Media pH and media type can significantly affect the reliability of in vitro
 copper tolerance assessments of Pseudomonas syringae
 pv. tomato

Griffin, Karina; Brown, Philip H.; Gambley, Cherie

doi:10.1111/jam.13753

Cited by 6 publications

(2 citation statements)

References 36 publications

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“…Products of the “water disinfectants” group (Cid 2000™ and Aqua-clean ® ) displayed the highest antimicrobial capacity ( Figure 2 and Figure 5 ), followed by those of the “water acidifiers” group (Agrocid Super™Oligo, Premium acid, and Ultimate acid) ( Figure 3 and Figure 5 ), Virkon ® S, and finally those of the “Water glycerides blends” group ( Figure 4 and Figure 5 ) (CFC Floramix, FRA ® LAC34, and FRA ® Gut Balance). These results might be reflected in the susceptibility of each microorganism and/or the unique synthesis of each commercial product, as well as its capacity to modify the physicochemical character of the broth medium [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Investigation of the Antibacterial Activity of Nine Commercial Water Disinfectants, Acidifiers, and Glyceride Blends against the Most Important Poultry Zoonotic Bacteria

et al. 2023

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Identifying and monitoring the efficiency of alternative biocides that are presently used in livestock is gaining vast attention. The objective of this study was to determine, in vitro, the antibacterial activity of nine commercial water disinfectants, acidifiers, and glyceride blends against clinical isolates or reference strains of zoonotic pathogens belonging to the genera Escherichia spp., Salmonella spp., Campylobacter spp., Listeria spp., and Staphylococcus spp. For each product, the antibacterial activity was tested in concentrations ranging from 0.002 to 1.136% v/v and expressed as the minimum concentration of the product that inhibits bacterial growth (MIC). Water disinfectants Cid 2000™ and Aqua-clean® recorded MICs ranging from 0.002 to 0.142% v/v, while the lowest MICs were recorded at two strains of Campylobacter (0.002–0.004% v/v). Virkon® S displayed various MICs (0.013–0.409% w/v) and was highly effective at suppressing the growth of Gram-positive bacteria such as S. aureus (0.013–0.026% w/v). The MICs of water acidifiers (Agrocid Super™Oligo, Premium acid, and Ultimate acid) and glyceride blends (CFC Floramix, FRA®LAC34, and FRA®Gut Balance) ranged from 0.036 to 1.136% v/v, and for most of these products, MICs were closely correlated by their ability to modify the pH of the culture medium close to 5. In conclusion, most of the tested products showed promising antibacterial activity; as a result, they would be good candidates for pathogen control in poultry farms and for reducing the emergence of antimicrobial resistance. However, further in vivo studies are recommended to provide relevant information for the underlying mechanisms, as well as for the establishment of the optimal dosage scheme for each product and their possible synergies.

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Section: Discussionmentioning

confidence: 99%

In Vitro Investigation of the Antibacterial Activity of Nine Commercial Water Disinfectants, Acidifiers, and Glyceride Blends against the Most Important Poultry Zoonotic Bacteria

et al. 2023

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“…The absence of antimicrobialVERA-REYES Et Al. Sharma et al (2017) used Fe 3 O 4 NPs that had a high capacity of reduction, and ferrite was found to inhibit the in vitro growth of F. oxysporum, decreasing at the same time the incidence and severity of this fungal strain in wild plants of Capsicum genus.Susceptibility between bacterial strains assayed and NP treatments could be linked to the fact that P. syringae is copper-tolerant through mechanisms of proteins expression(Griffin, Brown, & Gambley, 2018). However, the NPs are able to be oxidized, reduced, or dissolved and are cytotoxic and genotoxic for cells(Auffan, Rose, Wiesner, & Bottero, 2009).…”

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In vitro antimicrobial effect of metallic nanoparticles on phytopathogenic strains of crop plants

Vera‐Reyes

Esparza‐Arredondo²,

Lira-Saldívar³

et al. 2019

Journal of Phytopathology

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In this research, the in vitro antimicrobial effect of zinc oxide (ZnO), copper oxide (CuO) and iron oxide (Fe2O3) nanoparticles (NPs)—with average sizes of 20, 46 and 30 nm, respectively—on the root rot disease caused by the fungus Fusarium oxysporum and on blight disease caused by the fungus Alternaria solani were studied. Also, bacterial diseases caused by Clavibacter michiganensis and Pseudomonas syringae that infects a wide range of plant species were assessed. Different concentrations of NPs (0, 100, 250, 500, 700 and 1,000 mg/L) were prepared on PDA agar or King's B medium in a complete randomized design with four replicates. According to the results, ZnO NPs exhibited an outstanding inhibitory effect against fungi and bacteria strains. The above results were associated with the smaller particle size. Fungi strains showed a differential sensitivity depending on the kind of NPs used. A. solani showed the highest sensitivity to ZnO NPs at 1,000 mg/L (99%), followed by CuO NPs at the same dose (95%). Fe2O3 NPs at all evaluated doses had no inhibitory effects on the mycelia growth of this strain, although F. oxysporum revealed greater effectiveness of the CuO NPs (96%) compared with ZnO NPs since it only inhibited 91% of the mycelial growth. The antibacterial activity was studied through optical density. C. michiganensis was found to be more sensitive to ZnO NPs because a lesser dose (700 mg/L) was required to reduce the bacterial growth (90%); in comparison, P. syringae required a dose of 1,000 mg/L to inhibit its growth (67%). CuO NPs displayed the smallest growth inhibition against the bacteria strains analysed. The antimicrobial effect of the metallic NPs that were assayed increased with higher doses.

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Genetic basis of copper-tolerance in Australian Pseudomonas syringae pv. tomato

Griffin

Campbell²,

Gambley³

2019

Australasian Plant Pathol.

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Media pH and media type can significantly affect the reliability of in vitro copper tolerance assessments of Pseudomonas syringae pv. tomato

Abstract: Methodology presented in the study can be extrapolated to copper tolerance testing for other pathogenic plant bacteria, particularly other pseudomonads.

Cited by 6 publications

References 36 publications

In Vitro Investigation of the Antibacterial Activity of Nine Commercial Water Disinfectants, Acidifiers, and Glyceride Blends against the Most Important Poultry Zoonotic Bacteria

In Vitro Investigation of the Antibacterial Activity of Nine Commercial Water Disinfectants, Acidifiers, and Glyceride Blends against the Most Important Poultry Zoonotic Bacteria

In vitro antimicrobial effect of metallic nanoparticles on phytopathogenic strains of crop plants

Genetic basis of copper-tolerance in Australian Pseudomonas syringae pv. tomato

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