Mechanistic target of rapamycin inhibition with rapamycin induces autophagy and correlative regulation in white shrimp (<i>Litopenaeus vannamei</i>)

Li, Xinwei; Wang, Mengqiang; Sun, Guo-Qiong; Wang, Baojie; Jiang, Keyong; Shao, Jianchun; Qi, Cancan; Zhao, Wei; Han, Shuyi; Liu, Mei; Wang, Lei

doi:10.1111/anu.12688

Cited by 12 publications

(6 citation statements)

References 67 publications

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“…The IGF-I and TOR levels have been evaluated to correlate with growth in different aquatic animals, such as European sea bass (Dicentrarchus labrax) (Carnevali et al, 2006), tilapia (O. niloticus) (Yan et al, 2013), juvenile turbot (S. maximus) (Wang et al, 2016), and pacific white shrimp (Liu et al, 2018). In this study, it was observed that hybrid grouper fed with different levels of HFP obtained a significantly higher total crude protein level compared to the control.…”

Section: Discussionmentioning

confidence: 88%

Effects of Lower Fishmeal With Hydrolyzed Fish Protein Powder on the Growth Performance and Intestinal Development of Juvenile Pearl Gentian Grouper (Epinephelus fuscoguttatus ♀ and Epinephelus lanceolatus ♂)

Hlordzi¹,

Wang²,

Li³

et al. 2022

Front. Mar. Sci.

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The effect of hydrolyzed fish protein powder (HFP) on the growth, intestinal development, gene mRNA expression, and enzyme activity in the intestine and liver of juvenile hybrid grouper (Epinephelus fuscoguttatus ♀ and Epinephelus lanceolatus ♂) was assessed after an 8-week feeding trial. Seven isonitrogenous (50%) and isolipidic (9%) diets were fed to hybrid grouper with 0% (CT), 1% (H1), 1.5% (H2), 2% (H3), 2.5% (H4), 3% (H5), and 4% (H6) HFP. No significant difference (p > 0.05) in weight gain rate (WGR), specific growth rate (SGR), feed conversion ratio (FCR), and survival rate (SR) was observed in all the groups. The crude protein content in the H6 group was significantly higher than in the other groups (p < 0.05). Intestinal lipase and trypsin activity were significantly higher in H3 and H5 groups (p < 0.05). In the serum, superoxide dismutase (SOD) activity was significantly higher in H5 and H6 groups, while malondialdehyde (MDA) activity was lower (p < 0.05) compared to other treatments. Insulin-like growth factor (IGF-I) and target of rapamycin (TOR) mRNA expression levels in the intestine and muscle were significantly higher in the H2 group and H1 group (p < 0.05), respectively. The most abundant intestinal bacteria found at the genus level are Acinetobacter, Vibrio, and Flavobacteriaceae. The villus was significantly longer in hybrid grouper fed with different levels of HFP compared to the control, and fish in the H2 group had thicker intestinal muscle compared to the other groups (p < 0.05). In conclusion, the addition of HFP to the low fishmeal (FM) diets of juvenile grouper improved the intestinal development and increased the levels of intestinal digestive enzymes.

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Section: Discussionmentioning

confidence: 88%

Effects of Lower Fishmeal With Hydrolyzed Fish Protein Powder on the Growth Performance and Intestinal Development of Juvenile Pearl Gentian Grouper (Epinephelus fuscoguttatus ♀ and Epinephelus lanceolatus ♂)

Hlordzi¹,

Wang²,

Li³

et al. 2022

Front. Mar. Sci.

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“…Mechanistic target of rapamycin (mTOR) represents a critical regulatory protein involved in protein production, cell growth/proliferation, autophagy, lysosomal function and metabolic pathways, and is considered a central regulator of cell growth 23 . It is admitted that mTOR is a primary suppressor of autophagy, which acts by controlling essential effectors at the transcriptional and post-transcriptional levels, and by directly phosphorylating the autophagy machinery 24 .…”

Section: Discussionmentioning

confidence: 99%

Qi Dan Li Xin pill improves chronic heart failure by regulating mTOR/p70S6k-mediated autophagy and inhibiting apoptosis

Shi

Huang

et al. 2020

Sci Rep

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Myocardial remodeling represents a key factor in chronic heart failure (CHF) development, and is characterized by chronic death of cardiomyocytes. Cardiac function changes may be attributed to inflammation, apoptosis and autophagy. This study assessed the effects of Qi Dan Li Xin Pill (QD) on heart function, inflammatory factors, autophagy and apoptosis in cardiac remodeling in CHF rats upon myocardial infarction (MI) induction.Male SD rats underwent a sham procedure or left anterior descending coronary artery (LADCA) ligation, causing MI. Twenty-eight days after modeling, the animals were treated daily with QD, valsartan and saline for 4 weeks. Echocardiography after 4 weeks of drug intervention revealed substantially improved left ventricular remodeling and cardiac function following QD treatment. As demonstrated by decreased IL-1β, IL-6 and TNF-α amounts, this treatment also inhibited the apoptotic process and protected the viability of the myocardium. These outcomes may be attributed to enhanced autophagy in cardiomyocytes, which further reduced pro-inflammatory and pro apoptotic effects. This process may be achieved by QD regulation of the mTOR/P70S6K signaling pathway, suggesting that the traditional Chinese medicine Qi Dan Li Xin pill is effective in heart protective treatment, and is worth further investigation.

