Mechanisms Involved in Electrically-induced Responses of Rat Seminal Vesicles

Abstract

Contractile responses of rat isolated seminal vesicle were elicited by electrical field stimulation (EFS, 10 Hz, 1 ms, 40 V for 5 s), noradrenaline (1 x 10(-5) M) and carbachol (1 x 10(-5) M). Guanethidine (2 x 10(-5)-5 x 10(-4) M) progressively reduced the contraction induced by EFS and carbachol to 24 +/- 2 and 10 +/- 2%, respectively, at the highest concentration (n = 6), while potentiating noradrenaline contraction to a maximum of 154 +/- 14% at 2 x 10(-5) M (n = 6). Prazosin (1 x 10(-6) M) and atropine (2.5 x 10(-7) M) completely abolished the response to the corresponding agonist and each reduced the response to EFS to 64 +/- 8 and 61 +/- 3%, respectively (n = 6). In the presence of both atropine and prazosin a small contraction to EFS remained (14 +/- 4%, n = 6), which is unlikely to be due to ATP, since exogenous ATP did not induce a contractile response and had an inhibitory effect on EFS-induced responses. Clonidine (1.25 x 10(-5) M) completely blocked responses to noradrenaline and reduced the responses to EFS to 68 +/- 7% (n = 6). However, when both the adrenergic and cholinergic components of EFS were blocked by prazosin and atropine, clonidine potentiated the remaining response to EFS (323 +/- 82%, n = 4). Yohimbine (1 x 10(-5) M) blocked the response to noradrenaline and reduced the response to EFS to 37 +/- 5% (n = 6) while the carbachol response was unaffected.(ABSTRACT TRUNCATED AT 250 WORDS)

Ejaculatory dysfunction caused by the new α1‐blocker silodosin: A preliminary study to analyze human ejaculation using color Doppler ultrasonography

“…In addition to the retrograde inflow attributable to insufficient closure of the bladder neck, insufficient contraction of the seminal vesicles may also occur under silodosin administration. It has already been reported that α 1A receptors are present in the spermatic ducts and seminal vesicles 13–15 . The findings of the present study are consistent with the report that contraction of the seminal vesicles is involved in ejaculation.…”

Atropine-Induced Inhibition of Sperm and Semen Transport Impairs Fertility in Male Rats

“…There were no treatment-related histopathological changes of the seminal vesicle, indicating that atropine itself did not affect the secretion of seminal vesicle fluid. Therefore, the increased seminal vesicle weights and decreased copulatory plug weights in the atropine group were considered to be resulted from inhibition of contraction of the seminal vesicle during the process of emission, which is one of the pharmacological effects of the muscarinic receptor antagonists (Sadraei et al, 1995). It has been reported (Gokhale et al, 1996) that contractile responses of rat isolated seminal vesicles induced by electrical field stimulation involve participation of a noradrenergic as well as a cholinergic mechanism, which also supports the results of the present study.…”

A novel method of seminal vesicle preparation in isolated seminal vesicle experiments in the rat: ring preparation

“…3,9,[21][22][23] Also, the frequency-dependent contractions of SV, elicited by the EFS, have been reproduced in a few experiments, which occur through neurotransmitters including acetylcholine and adenosine triphosphate. 10,[24][25][26] The results of this study showed that the proportion of tissues responding to the drugs and EFS was greater for the rings than the strips. When evaluating the methodological efficacy, we focused not on the absolute response magnitude in a responding tissue but the response rate.…”