Metabolism of Spermatozoa

Salisbury, G. W.; Lodge, J. R.

doi:10.1002/9780470124888.ch2

Cited by 35 publications

(3 citation statements)

References 318 publications

(70 reference statements)

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“…This result is consistent with the findings of Iguer-Ouada and Verstegen (2001) and Tsutsui et al (2003) who reported that number of motile spermatozoa in chilled canine semen decreased gradually during storage at 4° C. This decrease in spermatozoa motility is due to initial increase of spermatozoa metabolism that increased uptake of oxygen and energy, and hence resulted in increased lactic acid concentration and decreased pH (Verstegen et al, 2005). Apart from that, osmotic pressure increases upon storage due to the accumulation of metabolic end products (Salisbury and Lodge, 1962). Songsasen et al (2002) reported that canine spermatozoa progressive motility was very sensitive to osmotic stress.…”

Section: Quality Of Chilled Semen In Extenders Supplemented With Sericinsupporting

confidence: 91%

Quality of Chilled Canine Semen in Tris-Egg Yolk Extender Supplemented With Sericin

Khye¹,

Yusuf²,

Satrio³

et al. 2021

J Kedokt Hewan

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The objective of this research was to evaluate the quality of chilled canine semen in Tris-egg yolk (TEY) extenders containing different concentrations of sericin. Semen were collected from four dogs by massage method. Canine semen was collected using sterile urine pots and evaluated. Sperm-rich fractions were pooled and divided into four equal aliquots, which were then diluted with TEY extenders supplemented with different concentrations of sericin (0%, 0.1%, 0.25%, 0.5%). The diluted semen aliquots were preserved at 4 ℃ in sterile centrifuge tubes and were then evaluated for spermatozoa motility, viability, plasma membrane integrity and acrosome integrity every 12 hours up to 72 h. The TEY extenders supplemented with 0.25% and 0.5% sericin resulted in higher spermatozoa motility and viability at 72 h compared to other TEY extenders (P0.05). The integrity of plasma membrane and acrosome of spermatozoa showed no significant differences among the groups extenders at 72 h. In conclusion, sericin in concentration of 0.25% and 0.5% were able to prevent the motility and viability of canine spermatozoa after storage for 72 h.

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Section: Quality Of Chilled Semen In Extenders Supplemented With Sericinsupporting

confidence: 91%

Quality of Chilled Canine Semen in Tris-Egg Yolk Extender Supplemented With Sericin

Khye¹,

Yusuf²,

Satrio³

et al. 2021

J Kedokt Hewan

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“…High temperatures (around 38C) increase sperm metabolism and shorten survival, whereas, at 4C, sperm are able to remain viable for a long period of time by halting their movements [14]. The epididymides of animals are normally anaerobic, have low pH and do not contain carbohydrates as a source of energy; therefore, respiration and glycolysis are suppressed in sperm, and they remain viable for approximately one month [11]. However, since blood flow stops and postmortem autolysis occurs after the death of a male animal or epididymis removal [2], sperm quality is considered to deteriorate markedly.…”

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confidence: 99%

Influence of the Time between Removal and Cooling of the Canine Epididymis on Post-Thaw Caudal Epididymal Sperm Quality

