Metabolism of the rabbit heartin-situ during asphyxia and post-asphyxial recovery

Isselhard, W.; M�urer, W.; Stremmel, W; Krebs, John S.; Schmitz, Heribert; Neuhof, Harold; Esser, Andrea; Ammermann, D.; Sunder-Plassmann, Ingrid; Schalenbach, Helga; Neu, Ursula

doi:10.1007/bf00586484

Cited by 46 publications

(12 citation statements)

References 49 publications

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“…Hearts, the ATP level of which had been reduced to 46%i of the control by a series of short asphyxiations of the rabbits and which averaged 72.5°/o after com pletion of the initial rapid phase of ATP-restoration by rephosphoryla tion of degradation products, exhibited a normalization of the tissue lev els of ATP and SAN within 21 h [12]. The rate of the postasphyxial in crease in the ATP level in this second phase of the ATP restoration by resynthesis of nucleotides amounted to about 0.06 pM/g wet tissue/h.…”

Section: Discussionmentioning

confidence: 99%

“…Since the tissue level of CrP is an inverse function of the myocardial 0 2-consumption [17] and thus of the work of the myocardium [6,18], it would be tempting to explain the increase in CrP by a temporary relief of the mechanical work of the ischemically stressed musculature. But this rather long-lasting supernor mal degree of phosphorylation of creatine -the ratio CrP/tCr rose from 0.4 under control conditions to 0.7 -was also found in whole hearts which recovered from asphyxia and which performed normal circulatory work [8,9,12]. Therefore it must be assumed that the increase in CrP under these conditions was not the consequence of a mechanical relief of the myocardium but resulted from a temporary shifting in intracellular equilibria which so far remains speculative.…”

Section: Discussionmentioning

confidence: 99%

“…The rate of changes is a function of the myocardial energy requirement [7,15,17], and during RCI it was slower than in hearts of asphyxiated animals [12], in anoxically perfused hearts [17], and in hearts during ischemia-induced cardiac arrest [17] under comparable conditions of anesthesia and temperature. In these situations the hearts perform either circulatory work or at least beat emptily for some time.…”

Section: Discussionmentioning

confidence: 99%

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Metabolic and Structural Recovery of Left Ventricular Canine Myocardium from Regional Complete Ischemia

Isselhard¹,

Lauterjung²,

Witte³

et al. 1975

Eur Surg Res

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The capacity for recovery of the normothermic left ventricular myocardium from a regional complete ischemia (RCI) was investigated using changes in the myocardial metabolic status (ATP, ADP, AMP, creatine phosphate (CrP), free creatine, glycogen, glucose, lactate) and alterations of the morphology as parameters. In dogs, an area of the anterior wall of the left ventricular myocardium was temporarily deprived completely of its blood supply by 5–7 overlapping ligatures extending into the heart cavity. The metabolites of the adenylic acid-CrP system returned to normal tissue levels after 30 and 60 min of RCI within 14 and 35 days of recovery, respectively; restoration averaged 82% after 100 min, 74% after 140 min, and 38% after 180 min of RCI after 5 weeks of recovery. At the same time glycogen amounted to 163% after 100 min, 114% after 140 min, and 65% after 180 min of RCI. The biochemical data correlated well with the structural changes in the affected myocardium, especially with the amount of de- and regenerating heart muscle cells. These obviously were functionally defect and were not comparable with normal structured and functioning heart muscle cells.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Metabolic and Structural Recovery of Left Ventricular Canine Myocardium from Regional Complete Ischemia

Isselhard¹,

Lauterjung²,

Witte³

et al. 1975

Eur Surg Res

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“…So the difference found in the content of adenine nucleotides depends on a differ ent preservation of ADP and especially AMP. The preservation of high amounts of adenine nucleotides during anaerobiosis is important, because the products of the breakdown of the nucleotides can pass the cellular membrane and then are available no longer for the restoration of ATP stores during postischemic recov ery (18,19). The rapid fall in the ATP content of cerebral cortex and medulla oblongata shows that in newborns as well as in adults anaerobic glycolysis is insufficient to meet the cerebral energy requirements even for a short period of ischemia.…”

