Melatonin Increases the Sensitivity of Osteosarcoma Cells to Chemotherapy Drug Cisplatin

Abstract

Chemotherapy, which is one of the common treatments for osteosarcoma (OS), has many side effects and in some cases has low effectiveness due to chemoresistance, hence it is vital to study new therapies for OS. In this regard, we combined melatonin with cisplatin and evaluate their effect on MG63 OS cells. Since melatonin has anti-cancer properties, we hypothesized that its combination with cisplatin could increase the effectiveness of cisplatin. Firstly, MTT assay was used to evaluate the cell viability and cytotoxicity of cisplatin on MG63 cells and the results showed that melatonin in combination with cisplatin increases the sensitivity of MG63 cells to cisplatin. In addition, qRT-PCR results showed that the expressions of miR-181 and P53, CYLD, CBX7 and BCL2 genes change in MG63 cells after treatment with the combination of cisplatin and melatonin, so that the expression of P53, CYLD and CBX7 increased and the expression of BCL2 and miR-181b decreases significantly. Furthermore, analysis of Annexin V/FITC assay data revealed that the rate of apoptosis in MG63 OS cell line remarkably promoted after treated with cisplatin and melatonin combination. As a result, our findings show that melatonin in combination with cisplatin increases the effectiveness of cisplatin in osteosarcoma cells and this study provides a new therapeutic approach for OS.

Circulating miRNA Expression Profiles and Machine Learning Models in Association with Response to Irinotecan-Based Treatment in Metastatic Colorectal Cancer

“…Recently, Hosseini et al linked miR-181b downregulation to the increased sensitivity of osteosarcoma cells to cisplatin following the addition of melatonin to the treatment [56]. Consistent with this observation, our findings indicate that miR-181b-3p is overexpressed in the non-responders group, indicating that this may be attributed to drug resistance.…”

Melatonin and cisplatin synergistically enhance apoptosis via autophagy-dependent alteration of P53 transcription in human colorectal cancer cells

“…In the results of the tunnel analysis, it was observed that the melatonin and cisplatin treatments were therapeutically effective on HT-29 cells and increased the number of apoptotic cells. Combined administration of melatonin and cisplatin has been shown to be effective in osteosarcoma cells (16). In our study, the administration of melatonin and/or cisplatin was also found to be more effective when combined to use.…”

The Cytotoxic Effect of Benzoic Acid on Ten Different Cancer Cell Lines

“…In cases where metastasis has not occurred, the patient's survival rate increases with the removal of the tumor area through surgical intervention [36]. Osteosarcoma typically metastasizes to the lungs, exacerbating the clinical outcomes for patients [37]. The primary factors contributing to the lung metastasis rate of 80-90% in osteosarcoma include tumor-host signaling pathways, the structure of lung epithelial tissue, and innate adaptive immunity [38][39].…”

“…Lung cancer is linked to factors such as the consumption of tobacco products, air pollution, and economic inadequacy [40]. Additionally, lung cancer can occur due to the propensity of bone cancer to metastasize to the lung [37].…”