The potency of Sansevieria trifasciata and S. cylindrica leaves extracts as an antibacterial against Pseudomonas aeruginosa

Dewatisari, Whika Febria; Nugroho, Laurentius Hartanto; Retnaningrum, Endah; Purwestri, Yekti Asih

doi:10.13057/biodiv/d220150

Cited by 16 publications

(14 citation statements)

References 24 publications

(32 reference statements)

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“…The obtained fraction was collected in an Erlenmeyer, poured into a porcelain dish and air-dried. VLC was followed by thin-layer chromatography to monitor the separation of the compound (8).…”

Section: Vacuum Chromatography-mass Spectrometrymentioning

confidence: 99%

Acute toxicity and anticancer potential of knobweed (Hyptis capitata) ethanolic leaf extract and fraction

2022

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Ethanolic leaf extract of Hyptis capitata Jacq. showed a toxic effect on Artemia salina larvae1. The extract was simplified through fractionation using vacuum liquid chromatography to trace compounds with biological activity. The F1, F2, F3 and F4 fractions were used in the acute toxicity test on A. salina larvae. F1 showed the highest toxicity value of 196.77±2.7 µg/ml. As an initial screening for its anticancer potential, ethanolic leaf extract and F1 fraction were subjected to cytotoxicity test on T47D cells to obtain an IC50 value of 45.32±2.0 and >1000 µg/mL. The LC50 of the F1 fraction and its cytotoxicity level decreased compared to the ethanolic leaf extract. Based on GC-MS analysis, the ethanolic leaf extract and the F1 fraction contained neophytadiene, hexadecanoic acid, methyl ester, hexadecanoic acid, ethyl ester and heptadecanoic acid, 16-methyl-, methyl ester. Meanwhile, ?-sitosterol, docosanoic acid, ethyl ester and squalene are only found in the ethanolic leaf extract. The compounds contained in ethanolic leaf extract work synergistically. This implies the ethanolic leaf extract has the potential to be developed as an anticancer candidate.

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Section: Vacuum Chromatography-mass Spectrometrymentioning

confidence: 99%

Acute toxicity and anticancer potential of knobweed (Hyptis capitata) ethanolic leaf extract and fraction

2022

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“…Dracaena (Sansevieria) trifasciata was selected based upon its previously reported ethnomedicinal significance [ 21 , 22 , 23 ]. Dracaena trifasciata was obtained from a plant nursery in Lahore.…”

Section: Methodsmentioning

confidence: 99%

Docking and Molecular Dynamics Study to Identify Novel Phytobiologics from Dracaena trifasciata against Metabolic Reprogramming in Rheumatoid Arthritis

Ahmed

John

Paracha

et al. 2022

Life

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Enhancement of glycolysis and glutaminolysis are the two most common modalities associated with metabolic reprogramming in rheumatoid arthritis (RA). This enhancement is concomitant to the upregulation of hexokinase 2 (HK2) and glutaminase 1 (GLS1). Hence, the current study was undertaken to identify potential phytobiological inhibitors against HK2 and GLS1, from Dracaena (Sansevieria) trifasciata, an indigenous ethnomedicinal plant found in Pakistan, using computational analysis. Phytobiologics from Dracaena trifasciata were assessed for their ability to co-inhibit HK2 and GLS1 via molecular docking and molecular dynamics simulations. The results underscored seven phytobiologics with promising binding affinities for both HK2 and GLS1. Molecular dynamics simulations further elucidated that all seven identified phytobiologics inhibited HK2 by forming stable complexes but only five amongst the seven had the potential to form stable complexes with GLS1 in real time, thereby implying the potential of co-inhibition for these five compounds. Compound 28MS exhibited an equally strong binding profile for both HK2 (−8.19 kcal/mol) and GLS1 (−8.99 kcal/mol). Furthermore, it exhibited a similar trend in stability during simulation for both targets. Our results serve as a primer for a more lucid understanding towards co-inhibition of HK2 and GLS1 using multiple computational approaches. The identified phytobiologics should undergo in-vitro and in-vivo validation to corroborate their therapeutic potential in RA.

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“…The mobile phase has eight compositions, namely a mixture of n-hexane:chloroform 3:1 (v/v), 2:2 (v/v), 1:3 (v/v), 100% chloroform, chloroform:methanol 3:1 (v /v), 2:2 (v/v),1:3 (v/v) and methanol 100%. The eluent obtained was accommodated in an Erlenmeyer flask followed by thin-layer chromatography (Dewatisari et al 2021).…”

Section: Fractionation With Vacuum Liquid Chromatography (Vlc)mentioning

confidence: 99%

Cytotoxicity extract and fraction of knobweed (Hyptis capitata) and its effect on migration and apoptosis of T47D cells

et al. 2022

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Abstract. To’bungan N, Pratiwi R, Widyarini S, Nugroho LH. 2021. Cytotoxicity extract and fraction of knobweed (Hyptis capitata) and its effect on migration and apoptosis of T47D cells. Biodiversitas 23: 572-580. Hyptis capitata Jacq. has an ethnobotanical history. The leaves and stems of H. capitata have been reported to have potential anticancer properties. This study was conducted to determine the anticancer potential of the roots, stems, leaves, and flowers of H. capitata. Organs of H. capitata were extracted in stages using chloroform and continued with methanol to obtain eight extracts. Crude extracts were tested for cytotoxicity on T47D and WiDr cells with the MTT method. Potential inhibition of cell migration by potential fraction was tested with scratch wound healing assay and the content of secondary metabolites was analyzed with gas chromatography-mass spectrometry (GC-MS). An Annexin V-Pl apoptosis test was performed to determine the number of living cells, necrosis, and apoptosis. The results showed that the root chloroform extract showed the highest cytotoxic activity against T47D cells (34.90 ± 4.78µg/mL) and WiDr (44.65 ± 12.07 µg/mL), and it was referred to as a potential extract. Fractionation of the root chloroform extract resulted in four fractions (F1, F2, F3, and F4). Fraction F2 had the highest cytotoxic activity (13.8 ± 0.65 µg/mL) and was selective against T47D cells (selectivity index 3.71). Fraction F2 showed its antimetastatic and apoptotic potential against T47D cells. Gas chromatography-mass spectrometry analyses combined with library showed that the root chloroform extract and fraction F2 contain ferruginol, campesterol, and stigmasterol.

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The potency of Sansevieria trifasciata and S. cylindrica leaves extracts as an antibacterial against Pseudomonas aeruginosa

Cited by 16 publications

References 24 publications

Acute toxicity and anticancer potential of knobweed (Hyptis capitata) ethanolic leaf extract and fraction

Acute toxicity and anticancer potential of knobweed (Hyptis capitata) ethanolic leaf extract and fraction

Docking and Molecular Dynamics Study to Identify Novel Phytobiologics from Dracaena trifasciata against Metabolic Reprogramming in Rheumatoid Arthritis

Cytotoxicity extract and fraction of knobweed (Hyptis capitata) and its effect on migration and apoptosis of T47D cells

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