Genetic basis of copper-tolerance in Australian Pseudomonas syringae pv. tomato

Griffin, Karina; Campbell, Paul M; Gambley, Cherie

doi:10.1007/s13313-019-00646-y

Cited by 2 publications

(2 citation statements)

References 60 publications

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“…The cytotoxicity of copper has been exploited in agricultural systems for bacterial and fungal pathogen management for over 100 years [103]. However, since the mid-1980s there have been increasing reports of copper-tolerance in a wide range of bacterial species important to the agricultural environment, including Pseudomonas syringae, Xanthomonas campestris, Xanthomonas arboricola, Xanthomonas vesicatoria and Erwinia amylovora, among others [77,98,99,104]. In the case of P. syringae, copper tolerant strains have been isolated from kiwifruit, apple, tomato, pepper, plum, mango, sweet cherry and snap bean, from cultures in Asia-Pacific, Europe, Africa, North and South America [77].…”

Section: Copper Resistance Mechanismsmentioning

confidence: 99%

“…In this context, phylogenetic analysis indicates that individual plasmids of the PT23A family (carrying genetic copper-tolerance determinants) have been transferred between different P. syringae pathovars [108,109]. However recent findings suggests that the copABCD and copR/S operons may be located on either plasmid or chromosomal DNA, depending on the isolate studied [104]. Additional copper-tolerance mechanisms in P. syringae continue to be discovered [109,110].…”

Section: Copper Resistance Mechanismsmentioning

confidence: 99%

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Phytopathogenic Pseudomonas syringae as a Threat to Agriculture: Perspectives of a Promising Biological Control Using Bacteriophages and Microorganisms

et al. 2023

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Pseudomonas syringae is a Gram-negative bacterium that infects a wide range of plants, causing significant economic losses in agricultural production. The pathogen exhibits a high degree of genetic and phenotypic diversity, which has led to the classification of P. syringae strains into different pathovars based on their host range and disease symptoms. Copper-based products have traditionally been used to manage infections in agriculture, but the emergence of copper-resistant strains has become a significant concern. Biological control is a promising strategy to manage P. syringae, as it offers an environmentally friendly and sustainable approach to disease management. The review includes an overview of the biology and epidemiology of P. syringae, and of the mechanisms of action of various biological control agents, mainly microorganisms (antagonistic bacteria, and fungi) and bacteriophages. Specifically, this review highlights the renewed interest in bacteriophages (bacteria-infecting viruses) due to their advantages over other eco-friendly management methods, thanks to their bactericidal properties and potential to target specific pathogenic bacteria. The potential benefits and limitations of biological control are also examined, along with research directions to optimize the use of this approach for the management of P. syringae.

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Section: Copper Resistance Mechanismsmentioning

confidence: 99%

Section: Copper Resistance Mechanismsmentioning

confidence: 99%

Phytopathogenic Pseudomonas syringae as a Threat to Agriculture: Perspectives of a Promising Biological Control Using Bacteriophages and Microorganisms

et al. 2023

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In planta interactions of a novel bacteriophage against Pseudomonas syringae pv. tomato

Skliros

Papazoglou

Gkizi

et al. 2023

Appl Microbiol Biotechnol

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The biology and biotechnology of bacteriophages have been extensively studied in recent years to explore new and environmentally friendly methods of controlling phytopathogenic bacteria. Pseudomonas syringae pv. tomato (Pst) is responsible for bacterial speck disease in tomato plants, leading to decreased yield. Disease management strategies rely on the use of copper-based pesticides. The biological control of Pst with the use of bacteriophages could be an alternative environmentally friendly approach to diminish the detrimental effects of Pst in tomato cultivations. The lytic efficacy of bacteriophages can be used in biocontrol-based disease management strategies. Here, we report the isolation and complete characterization of a bacteriophage, named Medea1, which was also tested in planta against Pst, under greenhouse conditions. The application of Medea1 as a root drenching inoculum or foliar spraying reduced 2.5- and fourfold on average, respectively, Pst symptoms in tomato plants, compared to a control group. In addition, it was observed that defense-related genes PR1b and Pin2 were upregulated in the phage-treated plants. Our research explores a new genus of Pseudomonas phages and explores its biocontrol potential against Pst, by utilizing its lytic nature and ability to trigger the immune response of plants. Key points • Medea1 is a newly reported bacteriophage against Pseudomonas syringae pv. tomato having genomic similarities with the phiPSA1 bacteriophage • Two application strategies were reported, one by root drenching the plants with a phage-based solution and one by foliar spraying, showing up to 60- and 6-fold reduction of Pst population and disease severity in some cases, respectively, compared to control • Bacteriophage Medea1 induced the expression of the plant defense-related genes Pin2 and PR1b

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Genetic basis of copper-tolerance in Australian Pseudomonas syringae pv. tomato

Cited by 2 publications

References 60 publications

Phytopathogenic Pseudomonas syringae as a Threat to Agriculture: Perspectives of a Promising Biological Control Using Bacteriophages and Microorganisms

Phytopathogenic Pseudomonas syringae as a Threat to Agriculture: Perspectives of a Promising Biological Control Using Bacteriophages and Microorganisms

In planta interactions of a novel bacteriophage against Pseudomonas syringae pv. tomato

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