To address the rapidly growing use of probiotics in animal agriculture, this review discusses the effect of probiotics on animal growth and development, immune response, and productivity. Several benefits have been associated with the use of probiotics in farm animals, such as improved growth and feed efficiency, reduced mortality, and enhanced product quality. While the mechanisms through which probiotics induce their beneficial effects are not well understood, their role in modifying the gastrointestinal microbiota is believed to be the main mechanism. The use of probiotics in fresh and fermented meat products has been also shown to reduce pathogenic and spoilage microorganisms and improve sensory characteristics. Although many benefits have been associated with the use of probiotics, their effectiveness in improving animal performance and product quality is highly variable. Factors that dictate such variability are dependent on the probiotic strain being utilized and its stability during storage and administration/inoculation, frequency and dosage, nutritional and health status as well as age of the host animal. Therefore, future research should focus on finding more effective probiotic strains for the desired use and identifying the optimum dose, administration time, delivery method, and mechanism of action for each strain/host.

This study evaluated the effects of adding alcoholic and aqueous extracts of thyme on microbiological, chemical and sensory characteristics of yogurt. Supplementation of yogurt with thyme extracts during storage time were found to influence on yogurt composition compared to plain yogurt. So, plain yogurt (PY), thyme alcoholic extract yogurt (TCEY) and thyme aqueous extract yogurt (TAEY) moisture content decreased in all yogurt treatments to reach 88.21%, 88.39% and 88.23%, respectively after 28 days of storage. Protein content increased after 28 days of storage and reached to 4.82%, 4.83% and 4.81%, respectively. Fat content tended to increase at the end of study period to reach 0.32%, 0.36 and 0.326% respectively. The ash percentage after 28 days of storage increased to reach 0.71%, 0.72% and 0.71%, respectively. Titratable acidity increased after 28 day to 1.28, 1.17 and 1.2 while the pH decreased to 4.24, 4.31 and 4.29 respectively. For sensory evaluation, (TCEY) was more acceptable followed by (TAEY) and (PY) respectively. Microbial content revealed that all yogurt treatments were close at zero time for lactic acid bacteria (LAB) content and increased gradually to (7.38, 7.31 and 7.32) log (cfu/ml) respectively, there wasn't any grow during all study periods in (TCEY) and (TAEY) for coliform and yeast & mold content, while there was a grow in (PY) after 14 day for coliform and after 21 day for yeast & mold.

Green tea is high in polyphenols -compounds that have a variety of physiological functions. Microwave-assisted extraction (MAE) was used in this study to extract polyphenols from green tea. The MAE of the phenols in green tea was studied using an orthogonal configuration. As a result of UV/vis spectrophotometric methods, the total phenol content of tea infusions was determined. Polyphenols are plant-based chemicals that we acquire from specific foods. They may provide health advantages and are high in antioxidants. Polyphenols are considered to enhance or aid in the treatment of cardiovascular disease, neurodegenerative illness, diabetes, weight management concerns, and digestive problems. Each of the following factors has an impact on extraction: microwave intensity, microwave irradiate time, and frequency of microwave irradiation, as well as the tea/water ratio. Microwave radiation at 600 watts for 3 minutes at a frequency of once produced the best extraction results with a tea/water ratio of 1:20. As compared to traditional methods, MAE has a number of advantages. These include shorter extraction times, energy savings, and reduced environmental impact. A significant source of worry for food suppliers and users is the oxidation of dietary lipids. Antioxidant compounds have been used to prevent the oxidation of lipids. Synthetic antioxidant additions used today include C11H16O2 (BHA), C10H14O2 (TBHQ), and C15H24O (BHT). Toxicological and nutritional concerns, on the other hand, restrict their use. A variety of foods use green tea as a natural preservative because of its powerful antioxidant and antibacterial properties.

