Quality of Chilled Canine Semen in Tris-Egg Yolk Extender Supplemented With Sericin

Abstract

The objective of this research was to evaluate the quality of chilled canine semen in Tris-egg yolk (TEY) extenders containing different concentrations of sericin. Semen were collected from four dogs by massage method. Canine semen was collected using sterile urine pots and evaluated. Sperm-rich fractions were pooled and divided into four equal aliquots, which were then diluted with TEY extenders supplemented with different concentrations of sericin (0%, 0.1%, 0.25%, 0.5%). The diluted semen aliquots were preserved at 4 ℃ in sterile centrifuge tubes and were then evaluated for spermatozoa motility, viability, plasma membrane integrity and acrosome integrity every 12 hours up to 72 h. The TEY extenders supplemented with 0.25% and 0.5% sericin resulted in higher spermatozoa motility and viability at 72 h compared to other TEY extenders (P0.05). The integrity of plasma membrane and acrosome of spermatozoa showed no significant differences among the groups extenders at 72 h. In conclusion, sericin in concentration of 0.25% and 0.5% were able to prevent the motility and viability of canine spermatozoa after storage for 72 h.

The Impact of Using Extenders Containing Low-density Lipoprotein Versus Egg Yolk with or without Ascorbic Acid on the Fertility Parameters of Post-thawed Chilled Canine Semen

“…Our findings show that acrosome integrity, plasma membrane integrity and normal morphology percentages were reduced by 33, and 34 percent, respectively, in TCF-EY chilled semen at 24 and 48 hours. Our findings agree with those of Khye et al (2021) in terms of the deterioration of the overall quality of chilled semen with storage time, but the difference was in the rate of deterioration. It was faster in our investigation than in the study of Khye et al (2021).…”

“…Our findings agree with those of Khye et al (2021) in terms of the deterioration of the overall quality of chilled semen with storage time, but the difference was in the rate of deterioration. It was faster in our investigation than in the study of Khye et al (2021). They discovered a slight decrease in acrosome integrity (97.60.7 at 6 h to 95.01.5 at 24h to 93.22.2 percent at 48 h) and plasma membrane integrity (90.44.6 at 6h to 85.75.2 at 48 h).…”

Spermatozoa Quality of Kintamani Dogs in Coconut Water-Egg Yolk Diluent with Addition of Moringa Leaves and Carrot Extract

“…Furthermore, antibiotic penicillin 1000IU/mL (CAS 69-57-8 Sigma) and streptomycin 1mg/mL (CAS 3810-74-0 Sigma) diluents were mixed and then homogenized. The semen was diluted with a final concentration of 100x10 6 cells/mL (Romagnoli 2002;Khye et al 2021).…”

Effect of Spirulina and Coenzyme Q-10 Addition to Extenders on Canine Spermiogram

“…Anti-oxidants are chemical molecules or enzymes that neutralise ROS or react with ROS to create by-products that do not cause oxidation. Endogenously present antioxidant systems in spermatozoa and semen (glutathione peroxidase, superoxide dismutase, reduced glutathione, catalase, L-carnitine, acetyl carnitine, ascorbate, urate, glutathione, albumin and taurine [5], and exogenously added antioxidants (amino acids, vitamins, enzymes, sugars and antioxidant-rich substances) have been studied by various scientists [6][7][8][9][10][11][12][13][14][15][16], and the results have shown a positive effect of antioxidants on both chilled-extended and frozen-thawed semen. Two commonly used antioxidants that have been commonly added to semen extenders in other animals and birds, but have not yet been utilised in canine semen extenders, are Spirulina and coenzyme Q-10.…”