Hori

Uehara

Kawakami

et al. 2009

The Journal of Veterinary Medical Science

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ABSTRACT. The effects of the time period between canine epididymis removal and cooling on post-thaw caudal epididymal sperm quality were investigated. Sperm recovered from the epididymis stored for 6 hr at 4 or 20C exhibited similar motility. However, when the epididymis was stored for 12 hr or longer at 20C, sperm motility was significantly lower than that at 4C (p<0.01). The post-thawed qualities of sperm recovered from the caudal epididymides that had been stored at 20C for 0 or 6 hr and then at 4C for 24 hr after removal were not significantly different. Therefore, leaving the canine epididymis at 20C for up to 6 hr after its removal may have little effect on the post-thaw quality of recovered caudal epididymal sperm.KEY WORDS: canine, caudal epididymal sperm, frozen semen.J. Vet. Med. Sci. 71(6): 811-815, 2009 Many wild animals including canids are endangered. As artificial reproductive techniques to preserve these species, methods of gamete preservation have been investigated [1,5,7]. Gametes from male animals are generally collected by ejaculation. However, when animals die accidentally, sperm are recovered from the cauda epididymis, the sperm storage site [3,4,8].In a previous study [3], unilateral intrauterine insemination with sperm that was recovered from canine caudal epididymis in prostatic fluid (PF) and cryopreserved resulted in a high conception rate, 80.0%. We also showed that when caudal epididymal sperm were recovered after storage of the epididymis in sterile physiological saline at 4C for 48 hr, sperm motility was reduced, but intrauterine insemination with this post-thaw sperm again led to a high conception rate, 80.0% [4]. In these two experiments, the epididymis was kept at 4C immediately after removal, and the time period until the start of cooling after removal was not considered. However, when the bodies of male animals, such as wild animals, that have died accidentally are discovered, it is likely that several time have already passed under various temperature conditions, and so the epididymis can rarely be cooled immediately. This suggests that the time until cooling needs to be investigated.It has been reported that mouse caudal epididymal sperm shows a sperm motility of approximately 30% after 10 days of postmortem storage at 4C [6], but only shows a sperm motility of 10-15% after 24 hr of storage at room temperature [12]. Hishinuma et al. [2] reported that when the sika deer (Cervus nippon) epididymis is removed 4 hr postmortem and stored at 4C for 1-4 days, the caudal epididymal sperm motility is high, at approximately 50%, but, when removed 8-12 hr postmortem and stored at 4C for 1-4 days, the sperm motility is as low as 6.4%. These reports suggest that, in order to recover highly motile caudal epididymal sperm, it is important to store dead male animals immediately after death at 4C or to remove the epididymis as soon as possible and rapidly store it at 4C, thereby reducing the time of exposure to room temperature. However, in all the reported studies concerning...

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“…Spermatozoa motility has decreased as a result of an early increase in metabolism that consumes oxygen and energy and, in turn, caused an increase in lactic acid concentration and a drop in pH (Verstegen, Onclin, & Iguer-Ouada, 2005). In addition, Osmotic pressure rises during storage due to the accumulation of metabolic end products (Salisbury & Lodge, 1962). Also, progressive motility of canine spermatozoa was sensitive to osmotic stress (Songsasen et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

The Impact of Using Extenders Containing Low-density Lipoprotein Versus Egg Yolk with or without Ascorbic Acid on the Fertility Parameters of Post-thawed Chilled Canine Semen

Elbehiry,

Abd El-Aziz,

Belal

et al. 2024

Damanhour Journal of Veterinary Sciences

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Egg yolk is included in semen extenders because it prevents cold shock during chilling. This study aimed to assess the impact of substituting egg yolk with low-density lipoprotein (LDL) in Tris-Citric-Fructose (TCF) chilled extenders, with or without ascorbic acid, on the canine semen quality at 24, 48, and 72 hours of cooling. Semen from three German shepherd dogs was evaluated and extended at a 1:1 ratio using four extenders: Group A (TCF buffer, 20% egg yolk, with ascorbic acid), Group B (TCF buffer, 20% egg yolk without ascorbic acid), Group C (TCF buffer, 20% LDL, with ascorbic acid), and Group D (TCF buffer, 20% LDL without ascorbic acid). The extended semen was stored at 4°C and assessed after 24, 48, and 72 hours of cooling. The egg yolk extender resulted in less reduction in progressive sperm motility after 24, 48, and 72 hours of cooling; however, reduction in normal morphology, acrosome integrity, and cell membrane integrity were lower in TCF-LDL chilled extender than in TCF-EY chilled extender. Furthermore, after 24 hours of cooling, all evaluation parameters in both extenders were greater than 50%. In conclusion, the quality of chilled canine sperm was significantly improved by adding ascorbic acid to TCF-EY, but the improvement was only marginal when applied to TCF-LDL chilled extender.

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Metabolism of Spermatozoa

Cited by 35 publications

References 318 publications

Quality of Chilled Canine Semen in Tris-Egg Yolk Extender Supplemented With Sericin

Quality of Chilled Canine Semen in Tris-Egg Yolk Extender Supplemented With Sericin

Influence of the Time between Removal and Cooling of the Canine Epididymis on Post-Thaw Caudal Epididymal Sperm Quality

The Impact of Using Extenders Containing Low-density Lipoprotein Versus Egg Yolk with or without Ascorbic Acid on the Fertility Parameters of Post-thawed Chilled Canine Semen

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