Metabolic modeling of fumaric acid production byrhizopus arrhizus

Gangl, Irene C.; Weigand, William A.; Keller, Frederick A.

doi:10.1007/bf02922626

Cited by 6 publications

(5 citation statements)

References 11 publications

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“…In conclusion, despite the reductive reactions of the TCA cycle, which coexist with the oxidative ones, are generally regarded as playing a predominant role in fumarate accumulation,7, 17 the main results of this study appear to reverse such a general belief. However, more work is still needed to assess further the respective contributions of reductive and oxidative reactions of TCA cycle in fumarate production.…”

Section: Resultscontrasting

confidence: 60%

“…Fumarate is synthesised mainly from pyruvate via a carboxylation reaction yielding oxaloacetate, being subsequently converted into malate and further into fumarate via the reductive (or reverse) reactions of the tricarboxylic acid (TCA) cycle 5. 7, 17 For ATP generation the oxidative (or forward) reactions of TCA cycle result in the formation of fumarate from citrate and succinate. Of the intermediate compounds, malic acid is predominant,17 conversion into fumaric acid being controlled, under the reductive reaction conditions, by cytosolic activity of fumarase 18…”

Section: Resultsmentioning

confidence: 99%

“…Laboratory‐ and pilot‐plant production of fumaric acid by submerged‐culture fermentations was first reported by the research laboratories of the US Department of Agriculture4, 5 and Rhizopus arrhizus NRRL 1526 has been used to optimise the fermentation process 6–11…”

Section: Introductionmentioning

confidence: 99%

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Ammonium fumarate production by free or immobilised Rhizopus arrhizus in bench‐ and laboratory‐scale bioreactors

Riscaldati

Moresi

Federici

et al. 2002

J of Chemical Tech & Biotech

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Ammonium fumarate production from glucose-based media by Rhizopus arrhizus NRRL 1526 with mycelial growth controlled by phosphorus limitation exhibited mixed-growth-associated product formation kinetics, with growth-associated production related to secondary mycelial growth only. The contribution of the primary mycelial growth phase was minimised by resorting to prolonged batch production using free mycelia under intermittent glucose feeding or repeated batch production using immobilised mycelia. The metabolic activity of free or immobilised mycelia was limited by fumarate accumulation or by oxygen diffusion phenomena, respectively. For batch cultures in a 15 dm 3 stirred bioreactor the peripheral impeller speed (v I ) was increased from 1.88 to 3.3 m s À1 , and the fumarate yield coef®cient on glucose increased from 0.25 AE 0.01 to 0.42 AE 0.02 g g À1

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Section: Resultscontrasting

confidence: 60%

Section: Resultsmentioning

confidence: 99%

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Ammonium fumarate production by free or immobilised Rhizopus arrhizus in bench‐ and laboratory‐scale bioreactors

Riscaldati

Moresi

Federici

et al. 2002

J of Chemical Tech & Biotech

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“…The problem arose in part from the inactivation of key enzymes such as pyruvate carboxylase and fumarase involved in the fumaric acid formation pathway [5,16]. On the other hand, in terms of the mutant numbered ME-UN-1, the production of lactic acid was enhanced during fumaric acid production; this might have been caused by the activation of lactate dehydrogenase (LDH) which catalyzed pyruvate to lactic acid [18,19]. However, the rest of the three mutants numbered ME-UN-3, 4 and 8 showed a higher fumaric acid production and high stability with little regression after ten generations (data not shown), while having no significant change in malic and lactic acid production with the ethanol production decreased sharply ( Table 2).…”

Section: Analysis Of Various Metabolites Formed By Different Mutantsmentioning

confidence: 99%

Strain improvement of Rhizopus oryzae for over-production of fumaric acid by reducing ethanol synthesis pathway

et al. 2010

Korean J. Chem. Eng.

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Rhizopus oryzae mutants were isolated from the wild type strain ME-F01 after mutagenesis with UV coupled with nitrosoguanidine (NTG) and the following allyl alcohol resistance selection method. By analyzing the activities of alcohol dehydrogenase (ADH) involved in ethanol synthesis pathway and batch fermentation, one mutant, ME-UN-8, was isolated that produced 21.1% more fumaric acid than ME-F01 with the corresponding byproduct of ethanol decreased by 83.7%.

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“…By limiting the nitrogen source, Rhizopus growth can be minimized. During this nongrowth stage, fumaric acid can be accumulated with a maximal yield of 2 mol/mol of glucose consumed or 1.29 g of fumarate per g of glucose consumed on a weight basis (4,5). In reality, however, the reductive bypass requires supply of NADH from the tricarboxylic acid cycle.…”

mentioning

confidence: 99%

Simultaneous Production and Recovery of Fumaric Acid from Immobilized Rhizopus oryzae with a Rotary Biofilm Contactor and an Adsorption Column

Cao¹,

Du²,

Gong³

et al. 1996

Appl Environ Microbiol

129

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An integrated system of simultaneous fermentation-adsorption for the production and recovery of fumaric acid from glucose by Rhizopus oryzae was investigated. The system was constructed such that growing Rhizopus mycelia were self-immobilized on the plastic discs of a rotary biofilm contactor during the nitrogen-rich growth phase. During the nongrowth, production phase, the biofilm was alternately exposed to liquid medium and air upon rotation of the discs in the horizontal fermentation vessel. The product of fermentation, fumaric acid, was removed simultaneously and continuously by a coupled adsorption column, thereby moderating inhibition, enhancing the fermentation rate, and sustaining cell viability. Another beneficial effect of the removal of fumaric acid is release of hydroxyl ions from a polyvinyl pyridine adsorbent into the circulating fermentation broth. This moderates the decrease in pH that would otherwise occur. Polyvinyl pyridine and IRA-900 gave the highest loading for this type of fermentation. This fermentation system is capable of producing fumaric acid with an average yield of 85 g/liter from 100 g of glucose per liter within 20 h under repetitive fed-batch cycles. On a weight yield basis, 91% of the theoretical maximum was obtained with a productivity of 4.25 g/liter/h. This is in contrast to stirred-tank fermentation supplemented with calcium carbonate, whose average weight yield was 65% after 72 h with a productivity of 0.9 g/liter/h. The immobilized reactor was operated repetitively for 2 weeks without loss of biological activity.

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Metabolic modeling of fumaric acid production byrhizopus arrhizus

Cited by 6 publications

References 11 publications

Ammonium fumarate production by free or immobilised Rhizopus arrhizus in bench‐ and laboratory‐scale bioreactors

Ammonium fumarate production by free or immobilised Rhizopus arrhizus in bench‐ and laboratory‐scale bioreactors

Strain improvement of Rhizopus oryzae for over-production of fumaric acid by reducing ethanol synthesis pathway

Simultaneous Production and Recovery of Fumaric Acid from Immobilized Rhizopus oryzae with a Rotary Biofilm Contactor and an Adsorption Column

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