Mechanisms of Cardiotoxin Iii‐induced Apoptosis in Human Colorectal Cancer Colo205 Cells

Tsai, Chia-Houng; Yang, Sheng-Huei; Chien, Ching-Ming; Lu, Mei‐Chin; Lo, Chao‐Sheng; Lin, Yi‐Hsiung; Hu, Xiu-Wei; Lin, Shinne‐Ren

doi:10.1111/j.1440-1681.2006.04334.x

Cited by 43 publications

(19 citation statements)

References 37 publications

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“…Western blotting demonstrated that PSⅠ treatment dose-dependently up-regulated the expression of Bax and down-regulated the expression of Bcl-2, indicating that the modulation of Bcl-2 and Bax was critical for PSⅠ-induced apoptosis of SGC7901 cells. Indeed, Bax accelerated the release of the apoptosis-inducing factor cytochrome c from the mitochondria, thus activating the caspase cascade [28][29] .…”

Section: Discussionmentioning

confidence: 99%

Paris saponin I induces G2/M cell cycle arrest and apoptosis in human gastric carcinoma SGC7901 cells

Xiao

Dai

et al. 2011

J. Huazhong Univ. Sci. Technol. [Med. Sci.]

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The aim of this study was to investigate the effect of Paris saponin I (PS I) on human gastric carcinoma cell growth and apoptosis and to explore the potential mechanisms. The proliferation of SGC7901 cells was monitored by the MTT cell viability assay, while the nuclear morphology of apoptotic cells was assessed by Hoechst 33258 staining. Flow cytometry was performed to analyze the cell cycle progression of propidium iodide (PI)-stained SGC7901 cells and the apoptotic rate of annexin V/PI-stained cells. Western blotting was used to examine the expression of several cell cycle proteins, including cyclin B1 and Cdk1, and the apoptosis-regulated proteins Bcl-2, Bax, cytochrome c, procaspase-9, and procaspase-3. The MTT assay demonstrated that PS I could induce significant dose- and time-dependent inhibition of SGC7901 cell proliferation. Marked morphological changes, including condensation of chromatin, nuclear fragmentation and apoptotic bodies were clearly shown on Hoechst 33258 staining. PSI treatment also resulted in the disruption of the cell cycle at G₂/M and the induction of apoptosis. Following PSI treatment, the cell cycle-related proteins cyclin B1 and Cdk1 were down-regulated. Expression of the pro-apoptotic protein Bax was increased, while anti-apoptotic protein Bcl-2 decreased. PSI treatment resulted in elevated cytoplasmic cytochrome c and activation of the apoptotic proteases caspase-9 and caspase-3. These data indicate that PS acts as an inhibitor of proli I feration in SGC7901 cells by inducing cell cycle arrest and mitochondria-dependent apoptosis. PSI is a potential therapeutic agent against human gastric carcinoma.

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Section: Discussionmentioning

confidence: 99%

Paris saponin I induces G2/M cell cycle arrest and apoptosis in human gastric carcinoma SGC7901 cells

Xiao

Dai

et al. 2011

J. Huazhong Univ. Sci. Technol. [Med. Sci.]

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“…Numerous heat-stable neurotoxins and cardiotoxins -obtained from venoms of different cobra species -were already detected and structurally studied (3). Some of them presented anticancer potential, some antinociceptive properties and others even inhibited bone resorption (4,18,(20)(21)(22).…”

Section: Discussionmentioning

confidence: 99%

Proteomic characterization of the thermostable toxins from Naja naja venom

Binh¹,

Thanh²,

Chi³

2010

J. Venom. Anim. Toxins incl. Trop. Dis

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Naja naja snake venom presents abundant thermostable peptides. Many of them possess useful pharmacological activity that may be employed for drug development. For the proteomic characterization of such toxins, in the present study, Naja naja venom solution was heated up to 100°C for 10, 30, 60, 120, 180 and 300 minutes and protein fractions of non-heated and heated venom were analyzed by twodimensional nano-liquid chromatography coupled online with tandem mass spectrometry. After heating for 300 minutes, a total of 32 peptides were still detected in the supernatant. The identified peptides belong to the following groups: cardiotoxins, neurotoxins and cytotoxins. It was found that thermostable peptides are able to preserve their analgesic activity after a long heating time in formalin test. Mice injected with 15 µg/g of 60-minute heated venom or with 25 µg/g of 300-minute heated venom revealed even a better analgesic activity than those treated with lidocaine.

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“…Although some anticancer drugs such as doxorubicin, anthracyclines, and trastuzumab have the well-known cardiotoxicity [10, 11], CTXIII was found to exhibit a variety of bioactivities with anticancer potential. For example, we previously found that CTXIII inhibits the cellular proliferation and induces apoptosis of various cancer cells, including breast cancer [12], leukemia cells [13], colorectal cancer [14], and oral cancer [15, 16]. …”

Section: Introductionmentioning

confidence: 99%

Cardiotoxin III Inhibits Proliferation and Migration of Oral Cancer Cells through MAPK and MMP Signaling

Yen

Liang

Han

et al. 2013

The Scientific World Journal

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Cardiotoxin III (CTXIII), isolated from the snake venom of Formosan cobra Naja naja atra, has previously been found to induce apoptosis in many types of cancer. Early metastasis is typical for the progression of oral cancer. To modulate the cell migration behavior of oral cancer is one of the oral cancer therapies. In this study, the possible modulating effect of CTXIII on oral cancer migration is addressed. In the example of oral squamous carcinoma Ca9-22 cells, the cell viability was decreased by CTXIII treatment in a dose-responsive manner. In wound-healing assay, the cell migration of Ca9-22 cells was attenuated by CTXIII in a dose- and time-responsive manner. After CTXIII treatment, the MMP-2 and MMP-9 protein expressions were downregulated, and the phosphorylation of JNK and p38-MAPK was increased independent of ERK phosphorylation. In conclusion, CTXIII has antiproliferative and -migrating effects on oral cancer cells involving the p38-MAPK and MMP-2/-9 pathways.

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Mechanisms of Cardiotoxin Iii‐induced Apoptosis in Human Colorectal Cancer Colo205 Cells

Cited by 43 publications

References 37 publications

Paris saponin I induces G2/M cell cycle arrest and apoptosis in human gastric carcinoma SGC7901 cells

Paris saponin I induces G2/M cell cycle arrest and apoptosis in human gastric carcinoma SGC7901 cells

Proteomic characterization of the thermostable toxins from Naja naja venom

Cardiotoxin III Inhibits Proliferation and Migration of Oral Cancer Cells through MAPK and MMP Signaling

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