Metabolic profiling by ion mobility mass spectrometry (IMMS)

Dwivedi, Prabha; Wu, Peng; Klopsch, Steve J.; Puzon, Geoffrey J.; Xun, Luying; Hill, Herbert H.

doi:10.1007/s11306-007-0093-z

Cited by 144 publications

(103 citation statements)

References 36 publications

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“…Metabolites identified in biological samples like blood plasma are often linked with disease state and require methods for identification and quantification. Metabolomics analysis by IM-MS was first investigated with direct infusion (60 ), and more recently was coupled with LC as a rapid method to analyze the human blood metabolome, allowing identification of nearly 1100 metabolites, including 300 isomers (61 ). This methodology is especially useful in identification of low-abundance compounds that can be differentiated from background noise on the basis of their mobilities.…”

Section: Ims As a Tool For Improving Metabolomics Studiesmentioning

confidence: 99%

Ion Mobility in Clinical Analysis: Current Progress and Future Perspectives

et al. 2016

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BACKGROUND Ion mobility spectrometry (IMS) is a rapid separation tool that can be coupled with several sampling/ionization methods, other separation techniques (e.g., chromatography), and various detectors (e.g., mass spectrometry). This technique has become increasingly used in the last 2 decades for applications ranging from illicit drug and chemical warfare agent detection to structural characterization of biological macromolecules such as proteins. Because of its rapid speed of analysis, IMS has recently been investigated for its potential use in clinical laboratories. CONTENT This review article first provides a brief introduction to ion mobility operating principles and instrumentation. Several current applications will then be detailed, including investigation of rapid ambient sampling from exhaled breath and other volatile compounds and mass spectrometric imaging for localization of target compounds. Additionally, current ion mobility research in relevant fields (i.e., metabolomics) will be discussed as it pertains to potential future application in clinical settings. SUMMARY This review article provides the authors' perspective on the future of ion mobility implementation in the clinical setting, with a focus on ambient sampling methods that allow IMS to be used as a “bedside” standalone technique for rapid disease screening and methods for improving the analysis of complex biological samples such as blood plasma and urine.

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Section: Ims As a Tool For Improving Metabolomics Studiesmentioning

confidence: 99%

Ion Mobility in Clinical Analysis: Current Progress and Future Perspectives

et al. 2016

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“…Together it becomes a power analytical tool for identifying molecular structure and separating complex samples which MS alone might not be able to distinguish. Studies have found the resolving power of IMMS to be in the region of which is similar to capillary gas chromatography (18 For increased accuracy, PMT-MS is usually coupled with GC to provide additional separation (20).…”

Section: Techniques For Breath Testingmentioning

confidence: 99%

Breath Analysis for Medical Diagnosis

Hill

Binions

2012

International Journal on Smart Sensing and Intelligent Systems

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The purpose of this review is to highlight the advances in technology and understanding in the field of breath analysis for medical diagnosis. A critical review of the methods of breath collection, treatment, and analysis is given, highlighting the problems facing the field and areas where promising advancement has been made. One particular area of interest is centered around electronic noses, ideally, portable devices which aim to mimic biological olifactory systems in analysing gases to produce odor fingerprints. Furthermore, recent work has shown it is possible to modify the basic sensor materials to both improve their performance, increase their tolerance to factors such as water vapour interferance which often leave the sensor system de-sensitized to the gaseous biomarkers, and enhance their selectivity. It will be shown how it is possible to accurately quantify concentrations of VOC's INTERNATIONAL JOURNAL ON SMART SENSING AND INTELLIGENT SYSTEMS, VOL. 5, NO. 2, JUNE 2012 401 and make disease diagnosis from analysis of the collected data which compare favorably with traditional medical diagnostic techniques.

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“…Among the various pulse sequence programs, 1 H-NMR has been routinely employed for high-throughput metabolomic studies due to its relatively short acquisition time per analysis. Alternatively, several conventional as well as innovative analytical instruments, such as Fourier transform infrared spectrometry [51], direct-infusion time-of-flight (TOF) MS [24,77], ion mobility MS [25], and LC-FT-ICR MS [48,91], have recently been applied to metabolomic studies.…”

Section: Analytical Technologiesmentioning

confidence: 99%

Rice Metabolomics

Oikawa

Mizutani

Kusano

et al. 2008

Rice

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Metabolomics is a recently developed technology for comprehensive analysis of metabolites in organisms. Plant metabolites that are produced for the growth, development, and chemical defense of plants against climatic alterations or natural predators are also useful to us as nutrients or medicines; hence, it is important to comprehend the amounts and varieties of plant metabolites. Besides providing an understanding of the metabolic state in plants under various circumstances, metabolomic techniques are applicable to the clarification of the functions of unknown genes by using natural variants or mutants of the target plants. Furthermore, a metabolomic approach might be useful in the breeding of crops, since valuable plant traits such as taste and yield are closely related to metabolic conditions. Here, we describe the methodology of metabolomics including sample extraction and preparation, metabolite detection, and data processing and analysis, and introduce the application of metabolomic studies to rice.

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Metabolic profiling by ion mobility mass spectrometry (IMMS)

Cited by 144 publications

References 36 publications

Ion Mobility in Clinical Analysis: Current Progress and Future Perspectives

Ion Mobility in Clinical Analysis: Current Progress and Future Perspectives

Breath Analysis for Medical Diagnosis

Rice Metabolomics

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