<i>In vitro</i> inhibitory effect of five natural sweeteners on α-glucosidase and α-amylase

Jiang, Jiequn; Fan, Heliang; Zhou, Jie; Qin, Jingkai; Qin, Zhongyi; Chen, Mei; Shen, Yuanyuan; Liu, Xiaoling

doi:10.1039/d3fo05234f

Cited by 1 publication

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“…The absorbance was measured with and without at 540 nm in a UV spectrophotometer, and three parallel experiments were performed for each group using the double-inverse graphing method. Equations (2-4) were used to determine and calculate the competition mode for α-amylase (Jiang et al, 2024).…”

Section: Study On the Competition Mode Of α-Amylase By Pspmentioning

confidence: 99%

Structural characterization and hypoglycemic effect of polysaccharides of Polygonatum sibiricum

Wang,

Yang,

Shen

et al. 2024

Journal of Food Science

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Polygonatum sibiricum polysaccharide (PSP) was extracted and purified from raw material obtained from P. sibiricum. The structural features of PSP were investigated by Congo red, circular dichroism spectrum, high‐performance gel permeation chromatography, scanning electron microscope, atomic force microscope, ultraviolet spectroscopy, and Fourier transform infrared spectroscopy analysis. In vitro simulations were conducted to investigate the kinetics of PSP enzyme inhibition. Moreover, a type II diabetes mouse model (T2DM) with streptozotocin‐induced insulin resistance was established, and the indexes of lipid quadruple, insulin resistance index, oral glucose tolerance (OGTT), organ index, and pancreatic morphology of model mice were measured. The results showed that PSP mainly consists of monosaccharides, such as mannose, glucose, galactose, xylose, and arabinose. It also has a β‐glycosidic bond of a pyranose ring and an irregular reticulated aggregated structure with a triple helix. In vitro enzyme inhibition assays revealed that PSP acts as a reversible competitive inhibitor of α‐glucosidase and α‐amylase. Furthermore, PSP was found to reduce insulin resistance index, increase OGTT and serum insulin levels, decrease free fatty acid content to improve lipid metabolism, and lower glycated serum protein content to enhance glucose metabolism in T2DM mice, thereby leading to a reduction in blood glucose concentration. Additionally, PSP exhibited reparative effects on the damaged liver tissue cells and pancreatic tissue in T2DM mice. The experiment results provide a preliminary basis for the therapeutic mechanism of PSP about type II diabetes and a theoretical reference for application in food and pharmaceutical development.

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Section: Study On the Competition Mode Of α-Amylase By Pspmentioning

confidence: 99%

Structural characterization and hypoglycemic effect of polysaccharides of Polygonatum sibiricum

Wang,

Yang,

Shen

et al. 2024

Journal of Food Science

View full text Add to dashboard Cite

show abstract

In vitro inhibitory effect of five natural sweeteners on α-glucosidase and α-amylase

Abstract: Hydrogen bonding and hydrophobic interactions are the primary forces between CT, CR and GA and α-glucosidase/α-amylase.

Cited by 1 publication

References 54 publications

Structural characterization and hypoglycemic effect of polysaccharides of Polygonatum sibiricum

Structural characterization and hypoglycemic effect of polysaccharides of Polygonatum sibiricum

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