Progress has been made in establishing a genetic transformation model for Laminaria japonica (Phaeophyta, Laminariales). The model includes introduction of foreign genes by biolistic bombardment, use of promoter SV 40 to drive gene expression, algal regeneration by parthenogenesis and selection by chloramphenicol or hygromycin.
Mechanistic target of rapamycin (mTOR) is a central regulator of cell growth and metabolism including autophagy. The mTORC1 signalling pathway is a major regulator of autophagy in response to nutrient levels. The drug rapamycin induces autophagy in various cell types and species by specifically inhibiting the activity of mTOR. To identify the genes and correlative regulatory pathways involved in cell metabolism and autophagy of Litopenaeus vannamei, we analysed the transcriptome of shrimps treated with rapamycin. We found that the expression of some genes related to autophagy was significantly changed. To further explore the autophagy regulatory mechanism, the concentration and phosphorylation of related proteins were analysed by Western blot. We found that treatment with the rapamycin can depress phosphorylating of ATG1 and promote dephosphorylating of ATG13. There are some heat‐shock protein genes and genes involving apoptosis, function of lysosome and feedback regulation of mTOR signalling path differently expressed. The relationship between them and autophagy induced by inhibiting mTOR is also discussed. Evidences show the complicated positive and negative feedback loops controlling autophagy crustaceans are scarce. Our study verifies and contributes to the current understanding of autophagy induced by mTOR in crustacean, providing an abundant source for the identification of novel genes.
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