Atropine-Induced Inhibition of Sperm and Semen Transport Impairs Fertility in Male Rats

Sato, Takahiro; Ban, Yoshiki; Uchida, Motoyuki; Gondo, E.; Yamamoto, Masahiro; Sekiguchi, Yoshiko; Sakaue, Akiko; Kemi, Masayuki; Nakatsuka, Toshio

doi:10.2131/jts.30.207

Cited by 13 publications

(11 citation statements)

References 13 publications

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“…One male and three female rats were housed together in a single cage for mating. Pregnancy was confirmed by observing vaginal plug in the morning after keeping the female and male rats together (Sato et al, 2005). Rats were monitored at 8 h interval to observe the time they deliver (Dhungel et al, 2006).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of spermatogenesis in prepubertal albino rats with date palm pollen supplement

Salman¹,

Ahmad²,

Hina³

et al. 2014

Afr. J. Pharm. Pharmacol.

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It is suggested that Date Palm Pollen (DPP) increases the concentration of testosterone, follicle stimulating hormone, and luteinizing hormone in rats. We therefore planned this study to examine the effect of DPP on spermatogenesis using prepubertal rats. 4-day old rats were randomly divided into four groups of 12 pups each (Control I, Experimental I, Control II, and Experimental II). Experimental groups I and II were given DPP suspension in a single oral dose of 120 mg/kg daily for 18 and 35 days, respectively. Pups were sacrificed on days 22 and 39 post-natally. Testes were removed for microscopic studies, and spermatogenesis was assessed by a method which depended upon scoring 'cross sectional' profiles of seminiferous tubules according to Johnsen's criterion. There was no statistically significant difference in Control and Experimental groups I. However Pups of the treated group II, who received DPP for 35 days, showed statistically significant increase in seminiferous tubules containing spermatozoa, resulting in a higher Johnsen Score (Control group II: 6.28 ± 0.21, Experimental group II: 8.06 ± 1.21). This implied that spermatogenesis was present at an advanced stage in the Experimental group as compared to the Control group of animals.

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Section: Methodsmentioning

confidence: 99%

Evaluation of spermatogenesis in prepubertal albino rats with date palm pollen supplement

Salman¹,

Ahmad²,

Hina³

et al. 2014

Afr. J. Pharm. Pharmacol.

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“…In vivo treatment with mAChR antagonists has been reported to induce impairment of fertility in male rats, possibly due to inhibition of sperm and semen transport from the vas deferens and seminal vesicle to the urethra during emission (Ban et al 2002;Sato et al 2005). Also, the existence of binding sites for mAChR-specific agonists in mouse and rabbit sperm (Florman and Storey 1982;Young and Laing 1991) raises the hypothesis that, besides its effects on smooth muscle contraction, ACh may interfere with sperm function.…”

Section: Machr In the Male Reproductive Tractmentioning

confidence: 99%

Muscarinic Acetylcholine Receptor Subtypes in the Male Reproductive Tract

et al. 2009

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In mammals, at least five different muscarinic acetylcholine receptor subtypes (mAChRs; M(1)-M(5)) are known to be widely expressed and distributed in different tissues from different species. They mediate distinct physiological functions according to their location and receptor subtype. Multiple events are associated with the regulation of intracellular signaling by mAChRs, and a coordinated balance of the molecular mechanisms governing receptor signaling, desensitization, resensitization, and mitogenic signaling is known to occur in various cell types. Most of the actions of acetylcholine (ACh) in the male reproductive tract are induced by its effects on mAChRs, but the role of specific mAChR subtypes on male reproductive function and fertility are still not well understood. The rat efferent ductules and epididymis are androgen-dependent tissues of the male reproductive tract, with important roles in the process to form a viable and fertile sperm. In the present study, aspects of the expression, localization, and potential function of mAChR subtypes in rat efferent ductules and epididymis are reviewed. Furthermore, evidences for the implication of mAChRs in the regulation of protein synthesis and secretion in these tissues are presented. Taken together, the studies contribute to our understanding about physiological aspects of mAChR and mechanisms by which the cholinergic system affects male reproduction.

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“…The latter signaling pathway seems to play a major role in the autocrine functions of mAChRs in terms of the control of cell growth or proliferation, secretion by epithelial cells, and apoptosis (for review see Eglen 2006). The importance of mAChRs for fertility has been revealed by the demonstration that mAChR antagonists impair fertility in male rats (Ban et al 2002, Sato et al 2005, but the underlying mechanisms that cause this infertility remain unclear. The study of the expression, localization and function of mAChR subtypes in the male reproductive tract is essential to understand the role of the cholinergic nervous system in male fertility.…”

Section: Introductionmentioning

confidence: 99%

Expression and function of G-protein-coupled receptorsin the male reproductive tract

Avellar

Lazari

Porto

2009

An. Acad. Bras. Ciênc.

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This review focuses on the expression and function of muscarinic acetylcholine receptors (mAChRs), α 1 -adrenoceptors and relaxin receptors in the male reproductive tract. The localization and differential expression of mAChR and α 1 -adrenoceptor subtypes in specific compartments of the efferent ductules, epididymis, vas deferens, seminal vesicle and prostate of various species indicate a role for these receptors in the modulation of luminal fluid composition and smooth muscle contraction, including effects on male fertility. Furthermore, the activation of mAChRs induces transactivation of the epidermal growth factor receptor (EGFR) and the Sertoli cell proliferation. The relaxin receptors are present in the testis, RXFP1 in elongated spermatids and Sertoli cells from rat, and RXFP2 in Leydig and germ cells from rat and human, suggesting a role for these receptors in the spermatogenic process. The localization of both receptors in the apical portion of epithelial cells and smooth muscle layers of the vas deferens suggests an involvement of these receptors in the contraction and regulation of secretion.

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Atropine-Induced Inhibition of Sperm and Semen Transport Impairs Fertility in Male Rats

Cited by 13 publications

References 13 publications

Evaluation of spermatogenesis in prepubertal albino rats with date palm pollen supplement

Evaluation of spermatogenesis in prepubertal albino rats with date palm pollen supplement

Muscarinic Acetylcholine Receptor Subtypes in the Male Reproductive Tract

Expression and function of G-protein-coupled receptorsin the male reproductive tract

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