Metabolic Profiles Reveal Changes in Wild and Cultivated Soybean Seedling Leaves under Salt Stress

Zhang, Jing; Yang, Dong-shuang; Li, Mingxia; Shi, Lianxuan

doi:10.1371/journal.pone.0159622

Cited by 99 publications

(69 citation statements)

References 37 publications

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“…To cope with providing enough organic carbon for cellular respiration, the carbon skeletons of some amino acids might have been selectively broken down due to the induction of specific deaminase and lyase genes (Figure , Figure S4). Our results are consistent with the previously reported massive change in amino acid profiles under stress (Das et al, ; Zhang, Yang, Li, & Shi, ).…”

Section: Discussionsupporting

confidence: 94%

Transcriptomic reprogramming in soybean seedlings under salt stress

Liu

Xiao

et al. 2018

Plant Cell & Environment

108

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To obtain a comprehensive understanding of transcriptomic reprogramming under salt stress, we performed whole-transcriptome sequencing on the leaf and root of soybean seedlings subjected to salt treatment in a time-course experiment (0, 1, 2, 4, 24, and 48 hr). This time series dataset enabled us to identify important hubs and connections of gene expressions. We highlighted the analysis on phytohormone signaling pathways and their possible crosstalks. Differential expressions were also found among those genes involved in carbon and nitrogen metabolism. In general, the salt-treated seedlings slowed down their photosynthetic functions and ramped up sugar catabolism to provide extra energy for survival. Primary nitrogen assimilation was shut down whereas nitrogen resources were redistributed. Overall, the results from the transcriptomic analyses indicate that the plant uses a multipronged approach to overcome salt stress, with both fast-acting, immediate physiological responses, and longer term reactions that may involve metabolic adjustment.

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Section: Discussionsupporting

confidence: 94%

Transcriptomic reprogramming in soybean seedlings under salt stress

Liu

Xiao

et al. 2018

Plant Cell & Environment

108

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“…Organic acids, as small-molecule osmotic adjustment substances, play an important role in the physiological process of salt resistance, especially under alkali stress [59]. Our results showed that the levels of some organic acids increased significantly after AMF inoculation under alkali stress.…”

Section: Effects Of Arbuscular Mycorrhizal Fungi On the Metabolic Promentioning

confidence: 59%

Metabolomics Analysis Reveals the Alkali Tolerance Mechanism in Puccinellia tenuiflora Plants Inoculated with Arbuscular Mycorrhizal Fungi

et al. 2020

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Soil alkalization is a major environmental threat that affects plant distribution and yield in northeastern China. Puccinellia tenuiflora is an alkali-tolerant grass species that is used for salt-alkali grassland restoration. However, little is known about the molecular mechanisms by which arbuscular mycorrhizal fungi (AMF) enhance P. tenuiflora responses to alkali stress. Here, metabolite profiling in P. tenuiflora seedlings with or without arbuscular mycorrhizal fungi (AMF) under alkali stress was conducted using liquid chromatography combined with time-of-flight mass spectrometry (LC/TOF-MS). The results showed that AMF colonization increased seedling biomass under alkali stress. In addition, principal component analysis (PCA) and orthogonal projections to latent structures discriminant analysis (OPLS-DA) demonstrated that non-AM and AM seedlings showed different responses under alkali stress. A heat map analysis showed that the levels of 88 metabolites were significantly changed in non-AM seedlings, but those of only 31 metabolites were significantly changed in AM seedlings. Moreover, the levels of a total of 62 metabolites were significantly changed in P. tenuiflora seedlings after AMF inoculation. The results suggested that AMF inoculation significantly increased amino acid, organic acid, flavonoid and sterol contents to improve osmotic adjustment and maintain cell membrane stability under alkali stress. P. tenuiflora seedlings after AMF inoculation produced more plant hormones (salicylic acid and abscisic acid) than the non-AM seedlings, probably to enhance the antioxidant system and facilitate ion balance under stress conditions. In conclusion, these findings provide new insights into the metabolic mechanisms of P. tenuiflora seedlings with arbuscular mycorrhizal fungi under alkali conditions and clarify the role of AM in the molecular regulation of this species under alkali stress.

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“…In our experiment, malic acid decreased up to a approximately fourfold lower concentration compared with controls, specifically at high Cl − treatments (Figure b). The TCA cycle metabolites and malic acid as part of these substances are often changed as a result of salt stress (Widodo et al, ; Zhang, Yang, Li, & Shi, ). The new findings here are that root dehydroascorbate and leaf ascorbate change with potassium treatment but not with sodium treatment (Figure S5a–b); on the other hand, 2‐methylmalic acid, methionine, and oxomalonic acid change upon Na treatment.…”

Section: Discussionmentioning

confidence: 99%

Ion‐dependent metabolic responses of Vicia faba L. to salt stress

Richter

Behr

Erban

et al. 2018

Plant Cell & Environment

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Salt-affected farmlands are increasingly burdened by chlorides, carbonates, and sulfates of sodium, calcium, and magnesium. Intriguingly, the underlying physiological processes are studied almost always under NaCl stress. Two faba bean cultivars were subjected to low- and high-salt treatments of NaCl, Na SO , and KCl. Assimilation rate and leaf water vapor conductance were reduced to approximately 25-30% without biomass reduction after 7 days salt stress, but this did not cause severe carbon shortage. The equimolar treatments of Na , K , and Cl showed comparable accumulation patterns in leaves and roots, except for SO which did not accumulate. To gain a detailed understanding of the effects caused by the tested ion combinations, we performed nontargeted gas chromatography-mass spectrometry-based metabolite profiling. Metabolic responses to various salts were in part highly linearly correlated, but only a few metabolite responses were common to all salts and in both cultivars. At high salt concentrations, only myo-inositol, allantoin, and glycerophosphoglycerol were highly significantly increased in roots under all tested conditions. We discovered several metabolic responses that were preferentially associated with the presence of Na , K , or Cl . For example, increases of leaf proline and decreases of leaf fumaric acid and malic acid were apparently associated with Cl accumulation.

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Metabolic Profiles Reveal Changes in Wild and Cultivated Soybean Seedling Leaves under Salt Stress

Cited by 99 publications

References 37 publications

Transcriptomic reprogramming in soybean seedlings under salt stress

Transcriptomic reprogramming in soybean seedlings under salt stress

Metabolomics Analysis Reveals the Alkali Tolerance Mechanism in Puccinellia tenuiflora Plants Inoculated with Arbuscular Mycorrhizal Fungi

Ion‐dependent metabolic responses of Vicia faba L. to salt stress

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