Metabolic analysis of two contrasting wild barley genotypes grown hydroponically reveals adaptive strategies in response to low nitrogen stress

Quan, Xiaoyan; Qian, Qiufeng; Ye, Zhilan; Zeng, Jianbin; Zhao, Han; Zhang, Guoping

doi:10.1016/j.jplph.2016.07.020

Cited by 39 publications

(32 citation statements)

References 48 publications

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“…Our previous study showed that XZ141 had the lowest K content in shoots under LK among the three barley genotypes [ 16 ]. Moreover, it was also reported that sucrose transported from leaf to root acts as a signal molecule involved in root growth regulation in response to nutrition deficiency [ 25 , 47 ]. Coincidentally, we found that only in XZ141 RFW was significantly reduced under LK stress compared to its control (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Actually, many metabolomics studies have been conducted to understand the mechanisms of abiotic stress tolerance in plants, including drought [ 20 ], salinity [ 21 ], combined stress of drought and high temperature [ 22 ] and cadmium toxicity [ 23 ]. Meanwhile, metabolomics analysis have also been used in revealing the mechanisms of low nutrient tolerance in higher plants, such as the responses of maize and barley to low nitrogen stress [ 24 , 25 ], of common beans and barley to P deficiency [ 26 , 27 ] and of Arabidopsis to low sulfur level [ 28 ]. Moreover, metabolite profiles were also reported in Arabidopsis and tomato plants exposed to normal and low-K conditions [ 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

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Root and leaf metabolite profiles analysis reveals the adaptive strategies to low potassium stress in barley

Zeng

Quan

et al. 2018

BMC Plant Biol

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BackgroundPotassium (K) deficiency in arable land is one of the most important factors affecting crop productivity. Development of low K (LK) tolerant crop cultivars is regarded as a best economic and effective approach for solving the issue of LK. In previous studies, we found a wider variation of LK tolerance in the Tibetan wild barley accessions than cultivated barley. However, the mechanism of LK tolerance in wild barley is still elusive.ResultsIn this study, two wild barley genotypes (XZ153, LK tolerant and XZ141, LK sensitive) and one cultivar (LuDaoMai, LK tolerant) was used to investigate metabolome changes in response to LK stress. Totally 57 kinds of metabolites were identified in roots and leaves of three genotypes at 16 d after LK treatment. In general, accumulation of amino acids and sugars was enhanced in both roots and leaves, while organic acids were reduced under LK stress compared to the control. Meanwhile, the concentrations of the negatively charged amino acids (Asp and Glu) and most organic acids was reduced in both roots and leaves, but more positively charged amino acids (Lys and Gln) were increased in three genotypes under LK. XZ153 had less reduction than other two genotypes in biomass and chlorophyll content under LK stress and showed greater antioxidant capacity as reflected by more synthesis of active oxygen scavengers. Higher LK tolerance of XZ153 may also be attributed to its less carbohydrate consumption and more storage of glucose and other sugars, thus providing more energy for plant growth under LK stress. Moreover, phenylpropanoid metabolic pathway mediated by PAL differed among three genotypes, which is closely associated with the genotypic difference in LK tolerance.ConclusionsLK tolerance in the wild barley is attributed to more active phenylpropanoid metabolic pathway mediated by PAL, energy use economy by reducing carbohydrate consumption and storage of glucose and other sugars, and higher antioxidant defense ability under LK stress.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1404-4) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Root and leaf metabolite profiles analysis reveals the adaptive strategies to low potassium stress in barley

Zeng

Quan

et al. 2018

BMC Plant Biol

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“…Plant metabolomics research is important in understanding plant metabolic pathways, improving plant response to unfavorable environmental stresses and ameliorating crop yield and quality (Saito and Matsuda , Adamski and Suhre ). Metabolomics studies have been applied to the response of different species to LN stress, including tomato, tall fescue and wild barley (Urbanczyk‐Wochniak and Fernie , Li et al , Quan et al ). In these cases, metabolomic analyses provide deep and valuable insight into the molecular responses to LN stress.…”

Section: Introductionmentioning

confidence: 99%

Physiological characteristics and metabolomics reveal the tolerance mechanism to low nitrogen in Glycine soja leaves

