Effects of Donor x Inducer Interaction on the Success of Haploid Induction and Comparison of Haploid Seed Identification Methods in the In vivo Maternal Haploid Technique in Maize

Kahrıman, Fatih; Songur, Umut; DİŞBUDAK, Abdullah; Kizik, Sezgin; VURAL, Berk

doi:10.15832/ankutbd.824114

Tarım Bilimleri Dergisi

2021

DOI: 10.15832/ankutbd.824114

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Effects of Donor x Inducer Interaction on the Success of Haploid Induction and Comparison of Haploid Seed Identification Methods in the In vivo Maternal Haploid Technique in Maize

Fatih Kahrıman¹,

Umut Songur²,

Abdullah DİŞBUDAK³

et al.

Abstract: This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record.

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Development of homozygous maize lines differing in oil and zein content using in-vivo maternal haploid technique

Kahrıman

KAHRIMAN²,

GÜZ

et al. 2022

Biotech Studies

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This study was carried out in order to develop homozygous lines that differ in grain quality from the local maize population. Twelve different local maize landraces were used as donor materials in the study. These populations were subjected to induction crossing under greenhouse conditions in September 2020 with the ADAIL-I inducer line. In September 2021, a total of twelve haploid lines were grown in greenhouse conditions. Some plant traits and some grain quality characteristics were examined. Zein protein fractions were also analyzed with SDS-PAGE analysis. The haploid induction rates (HIR) of donor materials ranged from 6.08% to 11.71%. The average HIR value of the ADAIL-I inducer line was determined as 8.20%. The average value of plant height of developed lines varied between 123 cm and 250 cm; first ear height between 54 cm and 120 cm; stem diameter between 0.7 cm and 1.2 cm; crude oil content between 2.39%, and 7.54%; oleic acid content between 15.34% and %30.98; linoleic acid content between 50.4% and 67.8%; protein content between 6.75% and 13.74%; and zein content between 4.58%, and 5.04%. Some the homozygous lines carry the desired protein bands in terms of zein fractions.

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Development of homozygous maize lines differing in oil and zein content using in-vivo maternal haploid technique

Kahrıman

KAHRIMAN²,

GÜZ

et al. 2022

Biotech Studies

Self Cite

View full text Add to dashboard Cite

show abstract

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Effects of Donor x Inducer Interaction on the Success of Haploid Induction and Comparison of Haploid Seed Identification Methods in the In vivo Maternal Haploid Technique in Maize

Abstract: This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record.

Cited by 1 publication

References 22 publications

Development of homozygous maize lines differing in oil and zein content using in-vivo maternal haploid technique

Development of homozygous maize lines differing in oil and zein content using in-vivo maternal haploid technique

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