Mechanism of Formation of Hexosemonophosphate in Muscle and Isolation of a New Phosphate Ester

Cori, Carl F.; Cori, Gerty T.

doi:10.3181/00379727-34-8759p

Cited by 122 publications

(41 citation statements)

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“…The reaction mixtures also contained 10 ml. of a saturated solution of the calcium salt of fructose-6-phosphate, 2 that the differences in activity of potato phosphatase from potatoes stored at 00 and 25°were negligible. Phosphatase activity was, further, completely suppressed at 00 C.…”

Section: Activitymentioning

confidence: 95%

“…Amylose produces deep blue color with iodine and is the form of starch produced initially by the action of phosphorylase on Cori ester. The amylopectin fraction is less soluble, gives a highly viscous, opalescent solution in H20, produces a violet-red or reddish color with iodine and is formed of chains of glucose residues linked through [1][2][3][4] linkages, but with frequent branches at position 6 of the gluco-pyranose residues. In view of these properties of the starch fractions, the change in color of the starch iodine complex after attainment of phosphate equilibrium suggests and gives some support to the theory that the enzyme preparation contains the "Q" factor of HAWORTH and co-workers (11) which enables phosphorylase to form 1,6-glycosidic linkages.…”

Section: Phosphorylated Inter-mediatesmentioning

confidence: 99%

“…In view of these properties of the starch fractions, the change in color of the starch iodine complex after attainment of phosphate equilibrium suggests and gives some support to the theory that the enzyme preparation contains the "Q" factor of HAWORTH and co-workers (11) which enables phosphorylase to form 1,6-glycosidic linkages. This must involve conversion of [1][2][3][4] linkages since no net phosphate exchange is involved.…”

Section: Phosphorylated Inter-mediatesmentioning

confidence: 99%

“…In animal tissues on the other hand a great deal is known concerning these matters. CORI and CORI (2) isolated from frog muscle glucose-l-PO4 or Cori ester as the iniitial product of phosphorolysis of glycogen. Later they obtained the same ester from rabbit muscle (3) and showed that the presence of the enzyme phosphorylase, is responsible for the reaction.…”

Section: Introductionmentioning

confidence: 99%

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Experiments on Sucrose Formation by Potato Tubers as Influenced by Temperature

Arreguin-Lozano

Bonner

1949

Plant Physiol.

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Methods STORAGEPotatoes of the Russet varietv w-ere obtainied froimi the market and were theii randomlv divided into groups. Each grouip was stored in the dark at controlled temperature (± 1°C). SUTGAR ANALYSISSamples of potato tissue were rapidly dried in a foreed draft oveni at 700 C. The dry samples were ground in a Wileyimill to 20 mesh and aliquots were theni weighed out and extracted with 80 per cent. ethanol according to HASSID (10). After the extracts were cleared with lead acetate, aliquots were used for determiniations of reducinig sugars by the ceric sulphate method. Sucrose was determined as the increase in reducing power after hydrolysis with inivertase. 'on-sug-ar reducing substainces were determined as the redueing power in aliquots of extracts which had been previously fermented with veast. Fruietose gives a characteristic color reaction with alcoholic resorcinol in strong acid solution (16) and was determined colorimetrically in a Klett-Summerson photoelectric colorimeter. Starch was analyzed in the residue of the alcohol extraction by hvdrolysis either with amylase or with acid and titrated as glucose. In reaction mixtures, starch synthesis by phosphorvlase was followed colorimiietrieally as follows: 1 ml. aliquots were removed from the digest to 1 ml. of 16 per cent. trichloroacetic acid in order to arrest the enzymatic activity. The samples were filtered and the precipitate washed several times. Onehalf ml. of iodinie-potassiumii iodide solution was added aind the sample wvas maIde up to 25 ml. final volume. The color developed was then measured in a Klett-SuLmnierson colorimeter and compared with a calibration cuirve made for potato stareh. This calibrationi is Inot an absolute one, anid can be ulsed only for the piurpose of comparisonis withini anl experiment. PHOSPHORYLATED INTER-MEDIATESA portion of dried tuber samiiple was extracted with successive portionis of 5 per cent. trichloroacetic acid in the cold by a slight modification of the method described by UMBREIT, BURRIs and STAUFFER (18)

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Section: Activitymentioning

confidence: 95%

Section: Phosphorylated Inter-mediatesmentioning

confidence: 99%

Section: Phosphorylated Inter-mediatesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Experiments on Sucrose Formation by Potato Tubers as Influenced by Temperature

Arreguin-Lozano

Bonner

1949

Plant Physiol.

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“…A total of 5 human serum samples were stored at 4°C, -20°C, and -70°C. We measured PGM activity after varying periods of storage (1,2,3,5,14, and 30 days) following serum sampling. The PGM activity at -20°C and -70°C was stable for up to 30 days but declined between days 5 and 14 at 4°C.…”

Section: Determination Of a Normal Level Of Pgm Activitymentioning

confidence: 99%

Phosphoglucomutase Activity as a Novel Biomarker in Patients With Acute Myocardial Infarction

Nishinari

Aoyama

Ogawa

et al. 2012

Circ J

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Background: Phosphoglucomutase (PGM), a key enzyme in cellular glucose utilization and energy homeostasis, has been reported to show a relationship with oxidative stress. However, the clinical importance of PGM activity has not been investigated in patients with ischemic heart disease (IHD). The aim of the present pilot study was to clarify whether PGM activity has potential as a cardiovascular risk predictor in patients with IHD. Methods and Results:The levels of serum PGM activity in 237 patients with IHD (63 patients with acute myocardial infarction (AMI) and 174 patients with stable effort angina pectoris (EAP)) were evaluated. PGM activity was compared with levels of various myocardial, thrombosis, and inflammatory biomarkers on admission. PGM activity in the AMI group was significantly increased relative to that in the EAP group on admission (AMI, 55.5 μmol · min -1 · L -1 (U/L); EAP, 14.4 U/L (P<0.001)), and was observed to increase in parallel with well-established myocardial markers (P<0.001). Moreover, PGM activity and the lipid, thrombosis, and inflammatory biomarkers in the AMI group were higher than those in the EAP group.Conclusions: PGM activity increased with levels of myocardial, thrombosis, and inflammatory biomarkers in patients with AMI, and might be useful in diagnostic applications during the acute phase in patients with AMI. (Circ J 2012; 76: 2197 - 2203)

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The Chemistry of Glycogen

Meyer¹

1943

Advances in Enzymology - And Related Areas of Molecular Biology

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Mechanism of Formation of Hexosemonophosphate in Muscle and Isolation of a New Phosphate Ester

Cited by 122 publications

References 0 publications

Experiments on Sucrose Formation by Potato Tubers as Influenced by Temperature

Experiments on Sucrose Formation by Potato Tubers as Influenced by Temperature

Phosphoglucomutase Activity as a Novel Biomarker in Patients With Acute Myocardial Infarction

The Chemistry of Glycogen

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