Mechanisms of somatic embryogenesis in cell cultures: Physiology, biochemistry, and molecular biology

Komamine, Atsushi; Kawahara, Ryoichi; Matsumoto, Mitsuyo; Sunabori, Satoshi; Toya, Takashi; Fujiwara, Akio; Tsukahara, Masayoshi; Smith, Jane A.; Ito, Miyuki; Fukuda, Hiroo; Nomura, Koji; Fujimura, Tatsuhito

doi:10.1007/bf02632185

Cited by 97 publications

(77 citation statements)

References 17 publications

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“…Auxin is necessary to promote cell growth in an explant (hence to generate a cell line) and is also necessary to generate totipotent cells (hence an embryogenic cell line). In order to have an actual embryo, the formation of a PEM is a prerequisite (10). When embryos reach the globular stage, auxin will start being synthesized by a few cells, others will respond to it and generation of diversity in the form of different tissues will ensue.…”

Section: Discussionmentioning

confidence: 99%

Modulation of Auxin-Binding Proteins in Cell Suspensions

Loschiavo

Filippini²,

Cozzani³

et al. 1991

Plant Physiol.

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This paper shows that the level of 2,4-dichlorophenoxyacetic acid (2,4-D) in the medium determines the level of auxin-binding proteins in the membranes of carrot, Daucus carota, cells grown in suspension. This induction takes slightly more than 2 hours to complete and can be elicited by natural as well as synthetic auxins. The auxin binding sites thus generated, which are pronase-sensitive, bind 2,4-D, indoleacetic acid, and naphthaleneacetic acid (NAA) equally well. However both a-and f,-NAA bind, whereas only a-NAA is effective in the inductive process. Cells committed to embryogeny (proembryogenic masses) do not respond to auxin, i.e. their level of auxin-binding proteins remains very low, and they do not seem to synthesize the hormone, as indicated by inhibitor studies. Sensitivity to, and production of, auxin, begins when the embryo becomes polarized, i.e. at postglobular stage.

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Section: Discussionmentioning

confidence: 99%

Modulation of Auxin-Binding Proteins in Cell Suspensions

Loschiavo

Filippini²,

Cozzani³

et al. 1991

Plant Physiol.

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“…That indicates that the simple reduction of medium auxins is sufficient to complete the guava embryo development, which is particular of somatic embryogenic processes, as postulated by Komanine et al (1992).…”

Section: Resultsmentioning

confidence: 84%

Induction of somatic embryogenesis in immature seeds of guavatree cv. Paluma

Moura

Motoike

2009

Rev. Bras. Frutic.

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The biotechnological techniques may help solve many problems of guava culture, such as the high perishability of fruits. Somatic embryogenesis can generate highly multiplicative cell cultures and with high regenerative potential, serving as basis for genetic transformation. The aim of this work was to obtain somatic embryogenesis of guava (Psidium guajava L.) cv. Paluma. Immature seeds were used, and they were inoculated in MS environment containing 400 mg L-1 of L-glutamine, 100 mg L-1 myo-inositol, 60 g L-1 sucrose, 100 mg L-1 ascorbic acid and supplemented with different types and concentrations of growth regulators. Embryogenic callus appeared after 37 days of culture in an environment containing 1.0 mg L-1 2.4-D + 2.0 mg L-1 2-ip, in 7% of the explants. After 65 days of culture, the treatment containing 0.5 mg L-1 CPA showed 20% of explants with direct embryos, while the treatment with 1 mg L-1 had 14% of explants with direct embryos and 7% of explants with embryogenic callus. In 66.6% of embryos regenerated with 0.5 mg L¹ CPA there was the formation of secondary embryos. The use of IASP and BAP, aiming embryogenesis proliferation, led to an increase in the cellular proliferation, but calli apparently lost their embryogenic potential.

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“…Some genotypes exhibit high germination capacity, while others are either recalcitrant or irresponsible 44,45 . Specific genes are probably involved in each stage of plant development or regeneration 46 (dedifferentiation, acquisition of competence, and induction).…”

Section: Discussionmentioning

confidence: 99%

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Thawaro¹,

Te-chato²

2010

ScienceAsia

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Nutritional requirements for germination of mature zygotic embryos of four genotypes of Elaeis guineensis Jacq. Dura × Pisifera were assessed. A factorial experiment was conducted with four genotypes from separate crossing events and three culture media to show Interactions between genotypes and culture media on seedling germination (p < 0.05). The highest frequency of normal seedling germination was facilitated by 1/2 Murashige and Skoog (MS) medium (29.2%), followed by MS (15.0%) and Blaydes (12.9%). Speed of shoot formation followed a similar trend. Roots were induced most rapidly in 1/2 MS medium, followed by Blaydes and then MS. Root induction was similar in 1/2 MS medium (2.29%) and Blaydes medium (1.25%) but only significantly higher than in MS (0.00%).

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Mechanisms of somatic embryogenesis in cell cultures: Physiology, biochemistry, and molecular biology

Cited by 97 publications

References 17 publications

Modulation of Auxin-Binding Proteins in Cell Suspensions

Modulation of Auxin-Binding Proteins in Cell Suspensions

Induction of somatic embryogenesis in immature seeds of guavatree cv. Paluma

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