Membrane chromatography reactor system for the continuous synthesis of galactosyl-oligosaccharides

Engel, L. David; Ebrahimi, Mehrdad; Czermak, Peter

doi:10.1016/j.desal.2007.04.078

Cited by 21 publications

(11 citation statements)

References 13 publications

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“…Engel et al [28], studying the GOS synthesis by β-galactosidase from Kluyveromyces lactis, and Chen et al [29], studying three β-galactosidases from Aspergillus oryzae, K. lactis, and Bacillus sp. in the GOS production, verified that the galactose concentration was smaller than the glucose concentration, showing the galactose consumption by the microorganisms for GOS production.…”

Section: Resultsmentioning

confidence: 98%

Maximization of β-Galactosidase Production: A Simultaneous Investigation of Agitation and Aeration Effects

Alves

Filho

Burkert

et al. 2009

Appl Biochem Biotechnol

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In this work, the agitation and aeration effects in the maximization of the beta-galactosidase production from Kluyveromyces marxianus CCT 7082 were investigated simultaneously, in relation to the volumetric enzyme activity and the productivity, as well as the analysis of the lactose consumption and production of glucose, and galactose of this process. Agitation and aeration effects were studied in a 2 L batch stirred reactor. A central composite design (2(2) trials plus three central points) was carried out. Agitation speed varied from 200 to 500 rpm and aeration rate from 0.5 to 1.5 vvm. It has been shown in this study that the volumetric enzyme production was strongly influenced by mixing conditions, while aeration was shown to be less significant. Linear models for activity and productivity due to agitation and aeration were obtained. The favorable condition was 500 rpm and 1.5 vvm, which lead to the best production of 17 U mL(-1) for enzymatic activity, 1.2 U mL(-1) h(-1) for productivity in 14 h of process, a cellular concentration of 11 mg mL(-1), and a 167.2 h(-1) volumetric oxygen transfer coefficient.

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Section: Resultsmentioning

confidence: 98%

Maximization of β-Galactosidase Production: A Simultaneous Investigation of Agitation and Aeration Effects

Alves

Filho

Burkert

et al. 2009

Appl Biochem Biotechnol

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“…The chemical synthesis of GOS requires multiple protection and de-protection steps of specific molecular groups, possessing a complexity causing chemically driven synthesis little attractive for industrial applications. To overcome this challenge, new strategies for the continuous production of GOS in innovative enzymatic membrane bioreactors have been previously developed [2][3][4][5][6][7][8]. Hence, enzymatic synthesis of oligosaccharides and their derivatives have been shown as promising alternatives to chemical synthesis.…”

Section: Introductionmentioning

confidence: 99%

A novel ceramic membrane reactor system for the continuous enzymatic synthesis of oligosaccharides

Ebrahimi¹,

Placido²,

Engel³

et al. 2010

Desalination

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The continuous enzymatic production of galactosyl-oligosaccharides (GOS) from lactose as a substrate using a new type of ceramic membrane reactor system was investigated. GOS are non-digestible oligosaccharides and have recently attracted interest as prebiotics. However, the composition of oligosaccharides fraction and the variability in ß-glycosidic linkages depends on the enzyme source. In the study presented below, native, physically immobilized, ß-galactosidase from Klyveromyces lactis (EC 3.2.1.23) was used as enzyme to catalyse transgalactosylation reaction to produce GOS, competed against the hydrolysis of lactose into its two component monosaccharides, glucose and galactose. To optimize GOS yielded, process conditions were varied: The average residence time of the enzyme was varied in the range of 13 to 24 min, the trans-membrane pressure (TMP) was in the range of 1 to 2 bar and the initial concentration of substrate was varied from 10 to 30 % (w/w). Regarding the conditions investigated here, the maximum oligosaccharide concentration exceeded 38% (w/w) when the average residence time was 24 min, the TMP was 2 bar and an initial lactose concentration of 30% (w/w) was adjusted.

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“…Engel et al [11] had made an investigation on membrane assisted GOS production where they had done adsorption based immobilization of enzyme ␤-galactosidase (EC 3.2.1.23) on an anion exchange polyether sulfone (PES) membrane. During their study, they had noticed around 82% lactose conversion and 24% GOS yield after 3600s (1 h) of the reaction period.…”

Section: Introductionmentioning

confidence: 99%

Feasibility study of enzyme immobilization on polymeric membrane: A case study with enzymatically galacto-oligosaccharides production from lactose

Sen

Sarkar

Gosling

et al. 2011

Journal of Membrane Science

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Membrane chromatography reactor system for the continuous synthesis of galactosyl-oligosaccharides

Cited by 21 publications

References 13 publications

Maximization of β-Galactosidase Production: A Simultaneous Investigation of Agitation and Aeration Effects

Maximization of β-Galactosidase Production: A Simultaneous Investigation of Agitation and Aeration Effects

A novel ceramic membrane reactor system for the continuous enzymatic synthesis of oligosaccharides

Feasibility study of enzyme immobilization on polymeric membrane: A case study with enzymatically galacto-oligosaccharides production from lactose

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