubia fishpond: a panel of analyses was carried out on Mugilidae fish sampled in these two areas, aimed to address trace metal accumulation in the liver, gills, and muscle, as well as cytochrome P450 (CYP450) induction in liver and biliary polycyclic aromatic hydrocarbon (PAH) metabolites, and histopathological alterations in the liver and gills. Chemical analyses in the liver, gills, and muscle of specimens collected in the port area showed an overall higher degree of trace metal contamination compared to the natural fishpond, and similar results were obtained in terms of CYP450 induction and biliary PAH metabolites, suggesting a higher exposure to organic compounds. In addition, histopathological analyses revealed a significant alteration and then a loss of functionality of liver and gill tissue in individuals from the port. Overall, this study describes the complex environmental pollution scenario in the Port of Genoa, confirming the importance of using multidisciplinary approaches and different types of analyses to address both the presence and the effects of contaminants in marine environments.

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Section: Histopathological Analysessupporting

confidence: 90%

The EU Interreg Project “GEREMIA” on waste management for the improvement of port waters: results on monitoring the health status of fish as bioindicator

Reboa

Besio

Cutroneo

et al. 2023

Environ Sci Pollut Res

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“…The findings of the current study indicated that SIE at a rate of 1400-2400mg/kg diet might enhance antioxidant defense and protect hepatocytes against toxic effects of cadmium. Key words: Heavy metal, liver, blood, herbal extract, fish Contamination of aquatic ecosystems with heavy metals and their effects on aquatic organisms has been the subject of many studies (Ansari et al, 2004;Bhuyan and Islam, 2017;Sodango et al, 2018;Joksimović et al, 2020;Hajirezaee et al, 2021).Heavy metals usually enter aquatic environments through effluents from industrial and agricultural activities (Tahar and Keltoum, 2011;Miskowiec et al, 2015;Yang et al, 2018).…”

mentioning

confidence: 99%

Dietary silymarin, Silybum marianum extract ameliorates cadmium chloride toxicity in common carp, Cyprinus carpio

Al‐Shawi

YOUSIF

Al-Younis

et al. 2022

Annals of Animal Science

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The present study evaluated the protective effects of silymarin extract (SIE) on cadmium chloride toxicity in common carp, Cyprinus carpio. Four experimental group were considered for the experiment including: SIE0 (control): non-SIE-supplemented fish, SIE1: fish supplemented with 400 mg SIE/kg diet, SIE2: fish supplemented with1400mg SIE/kg diet, SIE3: fish supplemented with 2400 mg SIE/kg diet). Fish were fed experimental diet for 60 days and then exposed to cadmium chloride (1.5 mg/l or 25% of LC50–96 h) and antioxidant defense components and the survival rate assayed. After 60 days feeding trial, total antioxidant capacity (TAC) levels significantly increased (P<0.01) in 1400–2400 mg SIE/kg diet treatments compared to those in control and 400 mg SIE/kg diet treatment. Malondialdehyde (MDA) (P>0.01) and acetylcholinesterase (AChE) levels (P>0.01) remained unchanged during the feeding period in all treatments. Hepatic catalase (CAT) in all SIE supplemented groups and superoxide dismutase (SOD) and glutathione peroxidase (GPx) in 1400–2400 mg SIE/kg diet treatments significantly elevated (P<0.01) in response to SIE. Plasma levels of hepatic metabolic enzymes [alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), creatine kinase (CK), lactate dehydrogenase (LDH) ] remained unchanged (P>0.01) in all experimental groups over feeding period. After exposure to cadmium chloride, TAC levels were significantly elevated (P<0.01) in all experimental groups. In control and 400 mg SIE/kg diet treatment, TAC showed lower levels (P<0.01) compared to other groups. MDA levels were significantly increased (P<0.01) in control and fish supplemented with 400 and 1400 mg SIE/kg diet. TAC in the fish of 2400 mg SIE/kg diet treatment remained unchanged (P>0.01), following the exposure. CAT (P<0.01), SOD (P<0.01) and GPx (P<0.01) were significantly elevated in response to cadmium chloride in all groups. However, the treatments, 1400 and 2400 mg SIE/kg diet, showed lower increases (P<0.01) of enzymes. AChE activity (P<0.01) in the liver were significantly decreased in control and fish fed 400 and 1400 mg SIE/kg diet. Exposure to cadmium significantly increased (P<0.01) the plasma levels of ALT, AST, ALP and LDH in control and those fed 400 and 1400 mg SIE/kg diet. The findings of the current study indicated that SIE at a rate of 1400–2400mg/kg diet might enhance antioxidant defense and protect hepatocytes against toxic effects of cadmium.