Zhao

Guo

et al. 2019

Physiologia Plantarum

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To explore the regulatory mechanisms involved in the adaption to nitrogen (N) deficiency of wild soybean, the ion balance, photosynthetic characteristics, metabolic and transcriptional changes in leaves of common and low N (LN)-tolerant wild soybean seedlings under LN stress were determined. The LN-tolerant wild soybean seedlings showed a stronger ability to maintain photosynthesis and nutrient balance than common wild soybean. A total of 52 differentially accumulated metabolites, mainly related to carbon and N metabolism, were identified between the control and the LN treatment group. In general, tricarboxylic acid (TCA) cycle, shikimic acid pathway, synthetase/glutamate synthase (GS/GOGAT) cycle and accumulation of most organic acids were enhanced in LN-tolerant wild soybean, while reduced in common wild soybean under LN stress compared with their respective control group. Moreover, glycolysis, sugar and polyol and fatty acid metabolism increased in both wild soybean genotypes, and increased more in LN-tolerant wild soybean. A total of 3381 differentially expressed genes (DEGs) were identified in leaves of both wild soybean genotypes and the expressed level of DEGs associated with sugars, polyols, fatty acids and energy metabolism was significantly higher in LN-tolerant wild soybean than in common wild soybean, consistent with changes in metabolite level. Our results suggest new ideas for the study of LN tolerance of wild soybean and provide a theoretical basis for development and utilization of wild soybean resources.

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“…As China is the largest producer and consumer of N fertilizers, and the management of N pollution including N-related greenhouse gas emissions was more urgent and essential to global efforts ( Zhang et al, 2013 ). Improved crop varieties with high N-use efficiency (NUE) are considered the best economic strategy for agricultural sustainability ( Quan et al, 2016 ). Previous studies have shown that genetic improvement of NUE in crops is feasible ( Ding et al, 2005 ; Namai et al, 2009 ; Quan et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

“…Improved crop varieties with high N-use efficiency (NUE) are considered the best economic strategy for agricultural sustainability ( Quan et al, 2016 ). Previous studies have shown that genetic improvement of NUE in crops is feasible ( Ding et al, 2005 ; Namai et al, 2009 ; Quan et al, 2016 ). However, the narrow genetic base of barley has limited genetic improvement of NUE.…”

Section: Introductionmentioning

confidence: 99%

Rapid Generation of Barley Mutant Lines With High Nitrogen Uptake Efficiency by Microspore Mutagenesis and Field Screening

et al. 2018

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In vitro mutagenesis via isolated microspore culture provides an efficient way to produce numerous double haploid (DH) lines with mutation introduction and homozygosity stabilization, which can be used for screening directly. In this study, 356 DH lines were produced from the malt barley (Hordeum vulgare L.) cultivar Hua-30 via microspore mutagenic treatment with ethyl methane sulfonate or pingyangmycin during in vitro culture. The lines were subjected to field screening under high nitrogen (HN) and low nitrogen (LN) conditions, and the number of productive tillers was used as the main screening index. Five mutant lines (A1-28, A1-84, A1-226, A16-11, and A9-29) with high numbers of productive tillers were obtained over three consecutive years of screening. In the fifth year, components related to N-use efficiency (NUE), including N accumulation, utilization, and translocation, were characterized for these lines based on N uptake efficiency (NUpE), N utilization efficiency (NUtE), and N translocation efficiency (NTE). The results show that the NUpE of four mutant lines (A1-84, A1-226, A9-29, and A16-11) improved significantly under HN, whereas that of two lines (A1-84 and A9-29) improved under LN. As a result, their NUE improved greatly. No improvement in NUtE was observed in any of the five mutant lines. A1-84 and A9-29 were selected as an enhanced genotype in N uptake, and A1-28 showed improved NTE at the grain-filling stage. Our results imply that high-NUpE mutants can be produced through microspore mutagenesis and field screening, and that improvement of NUE in barley depends on enhancement of N uptake.

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Metabolic analysis of two contrasting wild barley genotypes grown hydroponically reveals adaptive strategies in response to low nitrogen stress

Cited by 39 publications

References 48 publications

Root and leaf metabolite profiles analysis reveals the adaptive strategies to low potassium stress in barley

Root and leaf metabolite profiles analysis reveals the adaptive strategies to low potassium stress in barley

Physiological characteristics and metabolomics reveal the tolerance mechanism to low nitrogen in Glycine soja leaves

Rapid Generation of Barley Mutant Lines With High Nitrogen Uptake Efficiency by Microspore Mutagenesis and Field Screening

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