Melez Mısır Islahında In-Vivo Katlanmış Haploid Tekniğinde Kullanılan Farklı Inducer Genotiplerin Haploid İndirgeme Oranların Belirlenmesi

Abstract

ÖzBu çalışma melez mısır ıslahında in-vivo katlanmış haploid hatların elde edilmesi çalışmasında kullanılan farklı induzer genotiplerin haploid indirgeme oranlarının tespit edilmesi amacıyla 2014 yılında Doğu Akdeniz Tarımsal Araştırma Enstitüsü'nde yürütülmüştür. Çalışmada induzer olarak (haploid indirgeyici ve toz verici) RWS, RWK-76 hatları ile bu hatların melezi olan RWS X RWK-76 melezi ve Stock-6 hattı kullanılmıştır. Haploid tohum elde etmek amacıyla ana (toz alıcı) olarak, toplamda 75 farklı genotip kullanılmıştır. Bunlardan 66'sını Doğu Akdeniz Tarımsal Araştırma Enstitüsü mısır ıslah çalışmaları kapsamında elde edilen F 2 kademesindeki materyal, 9'unu ise ticari hibrit çeşitlerin açıkta tozlanması ile elde edilen populasyonlardan seçilen 9 farklı genotip oluşturmuştur. Induzer olarak kullanılan genotiplerin haploid indirgeme oranları çalışmada ana (toz alıcı) olarak kullanılan genotiplere göre değişiklik göstermiştir. Ana olarak kullanılan 75 genotipten 69'undan toplam 1463 adet haploid tohum alınmıştır. Haploid tohumların seleksiyonu renk markörüne göre yapılmıştır. En yüksek haploid tohum oranı %7.80 ile induzer olarak kullanılan RWK-76 hattından elde edilmiştir. En düşük haploid tohum oranı ise %1.28 ile induzer Stock-6 hattından alınmıştır. Yapılan çalışmada ortalama haploid tohum elde etme oranı %4.79 olarak saptanmıştır. Determination of Haploid Induction Rates of Different Inducer Lines Used for In-Vivo Double Haploid Technique in Hybrid Maize Breeding AbstractThis research was conducted to determine haploid induction rate (HIR) of different inducer lines used for in-vivo double haploid technique in hybrid maize breeding at the East Mediterranean Agricultural Research Institute. In the study RWS, RWK-76 inducer lines, their cross RWS X RWK-76 and Stock-6 were used as male parent for haploid induction. In the study 75 different F 2 genotypes as female parent were used for haploid seed production. The 66 of F 2 genotypes were selected from the corn breeding material of East Mediterranean Agricultural Research Institute, and 9 genotypes were selected from open pollinated populations of the commercial hybrid cultivars. Results of the study showed that haploid induction rate of different inducer genotypes changed depending on the female parent. It was obtained 1463 haploid seeds from 69 F 2 genotypes of total 75 F 2 genotypes. The highest haploid induction rate (7.8%) was obtained from the inducer RWK-76 line, while the lowest haploid induction rate (1.28%) was obtained from the stock-6, and average haploid induction rate (HIR) was 4.79%.

Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn

“…Cengiz (2016) reported that the highest HIR was established as 20.42% in the RWK-76 inducer line and that the lowest HIR was calculated as 17.75% in WS14. Cerit at al (2016) reported that the RWK-76 has highest HIR and the lowest one is Stock-6. Dang et al (2011) reported that the rates of putative haploids among progenies induced with the inducer lines RWK76 and RWK-76xRWS (15.7 and 15.0%, on average, respectively) were significantly lower (P<0.05) than those observed with the inducer line RWS (23.8%) for the same maternal plant material.…”

Effects of Donor x Inducer Interaction on the Success of Haploid Induction and Comparison of Haploid Seed Identification Methods in the In vivo Maternal Haploid Technique in Maize

“…Not all inducers provide the same success, and they cannot be used in harmony with every donor (female parent) genotype. In fact, the induction rates achieved in studies using different numbers of donor materials showed a wide variation ranging from 7.1% to 12.8% (Cerit et al, 2016;Zararsız et al, 2019). These results demonstrate that donors and inducers play a key role in the in vivo doubled haploid technique.…”

“…HIR values for the in vivo maternal haploid technique varied between 2.8% and 12.8% in the scientific literature (Eder & Chalyk, 2002;Prigge et al, 2012;Cerit et al, 2016;Zararsız et al, 2019). It was emphasized that the HIR varies according to the donors and inducer lines used.…”

Hyperparameter Optimization in Convolutional Neural Networks for Maize Seed Classification

“…Plant breeders have resorted to new technologies to shorten the time required to produce high-yielding and quality hybrid seeds. Techniques to obtain haploid plants provide significant advantages in shortening the seed breeding period [1]. The starting point of maize breeding programs is to produce homozygous lines that will provide hybrid diversity.…”