Mesothelial cell transplantation in the infarct scar induces neovascularization and improves heart function

Elmadbouh, Ibrahim; Chen, Ying; Louedec, Liliane; Pouzet, Bruno; Meilhac, Olivier; Michel, Jean‐Baptiste

doi:10.1016/j.cardiores.2005.05.022

Cited by 37 publications

(24 citation statements)

References 38 publications

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“…SDF-1α protein and gene delivery to the ischemic heart promotes endothelial progenitor cells recruitment into the ischemic myocardium to promote angiogenesis [1,2,3]. SDF-1α specifically interacts with the CXCR4 receptor and orchestrates the mobilization and homing of hematopoietic stem cells from bone marrow (BM) to the ischemic heart [3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

“…In order to overcome this deficiency, SDF-1α concentration gradient in favor of myocardial scar has been achieved by the delivery of SDF-1α protein or gene encoding for hSDF-1α. The delivery strategies include intramyocardial injection of SDF-1α protein [2], naked plasmid encoding for SDF-1α [3,5,6], transplantation of ex vivo genetically manipulated cardiac fibroblasts overexpressing SDF-1α [4] and mesothelial cell transplantation which intrinsically express copious amounts of SDF-1α [1,14]. Moreover, intramuscular injections of GCSF (Filgrastim) 4 , the sulfated polysaccharide (Fucoidan) [15][16] or "3 hydroxy-3-methylglutaryl-CoA reductase inhibitor" (Atorvastatin) [17][18] have been reported to increase the plasma concentration of hSDF-1α to a level sufficient enough to induce mobilization of stem cells from BM and their homing into the injured tissue.…”

Section: Introductionmentioning

confidence: 99%

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Ex vivo delivered stromal cell-derived factor-1α promotes stem cell homing and induces angiomyogenesis in the infarcted myocardium

Elmadbouh

Haider

Jiang

et al. 2007

Journal of Molecular and Cellular Cardiology

167

110

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We aimed to optimize non-viral transfection of human stromal cell derived factor (SDF-1α) gene into skeletal myoblasts (SkM) and, transplant these cells to establish transient SDF-1α gradient to favor extra-cardiac stem cell translocation into infarcted heart. Optimized conditions for transfection of SDF-1α gene into syngenic SkM were achieved using FuGene™6/phSDF-1α (3:2v/w, 4h transfection) with 125μM ZnCl 2 (p<0.001). After characterization for transgene overexpression by immunostaining, ELISA, and PCR, the cells were transplanted in female rat model of myocardial infarction. Thirty-six rats were grouped (n=12/group) to receive 70μl DMEM without cells (group-1) or containing 1.5×10 6 non-transfected (group-2) or SDF-1α transfected SkM (group-3). On day-4 post-transplantation (in 4 animals/group), marked expression of SDF-1α/sry-gene (p=0.003), total Akt, phospho-Akt and Bcl2 was observed in group-3. The number of CD31 + , C-kit + and CD34 + cells was highest in group-3 hearts (p<0.01). Blood vessel density in group-3 was higher in both scar and peri-scar regions (p<0.001) as compared with other groups. Echocardiography showed improved indices of left ventricle contractile function and remodeling in group-3 (p<0.05) as compared with groups-1 and -2. We conclude that ex-vivo SDF-1α transgene delivery promotes stem and progenitor cell migration to the heart, activates cell survival signaling and enhances angiomyogenesis in the infarcted heart.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ex vivo delivered stromal cell-derived factor-1α promotes stem cell homing and induces angiomyogenesis in the infarcted myocardium

Elmadbouh

Haider

Jiang

et al. 2007

Journal of Molecular and Cellular Cardiology

167

110

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“…They distribute widely on the surface of omentum and peritoneum and are easy to harvest with a small umbilical incision. They are also easy to be cultured in vitro and the commonly used DMEM medium could satisfy their nutritional requirement [10,11]. Mesothelium-lined grafts including peritoneum [12,13] and tunica vaginalis [14,15] have already been attempted in urethral reconstruction with good results.…”

Section: Discussionmentioning

confidence: 99%

Tubularized Urethral Replacement Using Tissue-Engineered Peritoneum-Like Tissue in a Rabbit Model

Xia²,

Zhang³

et al. 2012

Urol Int

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Introduction: This study aims to investigate whether mesothelial cells could function as seed cells to construct tissue-engineered peritoneum-like tissue for urethral reconstruction in a rabbit model. Materials and Methods: Bladder acellular matrices were prepared and trimmed to 1.5 × 1 cm. Nine male rabbits underwent omentum biopsy and autologous mesothelial cells were isolated. After in vitro expansion, the cells were seeded onto the matrices and incubated for 7 days. In 18 rabbits, a pendulous urethral segment 1.5 cm long was totally excised and urethroplasty was performed with tubularized matrices seeded with cells in 9 animals and without cells in 9 as controls. Urethrography was performed at 1, 2 and 6 months postoperatively. Meanwhile, the neourethras were harvested and analyzed grossly and histologically. Results: Histological analysis of the cell-seeded grafts revealed a loose collagen matrix covered with a single layer of mesothelim. Serial urethrography indicated a wide urethral caliber without stricture formation in animals implanted with cell-seeded matrices, while all animals of the control group developed stricture. Histological analysis of the implanted cell-seeded matrices demonstrated a normal urethral architecture by 1 month, composed of multilayers of urothelium surrounded by smooth muscle bundles, which became increasingly organized with time. By 6 months, the neourethra could be hardly distinguished from native urethra both grossly and histologically. Conclusions: Autologous mesothelial cells could be successfully used as seed cells for tubularized urethral reconstruction in male rabbits.

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“…When transplanted into the damaged myocardium, MCs induced neoangiogenesis in the infarct scar and preserved heart function [23].…”

Section: Cells Employed For Experimental Car-diac Regenerationmentioning

confidence: 99%

New Perspectives to Repair a Broken Heart

Gaetani¹,

Barile

Forte

et al. 2009

CHAMC

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The aim of cardiac cell therapy is to restore at least in part the functionality of the diseased or injured myocardium by the use of stem/progenitor cells. Recent clinical trials have shown the safety of cardiac cell therapy and encouraging efficacy results. A surprisingly wide range of non-myogenic cell types improves ventricular function, suggesting that benefits may result in part from mechanisms that are distinct from true myocardial regeneration. While clinical trials explore cells derived from skeletal muscle and bone marrow, basic researchers are investigating sources of new cardiomyogenic cells, such as resident myocardial progenitors and embryonic stem cells. In this commentary we briefly review the evolution of cell-based cardiac repair, some progress that has been made toward this goal, and future perspectives in the regeneration of cardiac tissue.

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Mesothelial cell transplantation in the infarct scar induces neovascularization and improves heart function

Abstract: Since autologous MCs can be obtained easily and cultured in large quantities, MC transplantation may represent a new angiogenic strategy in the prevention of ischemic remodeling.

Cited by 37 publications

References 38 publications

Ex vivo delivered stromal cell-derived factor-1α promotes stem cell homing and induces angiomyogenesis in the infarcted myocardium

Ex vivo delivered stromal cell-derived factor-1α promotes stem cell homing and induces angiomyogenesis in the infarcted myocardium

Tubularized Urethral Replacement Using Tissue-Engineered Peritoneum-Like Tissue in a Rabbit Model

New Perspectives to Repair a Broken Heart

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