Abstract:Bavachalcone is an important active component of the traditional Chinese medicine Fructus Psoraleae. The inhibitory effect of bavachalcone on α-glucosidase activity is reported for the first time and the mechanism elucidated by molecular docking. The inhibition of α-glucosidase by bavachalcone (IC50 15.35 ± 0.57 μg/mL) was significantly superior to acarbose (IC50 2.77 ± 0.09 mg/mL). Inhibition type was mixed competitive and non-competitive. Molecular docking suggested this inhibition stems from hydrogen bonds … Show more
“…Aloe vera (L.) extracts showed potential AG and AA inhibitory activities, possibly through a competitive or non-competitive manner as reported by multiple studies in the same domain. [21,22] Hydro-ethanol (40:60) extract of Aloe vera (L.) possesses more efficacy in inhibiting the activities of said enzymes than other extracts considering acarbose as standard.…”
The present experiment was conducted to get the most effective extract of Aloe vera (L.) leaf gel for the correction of testicular hypo-function in diabetic rat. A single-time injection (intramuscular) of Streptozotocin at 4 mg/100 g body weight was given for diabetes induction. Treatment with aqueous or ethanol or methanol or hydro-ethanol (40:60) or hydro-methanol (40:60) extract of Aloe vera (L.) was continued for 28 days. Rats were euthanized and sacrificed on 29th day. Fasting blood glucose level, hexokinase activity, androgenic key enzyme activities, and markers of oxidative stress were assessed. Concentration of the sperm per milliliter of epididymal washed fluid, sperm motility, serum testosterone, plasma insulin levels, lipid, and metabolic toxicity sensors were also measured. Significant amelioration (p˂0.05) of the negatively deviated above-mentioned parameters and the disrupted histomorphology of testicles towards vehicle-treated control were noted after uninterrupted 28 days of treatment to diabetic rat with the mentioned extracts of Aloe vera (L.). Highest percentage of recovery in the adopted sensors was noted in the hydro-ethanol extract-treated diabetes group than others. Hydro-ethanol extract of the said plant part is potent among all other extracts for correcting such hypo-function of testicles in diabetes.
“…Aloe vera (L.) extracts showed potential AG and AA inhibitory activities, possibly through a competitive or non-competitive manner as reported by multiple studies in the same domain. [21,22] Hydro-ethanol (40:60) extract of Aloe vera (L.) possesses more efficacy in inhibiting the activities of said enzymes than other extracts considering acarbose as standard.…”
The present experiment was conducted to get the most effective extract of Aloe vera (L.) leaf gel for the correction of testicular hypo-function in diabetic rat. A single-time injection (intramuscular) of Streptozotocin at 4 mg/100 g body weight was given for diabetes induction. Treatment with aqueous or ethanol or methanol or hydro-ethanol (40:60) or hydro-methanol (40:60) extract of Aloe vera (L.) was continued for 28 days. Rats were euthanized and sacrificed on 29th day. Fasting blood glucose level, hexokinase activity, androgenic key enzyme activities, and markers of oxidative stress were assessed. Concentration of the sperm per milliliter of epididymal washed fluid, sperm motility, serum testosterone, plasma insulin levels, lipid, and metabolic toxicity sensors were also measured. Significant amelioration (p˂0.05) of the negatively deviated above-mentioned parameters and the disrupted histomorphology of testicles towards vehicle-treated control were noted after uninterrupted 28 days of treatment to diabetic rat with the mentioned extracts of Aloe vera (L.). Highest percentage of recovery in the adopted sensors was noted in the hydro-ethanol extract-treated diabetes group than others. Hydro-ethanol extract of the said plant part is potent among all other extracts for correcting such hypo-function of testicles in diabetes.
“…Trilobatin and GA can interact with the hydrophobic amino acids tryptophan and phenylalanine, which confirms that trilobatin and GA can bind to the active site of α-glucosidase hydrophobic, resulting in reduced fluorescence quenching. 62 In addition, the formation of hydrogen bonds may reduce hydrophilicity while increasing the hydrophobicity of α-glucosidase, which helps increase the stability of the complex. Compared to GA, trilobatin forms more hydrogen bonds with α-glucosidase by the individual glycosidic groups on the A ring, resulting in enhanced inhibition of α-glucosidase by CT. Hua et al showed that an increase in molecular size, polarity, and nonplanar structure may reduce the inhibition of α-glucosidase, 63 so the two glucuronic acids linked by glycyrrhizic acid may lead to steric obstruction and reduce the affinity for α-glucosidase.…”
Section: Molecular Docking Of Sweetener With α-Glucosidase and α-Amylasementioning
“…With increasing attention paid to the benefits for Qingzhuan Dark Tea's human bodies, such as reducing weight and fat (Monobe et al, 2008), antioxidant ability (Xia, 2019), improvement of intestinal function (Li, 2018), and bacteriostatic effect (Mao, 2019). One of the main components of Qingzhuan Dark Tea is Polysaccharides, which is also one of the main components that play its physiological role (Ting et al, 2019). At present-day, lots of studies have verified that polysaccharides from Qingzhuan Dark Tea have the antioxidant ability and competitive inhibition of α-glycosidase activity (Ting et al, 2019).…”
The effective selenylated polysaccharides (refer as TPS-Se) with an average molecular weight (M W ) of 3.109 × 104 Da was synthesized by the reduction of sodium selenite with ascorbic acid in the presence of Qingzhuan Dark Tea polysaccharides (TPS). The selenium (Se) content of TPS-Se was up to 37.05%. The differences between TPS and TPS-Se were compared in morphology, structure, antioxidant activity, and molecular weight using FT-IR, Raman spectroscopy, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS), SEM, AFM, and Integrated thermal gravimetric analysis. The results indicated that Se 4+ was reduced to Se 0 by ascorbic acid. Further, the Se 0 atoms aggregate into Se nuclei and bind to the C=O from TPS. Also, TPS-Se exhibited higher DPPH and ABTS radical-scavenging abilities than TPS. This difference may be attributed to the fact that Se has good antioxidant activity. The test results demonstrated that TPS-Se was prepared successfully by reducing sodium selenite with ascorbic acid, which exhibited a higher radical-scavenging ability. Finally, LD 50 value of 136.89 mg/kg, with a 95% confidence interval of 102.82~182.82 mg/kg, was determined by gavage administration for TPS-Se's.
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