The present study evaluated the protective effects of silymarin extract (SIE) on cadmium chloride toxicity in common carp, Cyprinus carpio. Four experimental group were considered for the experiment including: SIE0 (control): non-SIE-supplemented fish, SIE1: fish supplemented with 400 mg SIE/kg diet, SIE2: fish supplemented with1400mg SIE/kg diet, SIE3: fish supplemented with 2400 mg SIE/kg diet). Fish were fed experimental diet for 60 days and then exposed to cadmium chloride (1.5 mg/l or 25% of LC50–96 h) and antioxidant defense components and the survival rate assayed. After 60 days feeding trial, total antioxidant capacity (TAC) levels significantly increased (P<0.01) in 1400–2400 mg SIE/kg diet treatments compared to those in control and 400 mg SIE/kg diet treatment. Malondialdehyde (MDA) (P>0.01) and acetylcholinesterase (AChE) levels (P>0.01) remained unchanged during the feeding period in all treatments. Hepatic catalase (CAT) in all SIE supplemented groups and superoxide dismutase (SOD) and glutathione peroxidase (GPx) in 1400–2400 mg SIE/kg diet treatments significantly elevated (P<0.01) in response to SIE. Plasma levels of hepatic metabolic enzymes [alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), creatine kinase (CK), lactate dehydrogenase (LDH) ] remained unchanged (P>0.01) in all experimental groups over feeding period. After exposure to cadmium chloride, TAC levels were significantly elevated (P<0.01) in all experimental groups. In control and 400 mg SIE/kg diet treatment, TAC showed lower levels (P<0.01) compared to other groups. MDA levels were significantly increased (P<0.01) in control and fish supplemented with 400 and 1400 mg SIE/kg diet. TAC in the fish of 2400 mg SIE/kg diet treatment remained unchanged (P>0.01), following the exposure. CAT (P<0.01), SOD (P<0.01) and GPx (P<0.01) were significantly elevated in response to cadmium chloride in all groups. However, the treatments, 1400 and 2400 mg SIE/kg diet, showed lower increases (P<0.01) of enzymes. AChE activity (P<0.01) in the liver were significantly decreased in control and fish fed 400 and 1400 mg SIE/kg diet. Exposure to cadmium significantly increased (P<0.01) the plasma levels of ALT, AST, ALP and LDH in control and those fed 400 and 1400 mg SIE/kg diet. The findings of the current study indicated that SIE at a rate of 1400–2400mg/kg diet might enhance antioxidant defense and protect hepatocytes against toxic effects of cadmium.

The present study aimed to Isolate trans-glutaminase EC:2.3.2.13 from some plants sources and Purified it and Studied it’s Charctarestics as well as it’s practical applications in the production of sausage.The enzyme was extracted from four types of plants (rosemary, chard, radish, arugula) using nine extraction solutions that included distilled water,Sodium chloride 3% solution, sodium chloride 5% solution, sodium phosphate solution 0.1 M and an pH 6.5, sodium phosphate 0.1 M and an pH of 7.5, Tris - Hcl solution 0.2 M and pH 7, Tris - Hcl solution 0.2 M and an pH 8, Tris - Hcl solution 0.1 M and an pH 7 and Tris - Hcl solution 0.1 M and an pH 8 in order to find out the best source of enzyme and the best extraction solution. chard was the best source of enzyme compared with other sources, Tirs -HCl 0.1M, pH 8 solution was the best extraction solution which gave the highest specific activity 8.104 unit/mg.Protein content for the crude enzyme extracts were concentrated using saturated ammonium sulfate in arrange 20-60%, Dialysis was done using distilled water. Then, the purification steps of the enzyme were completed using the gel filtration in the Sephadex G-100 Purification Folds 13.91 time and the yield was 20.04% %. Electrophoresise process using poly acryl amid gel in the absence of SDS observe the presence of one protein band which indicates the complete purification of transglutaminase. transglutaminase molecular weight was 42,660 Dalton when it was evaluated using poly acryl amide electrophoresis in the presence of SDS. the optimum pH for enzyme activity and enzyme stability was 7, while the optimum temperature for enzyme activity was 55°C, and the optimum temperature for enzyme stability was between 25-45°C.