Feasibility study of enzyme immobilization on polymeric membrane: A case study with enzymatically galacto-oligosaccharides production from lactose

Sen, Dwaipayan; Sarkar, Ankur; Gosling, Aaron; Gras, Sally L.; Stevens, Geoffrey W.; Kentish, Sandra E.; Bhattacharya, Paramita; Barber, Andrew R.; Bhattacharjee, Chiranjib

doi:10.1016/j.memsci.2011.05.032

Cited by 51 publications

(22 citation statements)

References 34 publications

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“…The formation of monosaccharides may cause enzyme inhibition. Thus, the separation and purification of the GOS mixture is essential in the production process . In recent years, a series of REIMs has been developed for the continuous synthesis of GOS.…”

Section: The Applications Of Reimsmentioning

confidence: 99%

Immobilization of Enzymes in/on Membranes and their Applications

et al. 2019

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Enzyme membrane reactors (EMRs) are becoming increasingly interesting for application in bioconversion processes in food processing, pharmaceutics, biorefinery and wastewater treatment. Integrating the highly efficient enzymatic reaction with selectable membrane separation technology, the EMRs are able to reduce product inhibition, improve the stability of the enzyme, increase the number of reaction cycles and sustainably separate products from biotransformation solutions. Generally, in EMRs, enzymes can be either free in the solution, immobilized on an additional carrier or immobilized directly in/on a porous structure of the membrane, of which the system with the enzyme immobilized directly in/on the membrane (EIM) is a relatively simple and most widely applied method. The EIM system not only facilitates recycling of the enzymes but also in many cases additionally enhances enzyme properties such as the stability and viability. A membrane with a porous structure is usually considered as a potential carrier for enzyme immobilization. A series of immobilization methods has been developed to fix the enzymes on the membranes. The purpose of this review is to systemically summarize the immobilization methods of enzymes in/on membranes and the applications of the EIM system for biocatalysis.

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Section: The Applications Of Reimsmentioning

confidence: 99%

Immobilization of Enzymes in/on Membranes and their Applications

et al. 2019

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“…These objectives can be achieved by enzyme immobilization on solid supports, which also allows the development of continuous and sequential batch processes [7]. ␤-gal from B. circulans has been immobilized by physical adsorption on Duolite ES-762 [8] and by covalent attachment to acrylic supports [9], polymeric membranes [10], Eupergit C [11,12] and agarose modified with cyanogen bromide groups [13]. The main disadvantage of these methodologies is related with the low mechanical resistance shown by the organic supports, especially under high pressure, at which they lose their structural characteristics affecting the enzyme catalytic efficiency.…”

Section: Introductionmentioning

confidence: 99%

Improvement of thermal stability of β-galactosidase from Bacillus circulans by multipoint covalent immobilization in hierarchical macro-mesoporous silica

Bernal

Sierra

Mesa

2012

Journal of Molecular Catalysis B: Enzymatic

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“…This immobilization route requires oxidation/reduction of the polymer where the polymer carrier becomes charged and thus, the oppositely charged enzyme can interact with the conductive polymer. This system, where galactose was immobilized by electrochemical dipping, was used for development of biosensors for galactose monitoring (Shaolin 1994).…”

Section: Electrostatic Bindingmentioning

confidence: 99%

The Enzyme Immobilization: Carriers and Immobilization methods

Spasojević¹,

Prodanović²,

Pantić³

et al. 2020

JEPM

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Strategies based on the enzyme application are increasingly replacing the conventionalchemical procedures because of their efficiency, quicker performance and environmentalprotection. However, natural enzymes can rarely be used in industrysince their beneficial features cannot endure the industrial conditions. Additionaldrawbacks of natural enzymes are their inhibition by reaction products and difficultyto be removed from the reaction mixture. The most promising techniqueto substantially improve the enzyme properties, such as activity, pH, thermal andorganic-solvent stability, reusability and storage stability, in non-natural environmentsis by the enzyme immobilization. In this review different techniques usedto immobilize enzymes to inert carriers were summarized. Different materials ofboth the organic and inorganic origin were used as carriers for the enzyme immobilization.A class of new materials where the enzyme performance was enhancedby combining different classical materials and shaping in specific forms was alsosummarized.

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Feasibility study of enzyme immobilization on polymeric membrane: A case study with enzymatically galacto-oligosaccharides production from lactose

Cited by 51 publications

References 34 publications

Immobilization of Enzymes in/on Membranes and their Applications

Immobilization of Enzymes in/on Membranes and their Applications

Improvement of thermal stability of β-galactosidase from Bacillus circulans by multipoint covalent immobilization in hierarchical macro-mesoporous silica

The Enzyme Immobilization: Carriers and Immobilization methods

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