Menaquinone Composition of Some Sporeforming Actinomycetes

Collins, Matthew D.; Faulkner, Mark; Keddie, R. M.

doi:10.1016/s0723-2020(84)80048-x

Cited by 63 publications

(34 citation statements)

References 21 publications

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“…Methyl esters of cellular fatty acids were analysed by TechnoSuruga Co., Ltd according to the instructions of the Microbial Identification System (MIDI) by using a gas chromatograph (model HP6890; Hewlett Packard) (Sasser, 1990). Isoprenoid quinones were extracted according to the method of Collins et al (1977Collins et al ( , 1984 and were analysed in an HPLC system (model 802-SC; Jasco) equipped with a CAPCELL PAK C18 column (Shiseido) (Tamaoka et al, 1983). Preparation and detection of methyl esters of mycolic acids were carried out as described by Tomiyasu (1982).…”

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confidence: 99%

Actinomadura bangladeshensis sp. nov. and Actinomadura chokoriensis sp. nov.

Ara

Matsumoto

Bakir

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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The taxonomic position of two soil isolates, 3-46-b(3) T and 3-45-a(11) T , was clarified based on data from a polyphasic study. The organisms showed a combination of chemotaxonomic and morphological properties typical of the genus Actinomadura. They formed distinct phyletic lines in the Actinomadura 16S rRNA gene tree and were closely associated with the type strains of Actinomadura meyerae (sequence similarity of 98.3-98.5 %), Actinomadura napierensis (98.1-98.3 %) and Actinomadura latina (96.4-96.8 %). The level of 16S rRNA gene sequence similarity between the new isolates was 99.1 %. The level of DNA-DNA hybridization between strains 3-46-b(3) T and 3-45-a(11) T was 43.6 % and levels of relatedness between the two new isolates and the type strains of A. meyerae and A. napierensis were 21.0-27.3 %. On the basis of phenotypic and genotypic properties, the new isolates could be differentiated from each other and from their closest phylogenetic relatives. It is proposed that the organisms be classified as representing two novel species of the genus Actinomadura. The names proposed for these taxa are Actinomadura bangladeshensis sp. nov. [type strain 3-46-b(3) T 5JCM 13933 T 5MTCC8057 T ] and Actinomadura chokoriensis sp. nov. [type strain 3-45-a(11) T 5JCM 13932 T 5MTCC8056 T ].The application of chemosystematic, numerical phenetic and molecular systematic methods has clarified the taxonomy of the genus Actinomadura and related taxa (Poscher et al., 1985;Kroppenstedt et al., 1990;Ochi et al., 1991;Trujillo & Goodfellow, 1997Quintana et al., 2003a;Lee & Jeong, 2006;Le Roes & Meyers, 2007 (Henssen, 1957;Zhang et al., 2001). Members of these genera form distinct phyletic lines in trees based on 16S rRNA gene sequences (Zhang et al., 1998(Zhang et al., , 2001) and can be distinguished from one another by using a combination of biochemical, chemical, morphological and physiological features (Zhang et al., 1998(Zhang et al., , 2001Trujillo & Goodfellow, 2003). At the time of writing, the genus Actinomadura encompasses 37 recognized species, which can be delineated based on a combination of genotypic and phenotypic data (Kroppenstedt & Goodfellow, 1991;Zhang et al., 2001;Lu et al., 2003;Lee & Jeong, 2006;Le Roes & Meyers, 2007).As part of a screening programme for antibiotic-producing actinomycetes, strains 3-46-b(3) T and 3-45-a(11) T were isolated from sandy soil collected at Chokoria, Cox's Bazar, Bangladesh. The two strains showed morphological characteristics typical of the genera Actinomadura and Nonomuraea. Here we describe the characterization and classification of these strains and propose that they represent two novel species of the genus Actinomadura.Bacterial strains were isolated by using the standard dilution plate method and were grown on humic acid-vitamin agar (Hayakawa & Nonomura, 1987) supplemented with cycloheximide (50 mg l 21 ), nystatin (50 mg l 21 ) and A neighbour-joining tree based on nearly-complete 16S rRNA gene sequences showing the relationships between strains 3-46-b(3) T , 3-45-a(11) T a...

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confidence: 99%

Actinomadura bangladeshensis sp. nov. and Actinomadura chokoriensis sp. nov.

Ara

Matsumoto

Bakir

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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“…*Data for whole cell sugars, menaquinones and polar lipids were taken from: a, Tomita et al [37]; b, Horan and Brodsky [45]; c, Collins et al [46]; d, Kirby and Meyers [6]; e, Hirsch et al [39].…”

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confidence: 99%

Micromonospora terminaliae sp. nov., an endophytic actinobacterium isolated from the surface-sterilized stem of the medicinal plant Terminalia mucronata

Kaewkla

Thamchaipenet

Franco

2017

International Journal of Systematic and Evolutionary Microbiology

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An endophytic actinobacterium, strain TMS7 T , was isolated from the stem of a Thai medicinal plant collected from the grounds of the Phujong-Nayoa National park, Ubon Ratchathani province, Thailand. As a result of a polyphasic taxonomy study, this strain was identified as a member of the genus Micromonospora. This strain was a Gram-stain-positive, aerobic actinobacterium with well-developed substrate mycelium with hyphae forming a single microspore was non-motile. Stran TMS7 T was identified according to its 16S rRNA gene sequence as a new member of the genus Micromonospora. The closest phylogenetic members sharing a similarity were Micromonospora chersina DSM 44151 T at 99.4 % and Micromonospora rosaria DSM 803 T , Micromonospora tulbaghiae TVU1 T , Micromonospora inositola DSM 43819 T and Micromonospora endolithica DSM 44398 T all at 99.2 %. Chemotaxonomic data including cell wall components, major menaquinones and major fatty acids confirmed the affiliation of strain TMS7 T to the genus Micromonospora. The results of the phylogenetic analysis, addition to physiological and biochemical studies in combination with DNA-DNA hybridization, allowed the genotypic and phenotypic differentiation of strain TMS7 T and the most closely related species with validly published names. The name proposed for the novel species is Micromonospora terminaliae sp. nov. The type strain is TMS7 T (=DSM 101760 T =NRRL B-65345 T ).

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“…Methyl esters of cellular fatty acids were prepared and analysed according to the instructions of the Microbial Identification System (Sherlock Microbial Identification System; Hewlett Packard) (Sasser, 1990). Isoprenoid quinones were extracted using the method of Collins et al (1977Collins et al ( , 1984 and were analysed by HPLC with a Cosmosil 5C 18 column (4.66150 mm; Nacalai Tesque) (Tamaoka et al, 1983) and mass spectrometry (GCMS QP5050; Shimadzu). The preparation and detection of methyl esters of mycolic acids were carried out as described by Tomiyasu (1982).…”

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confidence: 99%