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“…In addition, [35] pointed out in their study that the exposure of the common carp to Cd significantly increased the levels of MDA in the liver tissue (Table 10). Hajirezaee et al [38] registered increased MDA level in the plasma collected from Mugil cephalus individuals exposed to Pb compared to the ones in the control group. This is similar to our results, since the sampled tissues from fish collected from Danube Delta registered the highest MDA levels and Cd concentration in the muscle of these fish was above the maximum permitted level by the EU regulation.…”

Section: Non-linear Models Based On Random Forest (Rf) Algorithmmentioning

confidence: 97%

“…Several studies have conducted research linking the oxidative stress in fish liver and intestine [10,12,14,21,26,[29][30][31][32][33][34][35][36], in fish blood plasma [20,37,38], in fish muscle [21,32], in fish gill, kidney and brain [29][30][31]39] to heavy metals presence in the aquatic environment, proving that fish OS biomarkers can be used to determine heavy metal pollution since the trace element contamination directly affects fish welfare status.…”

Section: Introductionmentioning

confidence: 99%

Predictive Innovative Methods for Aquatic Heavy Metals Pollution Based on Bioindicators in Support of Blue Economy in the Danube River Basin

et al. 2021

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Heavy metal pollution is still present in the Danube River basin, due to intensive naval and agricultural activities conducted in the area. Therefore, continuous monitoring of this pivotal aquatic macro-system is necessary, through the development and optimization of monitoring methodologies. The main objective of the present study was to develop a prediction model for heavy metals accumulation in biological tissues, based on field gathered data which uses bioindicators (fish) and oxidative stress (OS) biomarkers. Samples of water and fish were collected from the lower sector of Danube River (DR), Danube Delta (DD) and Black Sea (BS). The following indicators were analyzed in samples: cadmium (Cd), lead (Pb), iron (Fe), zinc (Zn), copper (Cu) (in water and fish tissues), respectively, catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), malondialdehyde (MDA) (in fish tissues). The pollution index (PI) was calculated to identify the most polluted studied ecosystem, which revealed that Danube River is seriously affected by the presence of Fe (IP = 4887) and strongly affected by the presence of Zn (IP = 4.49). The concentration of Cd in fish muscle tissue was above the maximum permitted level (0.05 µg/g) by the EU regulation. From all analyzed OS biomarkers, MDA registered the highest median values in fish muscle (145.7 nmol/mg protein in DR, 201.03 nmol/mg protein in DD, 148.58 nmol/mg protein in BS) and fish liver (200.28 nmol/mg protein in DR, 163.67 nmol/mg protein, 158.51 nmol/mg protein), compared to CAT, SOD and GPx. The prediction of Cd, Pb, Zn, Fe and Cu in fish hepatic and muscle tissue was determined based on CAT, SOD, GPx and MDA, by using non-linear tree-based RF prediction models. The analysis emphasizes that MDA in hepatic tissue is the most important independent variable for predicting heavy metals in fish muscle and tissues at BS coast, followed by GPx in both hepatic and muscle tissues. The RF analytical framework revealed that CAT in muscle tissue, respectively, MDA and GPx in hepatic tissues are most common predictors for determining the heavy metals concentration in both muscle and hepatic tissues in DD area. For DR, the MDA in muscle, followed by MDA in hepatic tissue are the main predictors in RF analysis.

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Metabolite profiling, histological and oxidative stress responses in the grey mullet, Mugil cephalus exposed to the environmentally relevant concentrations of the heavy metal, Pb (NO3)2

Cited by 7 publications

References 67 publications

The EU Interreg Project “GEREMIA” on waste management for the improvement of port waters: results on monitoring the health status of fish as bioindicator

The EU Interreg Project “GEREMIA” on waste management for the improvement of port waters: results on monitoring the health status of fish as bioindicator

Dietary silymarin, Silybum marianum extract ameliorates cadmium chloride toxicity in common carp, Cyprinus carpio

Predictive Innovative Methods for Aquatic Heavy Metals Pollution Based on Bioindicators in Support of Blue Economy in the Danube River Basin

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