Metabolic Products of Cholesterol in Bile and Feces of Rat. Steroids and Bile Acids

Bergström, Sune; Norman, Arne

doi:10.3181/00379727-83-20269

Cited by 66 publications

(10 citation statements)

References 1 publication

(2 reference statements)

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“…In man, however, the labeled cholic acid was present as the glycine conjugate, whereas in rat bile taurocholic acid constituted the major labeled bile salt (5,6). This finding was not surprising since glycocholic acid has long been known to be the predominant bile salt in human bile (14) and so might be expected to be an important cholesterol metabolite as well.…”

Section: Discussionmentioning

confidence: 99%

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Cholesterol Metabolism in Man

Siperstein¹,

Murray²

1955

J. Clin. Invest.

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Section: Discussionmentioning

confidence: 99%

“…In rat bile, the chief bile acid resulting from cholesterol metabolism has been identified specifically as the taurine conjugate of cholic acid (4)(5)(6).…”

mentioning

confidence: 99%

Cholesterol Metabolism in Man

Siperstein¹,

Murray²

1955

J. Clin. Invest.

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“…tin 11 mit "reversed phases 4 ' benutzt. Dabei wird durch Kieselgur, das mit Chlormethylsilan hydrophob gemacht worden ist, die weniger hydrophile Phase stationär gehalten, während durch die Säule die mehr hydrophile Phase fließt.…”

unclassified

Über den Stoffwechsel der Cholsäure und Desoxycholsäure in der Ratte. X. Mitteilung über Steroide und Gallensäuren

Bergström¹,

Rottenberg²,

Sjövall³

1953

Hoppe-Seyler´s Zeitschrift für physiologische Chemie

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“…However, 17% of the free bile acids dissolved in the light petroleum extract (H), and were recovered by fractionation of the free acids (Q). I n addition, bile-acid soaps may be present in Fractions (B) and (G), and the small proportion of conjugated bile acids found in faeces (Bergstrom & Norman, 1953) may concentrate in Fraction (L). Further investigations of these bile-acid fractions are being made in view of the comment by Bergstrom (1961) that in much previous work analysis for total bile acids has apparently accounted for only part of this fraction.…”

Section: Rile Acidsmentioning

confidence: 99%

Lipid excretion

Aylward¹,

Wood²

1962

Br J Nutr

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The routine clinical laboratory methods used in metabolic studies on lipids normally provide information about a very limited number of fractions, such as free and bound fatty acids, total unsaponifiable matter and sterols precipitable by digitonin or giving acolour reaction with the Liebermann-Burchard or other reagent. The newer chromatographic and allied techniques applied to blood and various tissues have revealed the complexity of lipid mixtures and the inadequacies of the classical methods which have necessarily formed the basis of most clinical investigations, including many on the relationship of diet to composition of plasma lipids.I n recent years considerable progress has been made in the separation and characterization of lipids of plasma and other tissues by, for example, chromatography on silicic acid columns and gas-liquid chromatography, and these methods have been used in many recent studies (e.g. Bottcher, Woodford, Boelsma-van Houte & van Gent, 1959). However, comparatively little work has been done on faecal lipids by these newer techniques and in particular there is a lack of detailed information on steroid fractions.I n our own investigations we have used colorimetric and digitonin precipitation methods for sterols (Aylward & Wills, 1962) recognizing that the information given is limited because of the difficulty in determining specific sterols (such as cholesterol) in mixtures, but we have shown also (Aylward & Wills, 1961) that chromatographic methods can be applied to faecal lipids and that it is possible to identify, for example, sterol esters.It became clear during these studies and from a survey of older and recent papers that faecal lipid mixtures were in many respects more complex than those from any other source and that it would be difficult to devise improved routine methods for nutritional and general metabolic studies until the different components of faecal lipids were more thoroughly examined. It has become evident also that the importance of balance studies on human subjects is now accepted by an increasing number of workers (e.g. Aylward, 1958; Hellman & Rosenfeld, 1959 This paper describes the fractionation of faecal lipids and related compounds by chromatographic and other techniques. T h e application of these methods in metabolism experiments will be considered in subsequent publications. EXPERIMENTAL AND RESULTS Extraction of faeces and preliminary fractionationSamples of faeces were obtained from normal subjects and treated in the way shown in Fig. I.Extraction of lipid. I n our experiments special attention was given to the initial extraction techniques; methods involving the air drying of faeces had to be avoided because of oxidation not only of fatty acids but of other unsaturated compounds, including sterols. Methods involving the preliminary saponification of lipids were also ruled out because they result in the breakdown of esters so that the fatty acids in the different fractions cannot be identified. Fractions were stored at -3 5 O and operations were carried ...

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Metabolic Products of Cholesterol in Bile and Feces of Rat. Steroids and Bile Acids

Cited by 66 publications

References 1 publication

Cholesterol Metabolism in Man

Cholesterol Metabolism in Man

Über den Stoffwechsel der Cholsäure und Desoxycholsäure in der Ratte. X. Mitteilung über Steroide und Gallensäuren

Lipid excretion

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