Enrichment and isolation of non-specific aromatic degraders from unique uncontaminated (plant and faecal material) sources and contaminated soils

Juhasz, Albert L.; Naidu, Ravi

doi:10.1046/j.1365-2672.2000.01161.x

Cited by 22 publications

(10 citation statements)

References 25 publications

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“…strain SA-2. The ability of an enteric organism to grow with either PCBs or chlorobenzenes or both is an uncommon phenomenon, even though dechlorination of DDT in pure systems of E. aerogenes, E. cloacae and Escherichia coli has been described (Aislabie et al 1997;Juhasz and Naidu 2000). The well characterized and widely studied organisms, Alcaligenes eutrophus H850, Pseudomonas testosteroni H430, Corynebacterium sp.…”

Section: Discussionmentioning

confidence: 99%

Characterization of multiple novel aerobic polychlorinated biphenyl (PCB)-utilizing bacterial strains indigenous to contaminated tropical African soils

et al. 2007

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Contaminated sites in Lagos, Nigeria were screened for the presence of chlorobiphenyl-degrading bacteria. The technique of continual enrichment on Askarel fluid yielded bacterial isolates able to utilize dichlorobiphenyls (diCBs) as growth substrates and six were selected for further studies. Phenotypic typing and 16S rDNA analysis classified these organisms as species of Enterobacter, Ralstonia and Pseudomonas. All the strains readily utilized a broad spectrum of xenobiotics as sole sources of carbon and energy. Growth was observed on all monochlorobiphenyls (CBs), 2,2'-, 2,3-, 2,4'-, 3,3'- and 3,5-diCB as well as di- and trichlorobenzenes Growth was also sustainable on Askarel electrical transformer fluid and Aroclor 1221. Time-course studies using 100 ppm of 2-, 3- or 4-CB resulted in rapid exponential increases in cell numbers and CB transformation to respective chlorobenzoates (CBAs) within 70 h. Significant amounts of chloride were recovered in culture media of cells incubated with 2-CB and 3-CB, suggesting susceptibilities of both 2- and 3-chlorophenyl rings to attack, while the 4-CB was stoichiometrically transformed to 4-CBA. Extensive degradation of most of the congeners in Aroclor 1221 was observed when isolates were cultivated with the mixture as a sole carbon source. Aroclor 1221 was depleted by a minimum of 51% and maximum of 71%. Substantial amounts of chloride eliminated from the mixture ranged between 15 and 43%. These results suggest that some contaminated soils in the tropics may contain exotic micro-organisms whose abilities and potentials are previously unknown. An understanding of these novel strains therefore, may help answer questions about the microbial degradation of polychlorinated biphenyls (PCBs) in natural systems and enhance the potential use of bioremediation as an effective tool for cleanup of PCB-contaminated soils.

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Section: Discussionmentioning

confidence: 99%

Characterization of multiple novel aerobic polychlorinated biphenyl (PCB)-utilizing bacterial strains indigenous to contaminated tropical African soils

et al. 2007

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“…The ability of different strains of S. maltophilia to degrade xenobiotics under aerobic conditions is known (Schloter et al 2000). The list of degraded substances includes atrazine (Rousseaux et al 2001), pentachlorophenol (Juhasz and Naidu 2000), dimethylamine (Kroon and van Ginkel 2001) and hexahydro-1,3,5-trinitro-1,3,5-triozine (Bink et al 1995). The ability of S. maltophilia to use aniline, as a sole carbon source under oxic and anoxic conditions, has also been reported (Zissi and Lyberatos 1999).…”

Section: Irb For Phosphate Removal From Returned Liquormentioning

confidence: 99%

Phosphate removal from the returned liquor of municipal wastewater treatment plant using iron-reducing bacteria

