Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn

Abstract

This research was conducted at the breeding station of the Turkish breeding company Agromar A.Ş in the city of Bursa in Turkey during the 2013 and 2014 growing seasons. Used from within the same heterotic group crossings, 7 donor materials were obtained during the 2012 winter season in the greenhouse. The inducer line RWK-76xRWS, provided by University of Hohenheim, Germany, was used for generating haploid seeds. The donor and inducer crossing was performed during the 2013 summer season. The haploid selection and chromosome doubling were performed during the 2014 summer season. Seven donors were used for haploid induction which name are DNR1, DNR2, DNR3, DNR4, DNR5, DNR6, DNR7 respectively, from each donor different amount of ear crosses were performed (DNR1:16 ears, DNR2:10 ears, DNR3:10 ears, DNR4:12 ears, DNR5:11 ears, DNR6:13 ears, DNR7:11 ears). According to the present study, the average induction rate found ranged from 7.1 to 12.8%, and the average seedling survival rate in the greenhouse after colchicine application ranged from 57.9 to 77.6%. After transplanting to the field, 78.3-92.6% of these plants survived. As a result of this research, the chromosome doubling rate ranged from 22.5 to 48.3% depending on the donor material. These result indicates that maternal haploid selection visually is easy. Haploid induction rate (HIR) changes from donor to donor, its mean genotype and environment is effective for HIR. Average chromosome doubling rate is lower than other researchers' results, it is also effected by genotype and chromosome doubling methods.

Effects of Donor x Inducer Interaction on the Success of Haploid Induction and Comparison of Haploid Seed Identification Methods in the In vivo Maternal Haploid Technique in Maize

“…Chromosome doubling rates (CDR) are ultimately an important parameter in the in vivo maternal haploid technique. In our study, chromosome doubling rates ranged from 32.3% to 50.0% and these results were similar to the values reported in the current literature (Prasanna et al, 2012, Zararsız et al, 2019. On the other hand, it has been observed that there are significant differences between induction crosses.…”

“…Not all inducers provide the same success, and they cannot be used in harmony with every donor (female parent) genotype. In fact, the induction rates achieved in studies using different numbers of donor materials showed a wide variation ranging from 7.1% to 12.8% (Cerit et al, 2016;Zararsız et al, 2019). These results demonstrate that donors and inducers play a key role in the in vivo doubled haploid technique.…”

“…Seedling immersion method was found to be disadvantageous in terms of the number of seedlings and plants surviving both in the seedling and field stage compared to stem injection method. In other studies, the number of seedlings and plants surviving with the seedling immersion method were found to be between 57.9% and 72.9%, respectively (Zararsız et al, 2019). These results can be attributed to the adverse effects of root tip cutting on plant growth and survival rate.…”

“…From the calculations made for the first aim of the study, the haploid induction rates and the success of the doubling process according to the state of DH1 plants were determined according to the equations 1, 2, 3, 4, and 5 suggested by Zararsız et al (2019). For the second aim of the study, the evaluation statistics were calculated over the confusion matrix between the results obtained from the "Initial Classification" made by eye and the "Final Classification" performed in the field.…”

“…HIR values for the in vivo maternal haploid technique varied between 2.8% and 12.8% in the scientific literature (Eder & Chalyk, 2002;Prigge et al, 2012;Cerit et al, 2016;Zararsız et al, 2019). It was emphasized that the HIR varies according to the donors and inducer lines used.…”