Melatonin receptor depletion suppressed hCG-induced testosterone expression in mouse Leydig cells

Gao, Yuan; Wu, Xiaochun; Zhao, Shuqin; Zhang, Yujun; Ma, Hailong; Yang, Zhi‐gang; Yang, Wanghao; Zhao, Cheng; Wang, Li; Zhang, Quanwei

doi:10.1186/s11658-019-0147-z

Cited by 28 publications

(19 citation statements)

References 53 publications

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“…Also, relatively short-term (11 days) exposure to inhibitory photoperiods has been shown to induce local and location-specific changes in androgen receptor expression. An inverse relationship between melatonin synthesis and testosterone levels has also been reported in mouse Leydig cells and involves melatonin receptors regulating hCG-induced testosterone synthesis (Gao et al 2019). This information could be important in explaining how day length modifies gonadal steroid hormones and an interaction between testosterone and photoperiod can add to our understanding of environmental regulation of neuroendocrine function.…”

Section: The Role Of Circadian System In Male Reproductive Biologymentioning

confidence: 64%

Chronodisruption: effects on reproduction, transgenerational health of offspring and epigenome

Satı

2020

Reproduction

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The circadian system regulates the daily temporal organization in behavior and physiology, including neuroendocrine rhythms and reproduction. Modern life, however, increasingly impacts this complex biological system. Due to limitations of working with human subjects exposed to shiftwork schedules, most chronoregulation research has used rodent models. Recent publications in these model systems have emphasized the negative effects of circadian rhythm disruption on both female and male reproductive systems and fertility. Additionally, there is growing concern about the long-term effects of circadian rhythm disruptions during pregnancy on human offspring and their descendants as circadian regulation during pregnancy can also alter epigenetic programing in offspring. However, to truly know if such concerns apply to humans will require retrospective and prospective human studies. Therefore, this review will highlight the latest available evidence regarding potential effects of chronodisruption on both female and male reproductive systems. Additionally, it presents a comprehensive summary of transgenerational and epigenetic effects on adult offspring that result from maternal chronodisruption.

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Section: The Role Of Circadian System In Male Reproductive Biologymentioning

confidence: 64%

Chronodisruption: effects on reproduction, transgenerational health of offspring and epigenome

Satı

2020

Reproduction

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“…In contrast to the previous studies, in 2018 Deng et al reported that melatonin increases testosterone secretion in Leydig cell when were concurrently cultured with Sertoli cells, as well as increase in the expression of RORα gene 29 . Another study on a Leydig cancer cell line showed that suppression of melatonin receptors, MT1 and MT2, stopped the genes in the testosterone synthesis pathway 30 . These con icting results require closer attention especially considering that in our study when melatonin was concurrently administered with brin scaffold and LED green light, the genes involved in proliferation and also the genes involved in steroidogenesis were highly expressed; concluding that a 3D system would have lessened the inhibitory effects of melatonin on cell proliferation and testosterone production.…”

Section: Discussionmentioning

confidence: 99%

Three-Dimensional Scaffold, Light Emitting Diode (LED) And Melatonin Effects on Cell Proliferation and Testosterone Secretion in TM3 Leydig Cells

Alavi

Shojaei²,

Haghpanah

et al. 2021

Preprint

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Different effects of fibrin scaffold, green LED and melatonin on cell proliferation and differentiation have received extensive attentions. Testosterone, the most important sex hormone is secreted by Leydig cells to regulate spermatogenesis and many other phenomena in the body organs. The aim of the present study was to evaluate the proliferation of Leydig cells and their testosterone secretion under the influence of three-dimensional scaffold culture, green light irradiation and melatonin. The experimental groups, were TM3 cells incorporated in 3D scaffold culture, exposed to green light and also exposed to melatonin individually, in pairs and three factors together. Cell proliferation and testosterone secretion were measured after 72 hr. Cell cycle genes including PCNA, CYCLIND1, CDC2 and CDKN1B and, testosterone related genes; GATA4 and RORα were also examined. In general, three-dimensional scaffold increased Leydig cells proliferation, testosterone secretion and the expression of the related genes. On the other hand, melatonin decreased cell proliferation and testosterone synthesis. Green light did not significantly change the results but slightly decreased cell proliferation and testosterone synthesis. Combination of green light with melatonin significantly reduced the outcome while, light with scaffold increased the results. Application of light + scaffold + melatonin covered the decreasing effect of melatonin.

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“…When the melatonin receptors, especially receptor 1A, were knocked down, the decrease of the testosterone levels was observed. There was also a decrease of the steroidogenic gene expression—CYP type 11A1 and type 17A1 [ 83 ].…”

Section: Potential Drug Targets and Active Substances In Novel Thementioning

confidence: 99%

In Search of New Therapeutics—Molecular Aspects of the PCOS Pathophysiology: Genetics, Hormones, Metabolism and Beyond

Wawrzkiewicz-Jałowiecka

Kowalczyk

Trybek

et al. 2020

IJMS

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In a healthy female reproductive system, a subtle hormonal and metabolic dance leads to repetitive cyclic changes in the ovaries and uterus, which make an effective ovulation and potential implantation of an embryo possible. However, that is not so in the case of polycystic ovary syndrome (PCOS), in which case the central mechanism responsible for entraining hormonal and metabolic rhythms during the menstrual cycle is notably disrupted. In this review we provide a detailed description of the possible scenario of PCOS pathogenesis. We begin from the analysis of how a set of genetic disorders related to PCOS leads to particular malfunctions at a molecular level (e.g., increased enzyme activities of cytochrome P450 (CYP) type 17A1 (17α-hydroxylase), 3β-HSD type II and CYP type 11A1 (side-chain cleavage enzyme) in theca cells, or changes in the expression of aquaporins in granulosa cells) and discuss further cellular- and tissue-level consequences (e.g., anovulation, elevated levels of the advanced glycation end products in ovaries), which in turn lead to the observed subsequent systemic symptoms. Since gene-editing therapy is currently out of reach, herein special emphasis is placed on discussing what kinds of drug targets and which potentially active substances seem promising for an effective medication, acting on the primary causes of PCOS on a molecular level.

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Melatonin receptor depletion suppressed hCG-induced testosterone expression in mouse Leydig cells

Cited by 28 publications

References 53 publications

Chronodisruption: effects on reproduction, transgenerational health of offspring and epigenome

Chronodisruption: effects on reproduction, transgenerational health of offspring and epigenome

Three-Dimensional Scaffold, Light Emitting Diode (LED) And Melatonin Effects on Cell Proliferation and Testosterone Secretion in TM3 Leydig Cells

In Search of New Therapeutics—Molecular Aspects of the PCOS Pathophysiology: Genetics, Hormones, Metabolism and Beyond

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