2000
DOI: 10.1016/s0022-2275(20)32053-8
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Mechanism of the stimulatory action of okadaic acid on lipolysis in rat fat cells

Abstract: Okadaic acid was found to induce concentrationand time-dependent lipolysis in rat fat cells in the absence of lipolytic hormones, but it did not significantly increase the total hormone-sensitive lipase (HSL) activity in these fat cells, the activity of HSL extracted from fat layer and that of HSL in the supernatant of homogenized fat cells. Western blotting of fat cell homogenate fractions with an antiserum raised against synthetic peptide derived from rat HSL showed that HSL protein shifted from the supernat… Show more

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Cited by 29 publications
(3 citation statements)
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“…It is key enzyme of thiol (SH) group and is highly susceptible to the free radicals (Murugesan et al, 2013). Decreased activities of these enzymes alter mitochondrial substrate oxidation, resulting in decreased substratum oxidation that effect the transfering of reducing-equivalents, as a result it depletes cellular energy (Morimoto et al, 2000). However, in the present study, co-treatment of GM with CAS reversed the enzyme's activity.…”
Section: Discussioncontrasting
confidence: 53%
“…It is key enzyme of thiol (SH) group and is highly susceptible to the free radicals (Murugesan et al, 2013). Decreased activities of these enzymes alter mitochondrial substrate oxidation, resulting in decreased substratum oxidation that effect the transfering of reducing-equivalents, as a result it depletes cellular energy (Morimoto et al, 2000). However, in the present study, co-treatment of GM with CAS reversed the enzyme's activity.…”
Section: Discussioncontrasting
confidence: 53%
“…Decreased activities of these enzymes affect the mitochondrial substrate oxidation, resulting in decreased oxidation of substrates and decreased rate of transfer of reducing equivalents to molecular oxygen, thereby depleting cellular energy. 56 ARB pretreatment (50 mg/kg bw) restored the activities of these enzymes to near normal in the cardiac mitochondria.…”
Section: Discussionmentioning
confidence: 92%
“…Also, if this model is correct, it is difficult to see how the cytosolic lipase acquires access to the core lipids. (d) Finally, McGookey and Anderson (1983) have reported whorls of bilayered membranes at specialized sites on the droplet surface, and provided evidence that these were the sites of core lipid hydrolysis by hormone-sensitive lipase (Brasaemle et al, 2000;Morimoto et al, 2000). This hydrolysis results in a surplus of envelope, which may then fold back upon itself.…”
Section: Lipid Dropletsmentioning
confidence: 98%