Metabolic profiling of endophytic bacteria from Purwoceng (Pimpinella pruatjan Molkend) root and antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa

Purwestri, Yekti Asih; Kartikasari, Nur'aini; Putri, Sartika Gunawan; Wilson, Wildiani; Sembiring, Langkah

doi:10.1063/1.4953537

Cited by 6 publications

(5 citation statements)

References 13 publications

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“…All the isolates showed the presence of alkaloids (Figure 5A1-5A7). The presence of alkaloids in bacteria has also been reported in previous studies by Purwestri et al (2016), Munakata et al (2022), andPtak et al (2022). Alkaloid is a nitrogen base compound that poses numerous health benefits.…”

Section: Phytochemical Testsupporting

confidence: 63%

Isolation and characterization of endophytic bacteria from sterile leaf of Acrostichum aureum from Bengkalis Island (Riau, Indonesia) and its potency for antidiabetic

et al. 2023

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Abstract. Linda TM, Febriarti BL, Zul D, Sofyanti N, Berliansyah A, Delfira N, Devi S. 2023. Isolation and characterization of endophytic bacteria from sterile leaf of Acrostichum aureum from Bengkalis Island (Riau, Indonesia) and its potency for antidiabetic. Biodiversitas 24: 1580-1588. Endophytic bacteria are found in symbiotic plant tissues. These bacteria pose potential sources for the natural product due to their health benefit, e.g., for antidiabetic activity. The present study aimed to isolate and characterize endophytic bacteria isolates from the sterile leaf of sea fern (Acrostichum aureum Linn.) from Bengkalis Island (Riau Province), Indonesia. Furthermore, to investigate their potency as an antidiabetic agent. The characteristics of isolates bacteria were described based on colony morphology and biochemical tests. A total of six endophytic bacteria were isolated from the sterile leaf of Acrostichum aureum. Their characteristics vary in shape, color, and size. The phytochemical screening indicated the presence of alkaloids and saponin in all the isolates. The results exhibited that the extract of the sterile leaf of A. aureum also gave a similar phytochemical compound of isolate endophytic bacteria. The dendrogram constructed from morphological and biochemical characteristics showed the separation of D.SB 4.2 from other six bacteria strains due to the positive antibacterial activity for Escherichia coli ATCC35218, Staphylococcus aureus ATCC29213, Salmonella typhi ATCC14028, and Bacillus subtilis ATCC11774. Out of six isolates, only isolate D.SB 5.2 was selected for the antidiabetic test. The isolates showed positive results on the activity as a-amylase inhibitor (18.12%). This study provides important information on endophytic bacteria isolates on A. aureum and their potency for antidiabetic agents.

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Section: Phytochemical Testsupporting

confidence: 63%

Isolation and characterization of endophytic bacteria from sterile leaf of Acrostichum aureum from Bengkalis Island (Riau, Indonesia) and its potency for antidiabetic

et al. 2023

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“…Pada jaringan suatu tanaman obat terdapat setidaknya satu spesies mikroba endofit (Tan & Zou, 2001;Strobel, et al, 2004) Pseudomonas aeruginosa (Purwestri et. al., 2016).…”

Section: Pendahuluanunclassified

Kemampuan Antibakteri Metabolit Sekunder Bakteri Endofit Tanaman Purwoceng terhadap Escherechia coli

