Mesenchymal Stem Cells in Combination with Hyaluronic Acid for Articular Cartilage Defects

Abstract

Mesenchymal stem cells (MSCs) and hyaluronic acid (HA) have been found in previous studies to have great potential for medical use. This study aimed to investigate the therapeutic effects of bone marrow mesenchymal stem cells (BMSCs) combined with HA on articular cartilage repair in canines. Twenty-four healthy canines (48 knee-joints), male or female with weight ranging from 5 to 6 kg, were operated on to induce cartilage defect model and divided into 3 groups randomly which received different treatments: BMSCs plus HA (BMSCs-HA), HA alone, and saline. Twenty-eight weeks after treatment, all canines were sacrificed and analyzed by gross appearance, magnetic resonance imaging (MRI), hematoxylin-eosin (HE) staining, Masson staining, toluidine blue staining, type II collagen immunohistochemistry, gross grading scale and histological scores. MSCs plus HA regenerated more cartilage-like tissue than did HA alone or saline. According to the macroscopic evaluation and histological assessment score, treatment with MSCs plus HA also lead to significant improvement in cartilage defects compared to those in the other 2 treatment groups (P < 0.05). These findings suggested that allogeneic BMSCs plus HA rather than HA alone was effective in promoting the formation of cartilage-like tissue for repairing cartilage defect in canines.

PLGA/COL I Composite Scaffold Printed by a Low-temperature Deposition Manufacturing Technique for Application to Cartilage Tissue Engineering

“…Glycosaminoglycan is an alkaline glycol polymer that becomes heterochromatic when it is reacted with the alkaline dye toluidine blue [13]. In our study, blue-purple heterochromatic granules were observed in chondrocytes, and small amounts of heterochromatic granules were observed around the chondrocytes, indicating that glycosaminoglycan and other extracellular matrix components were secreted by the chondrocytes.…”

Mesenchymal stem cell basic research and applications in dog medicine

“…This is mainly aimed to prevent health issues of the pets. UCB may not be suitable source to harvest MSCs due to limited available volume of blood and cell concentration therein (Lim et al, BM-MSC CD44, CD73, CD90, CD105 CD14, CD34, CD45 Neurogenic Ryu et al (2012) BM-MSC CD29, CD44, CD90, OCT3/4, SOX2, NANOG CD34, CD45, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81 (Continues) replacements (Enciso et al, 2018;Li et al, 2018). After confluent growth of 70-80%, the cells are enzymatically detached to reseed to lower densities and passaged for continual growth and expansion (Gade et al, 2013;Gugjoo et al, 2015).…”

Fine‐Tunable and Injectable 3D Hydrogel for On‐Demand Stem Cell Niche

“…[12] Although there are various types of biocompatible and injectable stem cell scaffolds such as poly(lactic acid) derivate, [13] hyaluronic acid, [14] and supramolecular hydrogel, [15] the controlled differentiation of stem cells is of foremost importance to the attainment of the desired tissue construction. [12] Although there are various types of biocompatible and injectable stem cell scaffolds such as poly(lactic acid) derivate, [13] hyaluronic acid, [14] and supramolecular hydrogel, [15] the controlled differentiation of stem cells is of foremost importance to the attainment of the desired tissue construction.…”