Section: Fischer/lsselhardmentioning

confidence: 99%

Metabolic Patterns in Several Tissues of Newborn Rabbits during Ischemia

Jh¹,

Isselhard²

1975

Neonatology

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The metabolic states of various tissues of newborn rabbits were studied before and after periods of ischemia of 5–40 min. The contents of substances of the energy distributing adenylic acid-creatine phosphate system as well as glycogen, glucose and lactate were determined and the results are discussed in comparison with the well-known values from ischemic tissues of adult rabbits. The preservation of high energy phosphates as well as the rate of glycolytic energy production during the course of ischemia was quite identical in the myocardium of newborns and adults in contrast to the different ability of newborn and adult rabbits to maintain circulation in anaerobic conditions. In the central nervous system the ATP contents decreased to very low levels within a few minutes in both groups although the glycolytic energy production was rather different. But the larger amounts of adenine nucleotides present in the newborns at any time of ischemia indicate a better chance of postischemic recovery. In the livers and the kidneys of the newborns higher rates of glycolytic energy production led to better preservation of the energy-rich substances while in skeletal muscle and the lung only slight differences occurred between newborns and adults.

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“…Since the dephosphorylated nucleotide breakdown products can readily penetrate the myocardial cell membrane (7)(8)(9)(10), oxygen deficiency leads to a remarkable diminution in the intracellular levels of adenine nucleotides which can still be detected during subsequent periods of postanoxic recovery (11). Recently, we have demonstrated that de novo synthesis of adenine nucleotides is enhanced in the rat heart during recovery from oxygen deficiency (12).…”

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confidence: 99%

Effect of Beta-Adrenergic Stimulation on Myocardial Adenine Nucleotide Metabolism

Zimmer

Gerlach

1974

Circulation Research

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The effects of isoproterenol, propranolol, and compound D600 (o-isopropyl-«-[(Nmethyl-Af-homoveratryl)-Y-aminopropyl]-3,4,5-trimethoxyphenytacetonitrile) on myocardial adenine nucleotide metabolism were studied in rat hearts in situ. Isoproterenol in doses between 0.1 and 25 mg/kg induced an increase in heart rate concomitant with a significant acceleration in the de novo synthesis of adenine nucleotides (ATP, ADP, and AMP) and a diminution in their concentration. The effects of isoproterenol were antagonized by propranolol (1 and 50 mg/kg), which alone caused a reduction in the de novo synthesis of adenine nucleotides without inducing a change in their concentration. Compound D600 (10 mg/kg) brought about a slight elevation in the concentration of adenine nucleotides but did not influence the rate of de novo synthesis. The isoproterenol-induced diminution in adenine nucleotide concentration was prevented by D600; under these conditions, the acceleration of de novo synthesis was attenuated. These findings indicate that de novo synthesis of myocardial adenine nucleotides in the normal and the isoproterenolstimulated heart is regulated not only by a feedback mechanism dependent on the concentration of adenine nucleotides but also by j9-receptor-mediated alterations in carbohydrate metabolism which can cause changes in the size of the available pool of 5-phosphoribosyl-1-pyrophosphate.

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Metabolism of the rabbit heartin-situ during asphyxia and post-asphyxial recovery

Cited by 46 publications

References 49 publications

Metabolic and Structural Recovery of Left Ventricular Canine Myocardium from Regional Complete Ischemia

Metabolic and Structural Recovery of Left Ventricular Canine Myocardium from Regional Complete Ischemia

Metabolic Patterns in Several Tissues of Newborn Rabbits during Ischemia

Effect of Beta-Adrenergic Stimulation on Myocardial Adenine Nucleotide Metabolism

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