Metabolism of DDE, DDD, and DDT in sheep

Abstract

Sheep were dosed orally for 28 consecutive days with DDE, DDD, or DDT individually, or with a mixture of these three compounds. In a second similar trial DDE was administered either alone or simultaneously with DDD and/or DDT. The dose rate for each compound in all cases was 1 mg pp'-isomer/kg body weight/day. All sheep were sampled for blood and omental fat during the dosing period and the following year.Maximum concentrations determined in fat were in the order DDE > DDD > DDT in the approximate ratios 10: 2: 1. Rates of elimination from fat when dosing was stopped were in the order DDD >DDT >DDE, the half-life being 4, 9, and 14 weeks respectively. In fat, DDE appeared as a metabolite of DDD and DDT, and DDD as a metalobite of DDT. In the first trial the simultaneous administration of DDD and DDT with DDE appeared to stimulate the subsequent elimination of DDE from storage in fat, but this effect was not reproduced in the second trial. There was a correlation between DDE in fat (mg/kg = x) and DDE in whole blood (/Lg/kg = y) expressed by the regression equation.x = 1.8y-l.l A sheep was given one oral dose of DDE, DDD, and DDT simultaneously, each at the rate of 50 mg/kg body weight. Highest concentrations of DDT and DDE occurred in blood 15 hours and 32 hours respectively after dosing. DDD showed two maxima, at 8 hours and at 32 hours after dosing.The value of blood and fat analyses for diagnostic purposes is discussed in relation to the results of these trials.

Failure of ethanol and phenobarbitone to reduce organochlorine pesticide residues in sheep

“…The persistence of pp'DDE in these experiments confirms the results of Hunnego & Harrison (1971), who dosed sheep with pure pp'DDf and monitored fat levels for 52 weeks.…”

“…With the application of gas chromatography to the analysis of pesticides this metabolism has been reported in cattle (McCulley et al 1966;Fries & Kane 1967;Rumsey et al 1967) and sheep (Hunnego & Harrison 1971). …”

Application of Bayesian Population Physiologically Based Pharmacokinetic (PBPK) Modeling and Markov Chain Monte Carlo Simulations to Pesticide Kinetics Studies in Protected Marine Mammals: DDT, DDE, and DDD in Harbor Porpoises

“…In these studies, the pathways suggested are from DDT to DDD and from DDT to DDE. In sheep, however, DDE was suggested to appear as a metabolite of both DDT and DDD and this finding was confirmed later in rats. , In the past, DDXs have been found in marine mammal species, but the origin of DDD and DDE could not be determined. Given the above literature evidence, we believe that two major sources for DDD and DDE in harbor porpoises are through direct food intake and from biotransformation of DDT.…”

Enrichment and isolation of non-specific aromatic degraders from unique uncontaminated (plant and faecal material) sources and contaminated soils

“…1997 ). DDT has been detected at significant concentrations in the tissue of a number of animals ( Hunnego and Harrison 1971; Gilbert et al . 1990 ), and the organochlorine has also been reported to accumulate in grain, cotton and natural vegetation ( Voerman and Besemer 1975; Smelt et al .…”