Ivanov¹,

Stabnikov²,

Zhuang³

et al. 2005

J Appl Microbiol

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Aim:The application of iron-reducing bacteria (IRB) to phosphate removal from returned liquor (liquid fraction after activated sludge digestion and anaerobic sludge dewatering) of municipal wastewater treatment plant (WWTP) was studied. Methods and Results: An enrichment culture and two pure cultures of IRB, Stenotrophomonas maltophilia BK and Brachymonas denitrificans MK identified by 16S rRNA gene sequencing, were produced using returned liquor from a municipal WWTP as carbon and energy source, and iron hydroxide as oxidant. The final concentration of phosphate increased from 70 to 90 mg l )1 in the control and decreased from 70 to 1 mg l )1 in the experiment. The mass ratio of removed P to produced Fe(II) was 0AE17 g P g )1 Fe(II). The strain S. maltophilia BK showed the ability to reduce Fe(III) using such xenobiotics as diphenylamine, m-cresol, 2,4-dichlorphenol and p-phenylphenol as sole sources of carbon under anaerobic conditions. Conclusions: Bacterial reduction of ferric hydroxide enhanced the phosphate removal from the returned liquor. Significance and Impact of the Study: The ability of the facultative anaerobes S. maltophilia BK and B. denitrificans MK to reduce Fe(III) was shown. These micro-organisms can be used for anaerobic removal of phosphate and xenobiotics by bacterial reduction of ferric ions.

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“…Dechlorination of the 1,1,1-trichlor-2,2-bis(p-chlorophenyl)ethane (DDT) peticide to 1,1,-dichloro-2,2-bis(p-chlorophenyl)ethane (DDD) has been reported to occur in pure E. coli culture systems, (147), but the genes and proteins involved in this process are still unknown.…”

Section: Dehalogenation Reactionsmentioning

confidence: 99%

Biodegradation of Aromatic Compounds by Escherichia coli

Dı́az

Ferrández

Prieto

et al. 2001

Microbiol Mol Biol Rev

322

235

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Although Escherichia coli has long been recognized as the best-understood living organism, little was known about its abilities to use aromatic compounds as sole carbon and energy sources. This review gives an extensive overview of the current knowledge of the catabolism of aromatic compounds by E. coli. After giving a general overview of the aromatic compounds that E. coli strains encounter and mineralize in the different habitats that they colonize, we provide an up-to-date status report on the genes and proteins involved in the catabolism of such compounds, namely, several aromatic acids (phenylacetic acid, 3- and 4-hydroxyphenylacetic acid, phenylpropionic acid, 3-hydroxyphenylpropionic acid, and 3-hydroxycinnamic acid) and amines (phenylethylamine, tyramine, and dopamine). Other enzymatic activities acting on aromatic compounds in E. coli are also reviewed and evaluated. The review also reflects the present impact of genomic research and how the analysis of the whole E. coli genome reveals novel aromatic catabolic functions. Moreover, evolutionary considerations derived from sequence comparisons between the aromatic catabolic clusters of E. coli and homologous clusters from an increasing number of bacteria are also discussed. The recent progress in the understanding of the fundamentals that govern the degradation of aromatic compounds in E. coli makes this bacterium a very useful model system to decipher biochemical, genetic, evolutionary, and ecological aspects of the catabolism of such compounds. In the last part of the review, we discuss strategies and concepts to metabolically engineer E. coli to suit specific needs for biodegradation and biotransformation of aromatics and we provide several examples based on selected studies. Finally, conclusions derived from this review may serve as a lead for future research and applications

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Enrichment and isolation of non-specific aromatic degraders from unique uncontaminated (plant and faecal material) sources and contaminated soils

Cited by 22 publications

References 25 publications

Characterization of multiple novel aerobic polychlorinated biphenyl (PCB)-utilizing bacterial strains indigenous to contaminated tropical African soils

Characterization of multiple novel aerobic polychlorinated biphenyl (PCB)-utilizing bacterial strains indigenous to contaminated tropical African soils

Phosphate removal from the returned liquor of municipal wastewater treatment plant using iron-reducing bacteria

Biodegradation of Aromatic Compounds by Escherichia coli

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