Kartikasari

Purwestri

2021

J. Ilmu Hayat

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Kajian tentang senyawa antibakteri yang dihasilkan oleh bakteri endofit mulai bermunculan untuk mengatasi meningkatnya bakteri resisten obat seperti Escherechia coli. Bakteri endofit (GP14, GP2 dan DG1) yang diisolasi dari purwoceng (Pimpinella pruatjan Molkend) telah diketahui memiliki potensin dalam mencegah pertumbuhan bakteri patogen. Pada penelitian ini isolat GP14, GP 2 dan DG 1 menunjukkan aktivitas untuk mencegah pertumbuhan E. coli. Senyawa bioaktif diekstraksi dari kultur cair menggunakan etil asetat, diikuti dengan uji Thin layer chromatography (TLC) sebagai uji profil metabolik untuk mengidentifikasi golongan senyawa kimia. Uji TLC menunjukkan bahwa isolat GP14, GP2 dan DG1 menghasilkan senyawa alkaloid. Uji aktivitas antibakteri menggunakan metode paper disc diffusion dengan media TSA. Konsentrasi yang digunakan adalah 25 mg/ml, 50 mg/ml, 100 mg/ml dan 200 mg/ml. Hasil penelitian menunjukkan bahwa ekstrak kasar hasil ekstraksi etil asetat memiliki aktivitas antibakteri terhadap E. coli. Zona hambat terbesar ditunjukkan dari ekstrak kasar isolat DG1 pada konsentrasi 200 mg/mL yaitu 2.135 mm.

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“…These bacteria have been known for years as the cause of serious infectious diseases such as eye, ear, chronic respiratory disease, pneumonia, urinary tract, blood vessels, and skin infections (Wu et al 2015). Compared to other bacteria P. aeruginosa has stronger resistance to the physical environment and chemicals (Umoh 2009;Retnaningrum and Wilopo 2018;Purwestri et al 2016).…”

Section: Introductionmentioning

confidence: 99%

The potency of Sansevieria trifasciata and S. cylindrica leaves extracts as an antibacterial against Pseudomonas aeruginosa

et al. 2021

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Abstract. Nugroho LH, Dewatisari WF, Retnaningrum E, Purwestri YA. 2021. The potency of Sansevieria trifasciata and S. cylindrica leaves extracts as an antibacterial against Pseudomonas aeruginosa. Biodiversitas 22: 408-415. Sansevieria trifasciata and Sansevieria cylindrica are the major herbs in Indonesia, which contain several bioactive compounds as potential sources of antibacterial agents. This study aims to evaluate the antibacterial activity of S. trifasciata and S. cylindrica leaves extract and fraction against Pseudomonas aeruginosa and to identify its bioactive compounds. Crude ethanolic extract of S. trifasciata and S. cylindrica leaves were tested for their antimicrobial activity by disk diffusion method against P. aeruginosa. S. trifasciata showed strong antibacterial activity with an inhibition zone of 18.3 mm compared to S. cylindrica. Different concentrations of extract i.e. 4 mg/mL, 8 mg/mL, 16 mg/mL, 32 mg/mL, 64 mg/mL, 128 mg/mL and 256 mg/mL were tested for their minimum inhibitory concentrations (MIC). The MIC results showed that the S. trifasciata extract was able to inhibit bacterial growth at a concentration of 32 mg/mL. Results of vacuum liquid chromatography (VLC) and thin-layer chromatography (TLC) revealed that only fraction 3 showed the highest antibacterial activity at 16 mg/mL. In TLC bioautography analysis, fraction 3 showed a clear zone at Rf 0.93. The phytochemical analysis showed that terpenoid, phenolic, triterpenoid, and flavonoid compounds were found in S. trifasciata extract that were associated with antibacterial activity.

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Metabolic profiling of endophytic bacteria from Purwoceng (Pimpinella pruatjan Molkend) root and antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa

Cited by 6 publications

References 13 publications

Isolation and characterization of endophytic bacteria from sterile leaf of Acrostichum aureum from Bengkalis Island (Riau, Indonesia) and its potency for antidiabetic

Isolation and characterization of endophytic bacteria from sterile leaf of Acrostichum aureum from Bengkalis Island (Riau, Indonesia) and its potency for antidiabetic

Kemampuan Antibakteri Metabolit Sekunder Bakteri Endofit Tanaman Purwoceng terhadap Escherechia coli

The potency of Sansevieria trifasciata and S. cylindrica leaves extracts as an antibacterial against Pseudomonas aeruginosa

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