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“…Additionally, it has also been found that sustained molting steroid production and secretion in the molting glands of blackback land crab (Gecarcinus Lateralis) [17] and green crab (Carcinus maenas) [18] relied on mTOR-dependent protein synthesis [19]. In Litopenaeus vannamei, the inhibitor rapamycin inhibited the mTOR signaling pathway, significantly changed the expression of many autophagy-related genes, and significantly increased the ratio of LC3II/LC3I [20]. In Crassostrea gigas, 35 autophagy-associated proteins were first identified, followed by analysis of the expression and localization of microtubule-associated proteins 1A/1B-light chain 3 (LC3) and mTOR in different tissues of oysters [21].…”

Section: Introductionmentioning

confidence: 99%

mTOR Plays a Conserved Role in Regulation of Nutritional Metabolism in Bivalve Sinonovacula constricta

Zhou

Li²,

Liao

et al. 2023

JMSE

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The mammalian target of rapamycin (mTOR) has been shown to play a central role in regulating cell growth and metabolism. However, little is known about the function of mTOR in nutrient metabolism in bivalve mollusks. In this study, the role of mTOR in the regulation of nutrient metabolism was investigated in Sinonovacula constricta. First, the activation of mTOR was assayed after starvation and refeeding. Afterwards, the role of mTOR in the regulation of nutrient metabolism was investigated using an activator (MHY1485) or inhibitor (rapamycin) of mTOR. The open reading frame of the S. constricta mTOR is 7416 bp in length and encodes a polypeptide consisting of 2471 amino acids. The mTOR amino acid sequence of S. constricta was highly conserved when compared with other species and had a close evolutionary relationship with the TOR proteins of Crassostrea gigas and Lingula anatine. mTOR was expressed in the intestine, exhalent siphon, labial palppus, muscle, inhalent siphon, gill, mantle, digestive land, and gonad tissue of S.constricta, with the highest expression in muscle. During starvation, the level of phosphorylated mTOR protein was relatively low, and the ratio of LC3II/LC3I protein and the AMPKα mRNA level significantly increased with the increase in starvation time. After feeding, the level of phosphorylated mTOR protein increased from 0.13 to 0.56, and the ratio of LC3II/I protein and AMPKα mRNA level decreased from 1.17 to 0.38. MHY1485 significantly increased the level of phosphorylated 4E-BP1 and significantly decreased the ratio of LC3II/I proteins. Furthermore, MHY1485 significantly increased the mRNA level of the glucose metabolism-related gene glucokinase (GK), significantly decreased the mRNA expression of the G6P gene, and significantly increased the mRNA expression of the lipid synthesis-related genes sterol-regulatory element-binding protein (SREBP) and stearoyl-CoA desaturase (SCD). Rapamycin significantly reduced the level of phosphorylated 4E-BP1 and the mRNA expression of mTOR, and the expression level of phosphorylated 4EBP1 decreased from 0.97 to 0.28. Meanwhile, it also significantly reduced the mRNA expression of glucose metabolism-related genes GK, pyruvate kinase (PK), glucose transporter 1 (GLUT1), and G6P, as well as lipid synthesis-related genes SCD and acetyl-CoA carboxylase (ACC). These results indicate a conserved role of mTOR in regulating nutritional metabolism, including glucose metabolism, lipid synthesis, and autophagy in S. constricta.

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Mechanistic target of rapamycin inhibition with rapamycin induces autophagy and correlative regulation in white shrimp (Litopenaeus vannamei)

Cited by 12 publications

References 67 publications

Effects of Lower Fishmeal With Hydrolyzed Fish Protein Powder on the Growth Performance and Intestinal Development of Juvenile Pearl Gentian Grouper (Epinephelus fuscoguttatus ♀ and Epinephelus lanceolatus ♂)

Effects of Lower Fishmeal With Hydrolyzed Fish Protein Powder on the Growth Performance and Intestinal Development of Juvenile Pearl Gentian Grouper (Epinephelus fuscoguttatus ♀ and Epinephelus lanceolatus ♂)

Qi Dan Li Xin pill improves chronic heart failure by regulating mTOR/p70S6k-mediated autophagy and inhibiting apoptosis

mTOR Plays a Conserved Role in Regulation of Nutritional Metabolism in Bivalve Sinonovacula constricta

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